Construct: sgRNA BRDN0001146882

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCAGACACAGAACGATGCAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPHK2 (56848)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77096

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000019.10 19 48629057 - SPHK2 NNNGACACAGAACGATGCAG NGG 0 1.0 Tier I
2 NC_000012.12 12 92749564 + PLEKHG7 NNNGAAACAAAACGATGCAG NGG 2 0.8667 Tier II
3 NC_000002.12 2 148718069 - EPC2 NNNGAAACAGAACGAAGCAG NGG 2 0.8442 Tier II
4 NC_000001.11 1 180732999 - XPR1 NNNGAAGCAGAACGATGCAG NGG 2 0.6555 Tier II
5 NC_000009.12 9 137428651 + NOXA1 NNNGACACAGAACGGTGCAG NGG 1 0.65 Tier II
6 NC_000002.12 2 167603278 + B3GALT1 NNNGCCACAGAATGATGCAG NGG 2 0.35 Tier II
7 NC_000020.11 20 63777898 + ZBTB46 NNNGACACAGAACCACGCAG NGG 2 0.2857 Tier II
8 NC_000001.11 1 226592790 - STUM NNNGACACAGGAAGATGCAG NGG 2 0.25 Tier II
9 NC_000003.12 3 190514350 + IL1RAP NNNGGCACAGAACGAGGCAG NGG 2 0.2492 Tier II
10 NC_000019.10 19 58209862 + ZNF274 NNNGCCACAGTACGATGCAG NGG 2 0.1538 Tier II
11 NC_000007.14 7 49779872 - VWC2 NNNGACACAGACAGATGCAG NGG 2 0.1012 Tier II
12 NC_000022.11 22 19469151 + UFD1 NNNGGCACAGAAGGATGCAG NGG 2 0.0982 Tier II
13 NC_000011.10 11 79158113 - TENM4 NNNGACACACAAGGATGCAG NGG 2 0.0545 Tier II
14 NC_000001.11 1 12112746 + TNFRSF8 NNNGACACAGCACGATGCAG NTG 2 0.0156 Tier II
15 NC_000018.10 18 46559622 + LOXHD1 NNNGACACAGAAAGATGCAG NGT 2 0.0062 Tier II
16 NC_000017.11 17 30649812 + LOC105371723 NNNGACACAGAACAATGCAG NGG 1 0.75 Tier III
17 NC_000006.12 6 85392249 + LINC02535 NNNGACACAGAGCGATACAG NGG 2 0.6741 Tier III
18 NC_000002.12 2 86863846 - ANAPC1P1 NNNGTCACAGAACAATGCAG NGG 2 0.2727 Tier III
19 NC_000002.12 2 106485124 + LOC107985931 NNNGTCACAGAACAATGCAG NGG 2 0.2727 Tier III
20 NC_000008.11 8 2561064 - LINC03021 NNNGACTCAGAAGGATGCAG NGG 2 0.0597 Tier III
21 NC_000008.11 8 76154118 + LOC102724858 NNNGACACAGCACGATGCAG NGA 2 0.0278 Tier III
22 NC_000003.12 3 113127036 + NEPRO-AS1 NNNGACACAGAAGGATGCAG NGA 2 0.0095 Tier III
23 NC_000003.12 3 113127036 + LOC124906268 NNNGACACAGAAGGATGCAG NGA 2 0.0095 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 45711436 + Sphk2 NNNGACACAGATCGATGTAG NGG 2 0.2143 Tier I
2 NC_000067.6 1 39257868 + Npas2 NNNGACACAGAGCGATGCAC NGG 2 0.3095 Tier II
3 NC_000068.7 2 75917719 - Agps NNNGACAGAGAACCATGCAG NGG 2 0.2755 Tier II
4 NC_000080.6 14 47276709 + Wdhd1 NNNGCCACAGCACGATGCAG NGG 2 0.2 Tier II
5 NC_000074.6 8 61987813 - Ddx60 NNNGACACAGAACAATGGAG NGG 2 0.1 Tier II
6 NC_000067.6 1 181794414 - Dnah14 NNNGGCACAGAAGGATGCAG NGG 2 0.0982 Tier II
7 NC_000085.6 19 48023263 - Cfap58 NNNGACACAGACGGATGCAG NGG 2 0.0359 Tier II
8 NC_000068.7 2 135739870 + Plcb4 NNNGACACAGAAAGATGCAG NGA 2 0.0267 Tier II
9 NC_000072.6 6 91857047 - Ccdc174 NNNGACACAGAACGAGGCAG NGA 2 0.024 Tier II
10 NC_000075.6 9 42552593 + Grik4 NNNGACACAGAAAGATGCAG NGT 2 0.0062 Tier II
11 NC_000078.6 12 70976227 - Psma3 NNNGACACAGAAAGATGCAG NGT 2 0.0062 Tier II
12 NC_000081.6 15 4490588 + Plcxd3 NNNGACACAGAAAGATGCAG NGT 2 0.0062 Tier II
13 NC_000084.6 18 5583076 + Gm10125 NNNGGCACAGAAAGATGCAG NGG 2 0.2769 Tier III
14 NC_000075.6 9 83023030 + Gm39383 NNNGACAGAGCACGATGCAG NGG 2 0.2571 Tier III
15 NC_000068.7 2 161411960 + Gm35374 NNNGACAGAGAACGTTGCAG NGG 2 0.1286 Tier III
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Other clones with same target sequence:

(none)