Construct: sgRNA BRDN0001146894
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAGTGTCCCTTATGGCTCAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ADCK1 (57143)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76086
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000014.9 |
14 |
77822490 |
+ |
ADCK1 |
NNNTGTCCCTTATGGCTCAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
84520961 |
+ |
RALYL |
NNNTGCCTCTTATGGCTCAG |
NGG |
2 |
0.7955 |
Tier II |
| 3 |
NC_000008.11 |
8 |
144586555 |
- |
ARHGAP39 |
NNNTGTCACTAATGGCTCAG |
NGG |
2 |
0.4875 |
Tier II |
| 4 |
NC_000020.11 |
20 |
20121895 |
+ |
CFAP61 |
NNNTGTCCTTTATGGCTAAG |
NGG |
2 |
0.4712 |
Tier II |
| 5 |
NC_000013.11 |
13 |
21531923 |
+ |
MICU2 |
NNNTCTCCCTTATGGCACAG |
NGG |
2 |
0.419 |
Tier II |
| 6 |
NC_000001.11 |
1 |
41928303 |
+ |
HIVEP3 |
NNNTGTCCCTTATAGCACAG |
NGG |
2 |
0.4 |
Tier II |
| 7 |
NC_000006.12 |
6 |
39851246 |
+ |
DAAM2 |
NNNTGTGCCTTATGGCTTAG |
NGG |
2 |
0.3025 |
Tier II |
| 8 |
NC_000009.12 |
9 |
137816671 |
- |
EHMT1 |
NNNTTTCCCTTATGACTCAG |
NGG |
2 |
0.2824 |
Tier II |
| 9 |
NC_000015.10 |
15 |
39645760 |
+ |
FSIP1 |
NNNTGTCCCTGAAGGCTCAG |
NGG |
2 |
0.2769 |
Tier II |
| 10 |
NC_000007.14 |
7 |
73462466 |
- |
BAZ1B |
NNNTGTCCTTTATTGCTCAG |
NGG |
2 |
0.2333 |
Tier II |
| 11 |
NC_000001.11 |
1 |
27282022 |
+ |
WDTC1 |
NNNTGTCCTTTATGGCTCAG |
NAG |
2 |
0.2269 |
Tier II |
| 12 |
NC_000022.11 |
22 |
41396626 |
+ |
TEF |
NNNTTTCCCTTATGGCTCTG |
NGG |
2 |
0.1615 |
Tier II |
| 13 |
NC_000007.14 |
7 |
90312727 |
- |
CFAP69 |
NNNTGTCCCCTAGGGCTCAG |
NGG |
2 |
0.1391 |
Tier II |
| 14 |
NC_000001.11 |
1 |
6124447 |
- |
CHD5 |
NNNTGTCCCTTGTGGGTCAG |
NGG |
2 |
0.1111 |
Tier II |
| 15 |
NC_000012.12 |
12 |
122947624 |
+ |
ABCB9 |
NNNTGTCCCTTAGGGCTCGG |
NGG |
2 |
0.0978 |
Tier II |
| 16 |
NC_000022.11 |
22 |
39383482 |
- |
SYNGR1 |
NNNTGTCCCTTATGGCTCAG |
NGA |
1 |
0.0694 |
Tier II |
| 17 |
NC_000018.10 |
18 |
23892051 |
- |
LAMA3 |
NNNTGTCCCTTATGTCTCAG |
NGA |
2 |
0.0099 |
Tier II |
| 18 |
NC_000021.9 |
21 |
16155152 |
- |
MIR99AHG |
NNNTGCCCATTATGGCTCAG |
NGG |
2 |
0.7792 |
Tier III |
| 19 |
NC_000007.14 |
7 |
118458070 |
- |
LOC105375472 |
NNNTGTCCCTTATAGCTCAT |
NGG |
2 |
0.525 |
Tier III |
| 20 |
NC_000001.11 |
1 |
204597417 |
+ |
LOC105371692 |
NNNTGGCCCTGATGGCTCAG |
NGG |
2 |
0.2286 |
Tier III |
| 21 |
NC_000002.12 |
2 |
191202924 |
+ |
LOC105373804 |
NNNAGTCCCTTATGGCTCAG |
NTG |
2 |
0.0186 |
Tier III |
| 22 |
NC_000002.12 |
2 |
226465243 |
+ |
LOC105373915 |
NNNTGTCCCTTATGTCTCAG |
NGA |
2 |
0.0099 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000074.6 |
8 |
71389004 |
- |
Ushbp1 |
NNNTGTCTCTTATAGCTCAG |
NGG |
2 |
0.6563 |
Tier I |
| 2 |
NC_000078.6 |
12 |
88371750 |
+ |
Adck1 |
NNNTGTCCCATATGGCTCTG |
NGG |
2 |
0.4615 |
Tier I |
| 3 |
NC_000077.6 |
11 |
106798047 |
- |
Cep95 |
NNNTGCCTCTTATGGCTCAG |
NGG |
2 |
0.7955 |
Tier II |
| 4 |
NC_000067.6 |
1 |
89814837 |
+ |
Agap1 |
NNNTGTCCCTATTGGCTCAG |
NGG |
2 |
0.25 |
Tier II |
| 5 |
NC_000075.6 |
9 |
51291227 |
+ |
1810046K07Rik |
NNNTGTTCCTTAGGGCTCAG |
NGG |
2 |
0.212 |
Tier II |
| 6 |
NC_000075.6 |
9 |
48251430 |
+ |
Nxpe4 |
NNNTCTCCCTTATGGCTCAG |
NAG |
2 |
0.2037 |
Tier II |
| 7 |
NC_000067.6 |
1 |
158958631 |
- |
Pappa2 |
NNNTGTCCCTTGTGCCTCAG |
NGG |
2 |
0.197 |
Tier II |
| 8 |
NC_000068.7 |
2 |
91320060 |
+ |
1110051M20Rik |
NNNTGTGCCTGATGGCTCAG |
NGG |
2 |
0.1882 |
Tier II |
| 9 |
NC_000076.6 |
10 |
119553412 |
+ |
Grip1 |
NNNTGTCCCTTTTGGCACAG |
NGG |
2 |
0.1778 |
Tier II |
| 10 |
NC_000068.7 |
2 |
102628804 |
+ |
Pamr1 |
NNNTGTCACTTATTGCTCAG |
NGG |
2 |
0.1733 |
Tier II |
| 11 |
NC_000079.6 |
13 |
84294083 |
- |
Tmem161b |
NNNTGTCCCTTATGGTACAG |
NGG |
2 |
0.1641 |
Tier II |
| 12 |
NC_000069.6 |
3 |
32915427 |
+ |
Usp13 |
NNNTGGCCCTTATGGCTCAG |
NAG |
2 |
0.1481 |
Tier II |
| 13 |
NC_000076.6 |
10 |
121997793 |
- |
Srgap1 |
NNNTGTACCTTATGTCTCAG |
NGG |
2 |
0.1071 |
Tier II |
| 14 |
NC_000073.6 |
7 |
144424788 |
+ |
Shank2 |
NNNTGTCCCTTCTGTCTCAG |
NGG |
2 |
0.0376 |
Tier II |
| 15 |
NC_000077.6 |
11 |
101543672 |
- |
Brca1 |
NNNTGTCCCTTATGACTCAG |
NTG |
2 |
0.0367 |
Tier II |
| 16 |
NC_000067.6 |
1 |
54513043 |
- |
Pgap1 |
NNNTGTCCCTTGTGGCTCAG |
NTG |
2 |
0.0281 |
Tier II |
| 17 |
NC_000081.6 |
15 |
75473732 |
+ |
Gm34531 |
NNNTGTCCATTATGGCTCAG |
NGC |
2 |
0.019 |
Tier II |
| 18 |
NC_000077.6 |
11 |
70986467 |
- |
Dhx33 |
NNNTGACCCTTATGGCTCAG |
NGT |
2 |
0.014 |
Tier II |
| 19 |
NC_000074.6 |
8 |
122667060 |
- |
Cbfa2t3 |
NNNTGTGCCTTATGGCTCAG |
NGC |
2 |
0.0105 |
Tier II |
| 20 |
NC_000078.6 |
12 |
88371750 |
+ |
3200001D21Rik |
NNNTGTCCCATATGGCTCTG |
NGG |
2 |
0.4615 |
Tier III |
| 21 |
NC_000067.6 |
1 |
193735564 |
- |
Gm31870 |
NNNTGTCCCTGATGGCTCAA |
NGG |
2 |
0.375 |
Tier III |
| 22 |
NC_000075.6 |
9 |
51291227 |
+ |
Gm32819 |
NNNTGTTCCTTAGGGCTCAG |
NGG |
2 |
0.212 |
Tier III |
| 23 |
NC_000071.6 |
5 |
44127861 |
+ |
Gm35900 |
NNNTGTCCCTTATGCCTCAG |
NAG |
2 |
0.0707 |
Tier III |
Other clones with same target sequence:
(none)
