Construct: sgRNA BRDN0001146908
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AACGGGATACAGACTCGTTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- XYLB (9942)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77649
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
38376156 |
- |
XYLB |
NNNGGGATACAGACTCGTTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000020.11 |
20 |
62964810 |
+ |
SLC17A9 |
NNNGGAATACACACTCGTTG |
NGG |
2 |
0.5294 |
Tier II |
| 3 |
NC_000001.11 |
1 |
169017361 |
- |
LINC00970 |
NNNAGGATACAGACTCCTTG |
NGG |
2 |
0.2118 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
96972905 |
- |
Spsb4 |
NNNGGGATACAGGCTAGTTG |
NGG |
2 |
0.6522 |
Tier II |
| 2 |
NC_000073.6 |
7 |
64688357 |
- |
Apba2 |
NNNGGGATAAAGGCTCGTTG |
NGG |
2 |
0.5652 |
Tier II |
| 3 |
NC_000070.6 |
4 |
61449291 |
- |
Mup-ps12 |
NNNGGGACACAGACTTGTTG |
NGG |
2 |
0.3077 |
Tier II |
| 4 |
NC_000077.6 |
11 |
75535635 |
+ |
Slc43a2 |
NNNGGGCTACAGACACGTTG |
NGG |
2 |
0.2533 |
Tier II |
| 5 |
NC_000085.6 |
19 |
42321110 |
+ |
Crtac1 |
NNNGGGAAACAGACTCGTGG |
NGG |
2 |
0.2 |
Tier II |
| 6 |
NC_000067.6 |
1 |
186691872 |
+ |
Tgfb2 |
NNNGGGATACAGTCTCGTTC |
NGG |
2 |
0.1286 |
Tier II |
| 7 |
NC_000070.6 |
4 |
15002244 |
- |
Necab1 |
NNNGGGATACAGACTTGTTG |
NGA |
2 |
0.0214 |
Tier II |
| 8 |
NC_000079.6 |
13 |
48729422 |
+ |
Gm36784 |
NNNGGGATCCAGACTAGTTG |
NGG |
2 |
0.5714 |
Tier III |
| 9 |
NC_000071.6 |
5 |
138910540 |
+ |
Gm31891 |
NNNGGGATACAGACTGGTTG |
NTG |
2 |
0.006 |
Tier III |
Other clones with same target sequence:
(none)
