Construct: sgRNA BRDN0001146882
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCAGACACAGAACGATGCAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SPHK2 (56848)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77096
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000019.10 |
19 |
48629057 |
- |
SPHK2 |
NNNGACACAGAACGATGCAG |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000012.12 |
12 |
92749564 |
+ |
PLEKHG7 |
NNNGAAACAAAACGATGCAG |
NGG |
2 |
0.8667 |
Tier II |
3 |
NC_000002.12 |
2 |
148718069 |
- |
EPC2 |
NNNGAAACAGAACGAAGCAG |
NGG |
2 |
0.8442 |
Tier II |
4 |
NC_000001.11 |
1 |
180732999 |
- |
XPR1 |
NNNGAAGCAGAACGATGCAG |
NGG |
2 |
0.6555 |
Tier II |
5 |
NC_000009.12 |
9 |
137428651 |
+ |
NOXA1 |
NNNGACACAGAACGGTGCAG |
NGG |
1 |
0.65 |
Tier II |
6 |
NC_000002.12 |
2 |
167603278 |
+ |
B3GALT1 |
NNNGCCACAGAATGATGCAG |
NGG |
2 |
0.35 |
Tier II |
7 |
NC_000020.11 |
20 |
63777898 |
+ |
ZBTB46 |
NNNGACACAGAACCACGCAG |
NGG |
2 |
0.2857 |
Tier II |
8 |
NC_000001.11 |
1 |
226592790 |
- |
STUM |
NNNGACACAGGAAGATGCAG |
NGG |
2 |
0.25 |
Tier II |
9 |
NC_000003.12 |
3 |
190514350 |
+ |
IL1RAP |
NNNGGCACAGAACGAGGCAG |
NGG |
2 |
0.2492 |
Tier II |
10 |
NC_000019.10 |
19 |
58209862 |
+ |
ZNF274 |
NNNGCCACAGTACGATGCAG |
NGG |
2 |
0.1538 |
Tier II |
11 |
NC_000007.14 |
7 |
49779872 |
- |
VWC2 |
NNNGACACAGACAGATGCAG |
NGG |
2 |
0.1012 |
Tier II |
12 |
NC_000022.11 |
22 |
19469151 |
+ |
UFD1 |
NNNGGCACAGAAGGATGCAG |
NGG |
2 |
0.0982 |
Tier II |
13 |
NC_000011.10 |
11 |
79158113 |
- |
TENM4 |
NNNGACACACAAGGATGCAG |
NGG |
2 |
0.0545 |
Tier II |
14 |
NC_000001.11 |
1 |
12112746 |
+ |
TNFRSF8 |
NNNGACACAGCACGATGCAG |
NTG |
2 |
0.0156 |
Tier II |
15 |
NC_000018.10 |
18 |
46559622 |
+ |
LOXHD1 |
NNNGACACAGAAAGATGCAG |
NGT |
2 |
0.0062 |
Tier II |
16 |
NC_000017.11 |
17 |
30649812 |
+ |
LOC105371723 |
NNNGACACAGAACAATGCAG |
NGG |
1 |
0.75 |
Tier III |
17 |
NC_000006.12 |
6 |
85392249 |
+ |
LINC02535 |
NNNGACACAGAGCGATACAG |
NGG |
2 |
0.6741 |
Tier III |
18 |
NC_000002.12 |
2 |
86863846 |
- |
ANAPC1P1 |
NNNGTCACAGAACAATGCAG |
NGG |
2 |
0.2727 |
Tier III |
19 |
NC_000002.12 |
2 |
106485124 |
+ |
LOC107985931 |
NNNGTCACAGAACAATGCAG |
NGG |
2 |
0.2727 |
Tier III |
20 |
NC_000008.11 |
8 |
2561064 |
- |
LINC03021 |
NNNGACTCAGAAGGATGCAG |
NGG |
2 |
0.0597 |
Tier III |
21 |
NC_000008.11 |
8 |
76154118 |
+ |
LOC102724858 |
NNNGACACAGCACGATGCAG |
NGA |
2 |
0.0278 |
Tier III |
22 |
NC_000003.12 |
3 |
113127036 |
+ |
NEPRO-AS1 |
NNNGACACAGAAGGATGCAG |
NGA |
2 |
0.0095 |
Tier III |
23 |
NC_000003.12 |
3 |
113127036 |
+ |
LOC124906268 |
NNNGACACAGAAGGATGCAG |
NGA |
2 |
0.0095 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000073.6 |
7 |
45711436 |
+ |
Sphk2 |
NNNGACACAGATCGATGTAG |
NGG |
2 |
0.2143 |
Tier I |
2 |
NC_000067.6 |
1 |
39257868 |
+ |
Npas2 |
NNNGACACAGAGCGATGCAC |
NGG |
2 |
0.3095 |
Tier II |
3 |
NC_000068.7 |
2 |
75917719 |
- |
Agps |
NNNGACAGAGAACCATGCAG |
NGG |
2 |
0.2755 |
Tier II |
4 |
NC_000080.6 |
14 |
47276709 |
+ |
Wdhd1 |
NNNGCCACAGCACGATGCAG |
NGG |
2 |
0.2 |
Tier II |
5 |
NC_000074.6 |
8 |
61987813 |
- |
Ddx60 |
NNNGACACAGAACAATGGAG |
NGG |
2 |
0.1 |
Tier II |
6 |
NC_000067.6 |
1 |
181794414 |
- |
Dnah14 |
NNNGGCACAGAAGGATGCAG |
NGG |
2 |
0.0982 |
Tier II |
7 |
NC_000085.6 |
19 |
48023263 |
- |
Cfap58 |
NNNGACACAGACGGATGCAG |
NGG |
2 |
0.0359 |
Tier II |
8 |
NC_000068.7 |
2 |
135739870 |
+ |
Plcb4 |
NNNGACACAGAAAGATGCAG |
NGA |
2 |
0.0267 |
Tier II |
9 |
NC_000072.6 |
6 |
91857047 |
- |
Ccdc174 |
NNNGACACAGAACGAGGCAG |
NGA |
2 |
0.024 |
Tier II |
10 |
NC_000075.6 |
9 |
42552593 |
+ |
Grik4 |
NNNGACACAGAAAGATGCAG |
NGT |
2 |
0.0062 |
Tier II |
11 |
NC_000078.6 |
12 |
70976227 |
- |
Psma3 |
NNNGACACAGAAAGATGCAG |
NGT |
2 |
0.0062 |
Tier II |
12 |
NC_000081.6 |
15 |
4490588 |
+ |
Plcxd3 |
NNNGACACAGAAAGATGCAG |
NGT |
2 |
0.0062 |
Tier II |
13 |
NC_000084.6 |
18 |
5583076 |
+ |
Gm10125 |
NNNGGCACAGAAAGATGCAG |
NGG |
2 |
0.2769 |
Tier III |
14 |
NC_000075.6 |
9 |
83023030 |
+ |
Gm39383 |
NNNGACAGAGCACGATGCAG |
NGG |
2 |
0.2571 |
Tier III |
15 |
NC_000068.7 |
2 |
161411960 |
+ |
Gm35374 |
NNNGACAGAGAACGTTGCAG |
NGG |
2 |
0.1286 |
Tier III |
Other clones with same target sequence:
(none)