Construct: sgRNA BRDN0001146897
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTAACACTCCTTACTTGAGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- LATS1 (9113)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76025
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
149684369 |
+ |
LATS1 |
NNNACACTCCTTACTTGAGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
76000732 |
+ |
SV2C |
NNNACACTCCTTGCTTGATG |
NGG |
2 |
0.4348 |
Tier II |
| 3 |
NC_000013.11 |
13 |
107694207 |
- |
NALF1 |
NNNAAACTCCTTACTTGAGT |
NGG |
2 |
0.4 |
Tier II |
| 4 |
NC_000007.14 |
7 |
149255387 |
+ |
ZNF212 |
NNNCCAATCCTTACTTGAGG |
NGG |
2 |
0.2647 |
Tier II |
| 5 |
NC_000017.11 |
17 |
56111253 |
- |
ANKFN1 |
NNNTCACTCCTTACTGGAGG |
NGG |
2 |
0.2203 |
Tier II |
| 6 |
NC_000003.12 |
3 |
192853325 |
+ |
MB21D2 |
NNNACACTCCTTTCTTTAGG |
NGG |
2 |
0.075 |
Tier II |
| 7 |
NC_000006.12 |
6 |
8016110 |
- |
BLOC1S5 |
NNNAAACTCCTTACTTGAGG |
NGA |
2 |
0.0397 |
Tier II |
| 8 |
NC_000019.10 |
19 |
39468960 |
- |
SUPT5H |
NNNACACTCCTAACTTGAGG |
NGT |
2 |
0.0129 |
Tier II |
| 9 |
NC_000005.10 |
5 |
156184589 |
- |
SGCD |
NNNACACTCCTTACTTTAGG |
NGT |
2 |
0.004 |
Tier II |
| 10 |
NC_000009.12 |
9 |
133161040 |
+ |
GBGT1 |
NNNAGACTCCTTAGTTGAGG |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000006.12 |
6 |
8016110 |
- |
BLOC1S5-TXNDC5 |
NNNAAACTCCTTACTTGAGG |
NGA |
2 |
0.0397 |
Tier III |
| 12 |
NC_000006.12 |
6 |
8016110 |
- |
EEF1E1-BLOC1S5 |
NNNAAACTCCTTACTTGAGG |
NGA |
2 |
0.0397 |
Tier III |
| 13 |
NC_000012.12 |
12 |
9341511 |
+ |
LOC105369649 |
NNNACACTCCTTAATTGAGG |
NGA |
2 |
0.0243 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
7701834 |
- |
Lats1 |
NNNACACTCCTAACTTGAGG |
NGG |
1 |
0.8 |
Tier I |
| 2 |
NC_000075.6 |
9 |
45150407 |
- |
Scn4b |
NNNACACTCCTTCCTGGAGG |
NGG |
2 |
0.0729 |
Tier I |
| 3 |
NC_000086.7 |
X |
69714081 |
+ |
Aff2 |
NNNACACTACTTACTTGAGA |
NGG |
2 |
0.8036 |
Tier II |
| 4 |
NC_000081.6 |
15 |
43479697 |
+ |
Emc2 |
NNNACACTCTTTATTTGAGG |
NGG |
2 |
0.6902 |
Tier II |
| 5 |
NC_000086.7 |
X |
69504316 |
+ |
Aff2 |
NNNACACGACTTACTTGAGG |
NGG |
2 |
0.6286 |
Tier II |
| 6 |
NC_000081.6 |
15 |
25393884 |
+ |
Basp1 |
NNNAATCTCCTTACTTGAGG |
NGG |
2 |
0.4082 |
Tier II |
| 7 |
NC_000069.6 |
3 |
95617595 |
+ |
Golph3l |
NNNAGACTCCTGACTTGAGG |
NGG |
2 |
0.3 |
Tier II |
| 8 |
NC_000074.6 |
8 |
33600843 |
+ |
Ppp2cb |
NNNGCACTCCTTACTTGGGG |
NGG |
2 |
0.25 |
Tier II |
| 9 |
NC_000075.6 |
9 |
10229744 |
+ |
Cntn5 |
NNNACACTCCTTGCTGGAGG |
NGG |
2 |
0.2258 |
Tier II |
| 10 |
NC_000068.7 |
2 |
180995982 |
- |
Col20a1 |
NNNACACTCCTTACTTGAGG |
NGC |
1 |
0.0222 |
Tier II |
| 11 |
NC_000079.6 |
13 |
42193746 |
+ |
Gm32778 |
NNNACAGTCCTTACTTGAGG |
NGG |
1 |
0.4706 |
Tier III |
| 12 |
NC_000076.6 |
10 |
31033965 |
- |
Gm30676 |
NNNATACTCCTTACTTGATG |
NGG |
2 |
0.4242 |
Tier III |
| 13 |
NC_000078.6 |
12 |
101261390 |
+ |
Gm18795 |
NNNACTGTCCTTACTTGAGG |
NGG |
2 |
0.3361 |
Tier III |
| 14 |
NC_000085.6 |
19 |
50706514 |
+ |
Gm26629 |
NNNACACACCTTACTTGAGG |
NAG |
2 |
0.2074 |
Tier III |
| 15 |
NC_000084.6 |
18 |
32590398 |
- |
Gm35480 |
NNNACGCTCCTTCCTTGAGG |
NGG |
2 |
0.1504 |
Tier III |
Other clones with same target sequence:
(none)
