Construct: sgRNA BRDN0001146906
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGATTTAGCTATTCCCACGC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIK3CA (5290)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77417
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
179210556 |
+ |
PIK3CA |
NNNTTTAGCTATTCCCACGC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000023.11 |
X |
153334610 |
- |
ZNF275 |
NNNTTTAGCGATTCCCACCC |
NGG |
2 |
0.2241 |
Tier II |
| 3 |
NC_000003.12 |
3 |
54422912 |
- |
CACNA2D3 |
NNNTTTAGCTTTTCCCACAC |
NGG |
2 |
0.2198 |
Tier II |
| 4 |
NC_000003.12 |
3 |
68502944 |
- |
TAFA1 |
NNNTTTAGCTATTCCCCCGC |
NGG |
1 |
0.1765 |
Tier II |
| 5 |
NC_000014.9 |
14 |
69974769 |
+ |
SMOC1 |
NNNTTTTGCTATTCCCAGGC |
NGG |
2 |
0.0583 |
Tier II |
| 6 |
NC_000011.10 |
11 |
28504701 |
- |
METTL15 |
NNNTTTAGCTTTTGCCACGC |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000003.12 |
3 |
68502944 |
- |
LOC105377146 |
NNNTTTAGCTATTCCCCCGC |
NGG |
1 |
0.1765 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
101811049 |
+ |
Fbln5 |
NNNTTTAGCTATTCCCACAT |
NGG |
2 |
0.2143 |
Tier II |
| 2 |
NC_000067.6 |
1 |
189798367 |
+ |
Ptpn14 |
NNNTTTAGCTTGTCCCACGC |
NGG |
2 |
0.1538 |
Tier II |
| 3 |
NC_000076.6 |
10 |
105800863 |
- |
Mettl25 |
NNNTTTACCTATTCCCAGGC |
NGG |
2 |
0.0821 |
Tier II |
Other clones with same target sequence:
(none)
