Construct: sgRNA BRDN0001146892
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCTGCCTTACAACGAGAAGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CSF1R (1436)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77039
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
150061756 |
- |
CSF1R |
NNNGCCTTACAACGAGAAGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
229482677 |
+ |
DNER |
NNNGCCCTACAAAGAGAAGT |
NGG |
2 |
0.2644 |
Tier II |
| 3 |
NC_000003.12 |
3 |
143814877 |
+ |
SLC9A9 |
NNNGCCTTACACCAAGAAGT |
NGG |
2 |
0.1974 |
Tier II |
| 4 |
NC_000002.12 |
2 |
102010606 |
- |
IL1R2 |
NNNTCCTTACAACGAGCAGT |
NGG |
2 |
0.0642 |
Tier II |
| 5 |
NC_000004.12 |
4 |
7430058 |
- |
SORCS2 |
NNNTCCTTACAACGAGAAGC |
NGG |
2 |
0.0331 |
Tier II |
| 6 |
NC_000003.12 |
3 |
64925677 |
- |
ADAMTS9-AS2 |
NNNTCCTTCCAACGAGAAGT |
NGG |
2 |
0.2078 |
Tier III |
| 7 |
NC_000003.12 |
3 |
64925677 |
- |
LOC105377124 |
NNNTCCTTCCAACGAGAAGT |
NGG |
2 |
0.2078 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000084.6 |
18 |
61124293 |
+ |
Csf1r |
NNNGCCCTACAATGAGAAGT |
NGG |
2 |
0.4812 |
Tier I |
| 2 |
NC_000085.6 |
19 |
6168983 |
+ |
Batf2 |
NNNGCCTTACAACGAGAGGT |
NGG |
1 |
0.4 |
Tier II |
| 3 |
NC_000085.6 |
19 |
55449580 |
+ |
Vti1a |
NNNGCTTTACAAAGAGAAGT |
NGG |
2 |
0.3571 |
Tier II |
| 4 |
NC_000075.6 |
9 |
13524212 |
- |
Maml2 |
NNNTCCTTACAATGAGAAGT |
NGG |
2 |
0.2545 |
Tier II |
| 5 |
NC_000070.6 |
4 |
144889560 |
- |
Vps13d |
NNNGCCTTACAATTAGAAGT |
NGG |
2 |
0.1867 |
Tier II |
| 6 |
NC_000084.6 |
18 |
61124293 |
+ |
Gm52352 |
NNNGCCCTACAATGAGAAGT |
NGG |
2 |
0.4812 |
Tier III |
Other clones with same target sequence:
(none)
