Construct: sgRNA BRDN0001146911

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TACTGCATGGGAGTTAACTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AK9 (221264)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77689

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000006.12 6 109563683 - AK9 NNNTGCATGGGAGTTAACTG NGG 0 1.0 Tier I
2 NC_000009.12 9 8887084 + PTPRD NNNTACATGTGAGTTAACTG NGG 2 0.7042 Tier II
3 NC_000020.11 20 48651197 + PREX1 NNNTGCACGGGAGTAAACTG NGG 2 0.5789 Tier II
4 NC_000005.10 5 741907 - ZDHHC11B NNNTGCGTGGGAGTTAAATG NGG 2 0.3801 Tier II
5 NC_000009.12 9 83391976 - FRMD3 NNNTGCATGCGAATTAACTG NGG 2 0.3692 Tier II
6 NC_000022.11 22 50032563 - TTLL8 NNNTGCAGGGGAGTTAACTC NGG 2 0.3143 Tier II
7 NC_000001.11 1 54272195 + SSBP3 NNNTGCCTGGGAGTTAACTT NGG 2 0.3062 Tier II
8 NC_000017.11 17 39828338 + IKZF3 NNNTGTATGGGAGTTGACTG NGG 2 0.1786 Tier II
9 NC_000003.12 3 1298721 + CNTN6 NNNTGCATGTGAGTTACCTG NGG 2 0.1434 Tier II
10 NC_000014.9 14 71367843 - SIPA1L1 NNNTGCAGGGGAGTTACCTG NGG 2 0.1294 Tier II
11 NC_000012.12 12 25120919 + DNAI7 NNNTGCATAGGAGTTACCTG NGG 2 0.1134 Tier II
12 NC_000008.11 8 39227537 + ADAM32 NNNTGCATGGGAGCTGACTG NGG 2 0.0549 Tier II
13 NC_000006.12 6 143176654 - AIG1 NNNTGCATGGGAGCTAAGTG NGG 2 0.0381 Tier II
14 NC_000003.12 3 120662468 + HGD NNNTGCATGGGAGTCATCTG NGG 2 0.0364 Tier II
15 NC_000005.10 5 168109700 - TENM2 NNNTGCATGGGAGTCATCTG NGG 2 0.0364 Tier II
16 NC_000008.11 8 28480899 + FBXO16 NNNTGCATGGGAGTTGACTG NGA 2 0.0134 Tier II
17 NC_000023.11 X 41657087 + CASK NNNTGCATGGGAGTGGACTG NGG 2 0.0096 Tier II
18 NC_000001.11 1 47882265 + TRABD2B NNNTGCATGGGACTTCACTG NGG 2 0.0 Tier II
19 NC_000015.10 15 77684773 - LINGO1 NNNTGCATGGGAGGTCACTG NGG 2 0.0 Tier II
20 NC_000005.10 5 89253780 + MEF2C-AS1 NNNTGCCTGGGAGATAACTG NGG 2 0.2708 Tier III
21 NC_000003.12 3 187491200 + LOC124909471 NNNTGCATGGGAGTTGAGTG NGG 2 0.0256 Tier III
22 NC_000001.11 1 24151759 + LOC124903879 NNNTGCATGGGAGTAAACTG NTG 2 0.0226 Tier III
23 NC_000023.11 X 41657087 + LOC124905180 NNNTGCATGGGAGTGGACTG NGG 2 0.0096 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000072.6 6 12555699 - Thsd7a NNNTGCATGGGAGATGACTG NGG 2 0.119 Tier I
2 NC_000075.6 9 76167080 - Gfral NNNTGCATGGCAGTTACCTG NGG 2 0.0756 Tier I
3 NC_000067.6 1 134342789 - Ppfia4 NNNTGCAGGGAAGTTAACTG NGG 2 0.7333 Tier II
4 NC_000078.6 12 55260914 + Srp54c NNNTGCATAGGAGTTAAATG NGG 2 0.3462 Tier II
5 NC_000074.6 8 102766075 - Cdh11 NNNTGCATGGAAGTTAACTG NAG 2 0.2593 Tier II
6 NC_000078.6 12 82627953 + Rgs6 NNNTGGCTGGGAGTTAACTG NGG 2 0.2188 Tier II
7 NC_000075.6 9 21813857 + Dock6 NNNTGCATGGGAGTTAACAT NGG 2 0.2 Tier II
8 NC_000076.6 10 86906640 + Stab2 NNNTGCTTGGGACTTAACTG NGG 2 0.1842 Tier II
9 NC_000076.6 10 122755823 - Ppm1h NNNTGCCTGGGTGTTAACTG NGG 2 0.1458 Tier II
10 NC_000075.6 9 101954559 + Ephb1 NNNTGCATGCGTGTTAACTG NGG 2 0.1333 Tier II
11 NC_000084.6 18 65681791 + Zfp532 NNNTGCTTGGGATTTAACTG NGG 2 0.1313 Tier II
12 NC_000079.6 13 59032649 + Ntrk2 NNNTGCATGGGAGCTATCTG NGG 2 0.0381 Tier II
13 NC_000084.6 18 42683525 + Ppp2r2b NNNTGCATGCGAGTGAACTG NGG 2 0.02 Tier II
14 NC_000075.6 9 115116693 + Osbpl10 NNNTGCATGGAAGTTTACTG NGG 2 0.0 Tier II
15 NC_000075.6 9 7082388 - Dync2h1 NNNTGCATGGCAGTTTACTG NGG 2 0.0 Tier II
16 NC_000073.6 7 100179469 + Kcne3 NNNTGCATGGGAGGAAACTG NGG 2 0.0 Tier II
17 NC_000083.6 17 84693957 - Abcg8 NNNTGTATGGGAGTTCACTG NGG 2 0.0 Tier II
18 NC_000073.6 7 83819421 + Gm39043 NNNTGCATGGGAGTTGACTG NGA 2 0.0134 Tier III
Download CSV

Other clones with same target sequence:

(none)