Construct: sgRNA BRDN0001146915
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACCTAAGGAATGCACTTCAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NME5 (8382)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76573
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
138128537 |
- |
NME5 |
NNNTAAGGAATGCACTTCAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
160058715 |
- |
TTC1 |
NNNTAAGAAATACACTTCAT |
NGG |
2 |
0.9333 |
Tier II |
| 3 |
NC_000005.10 |
5 |
160058715 |
- |
PWWP2A |
NNNTAAGAAATACACTTCAT |
NGG |
2 |
0.9333 |
Tier II |
| 4 |
NC_000002.12 |
2 |
17595845 |
- |
VSNL1 |
NNNTAAAGAATGCACTTTAT |
NGG |
2 |
0.6429 |
Tier II |
| 5 |
NC_000006.12 |
6 |
162033404 |
+ |
PRKN |
NNNTAAGGAATGCACACCAT |
NGG |
2 |
0.6417 |
Tier II |
| 6 |
NC_000007.14 |
7 |
117522794 |
+ |
CFTR |
NNNGAAGGATTGCACTTCAT |
NGG |
2 |
0.5515 |
Tier II |
| 7 |
NC_000001.11 |
1 |
163216192 |
- |
RGS5 |
NNNTAAGGAGTGCTCTTCAT |
NGG |
2 |
0.2963 |
Tier II |
| 8 |
NC_000004.12 |
4 |
183506697 |
+ |
ING2 |
NNNTAAGGAATCCACTTCTT |
NGG |
2 |
0.2851 |
Tier II |
| 9 |
NC_000006.12 |
6 |
150624789 |
+ |
PLEKHG1 |
NNNTAAGGAATTCACTTCAA |
NGG |
2 |
0.2163 |
Tier II |
| 10 |
NC_000023.11 |
X |
151745201 |
- |
CNGA2 |
NNNTTAGGAATGCTCTTCAT |
NGG |
2 |
0.1939 |
Tier II |
| 11 |
NC_000014.9 |
14 |
62014000 |
- |
SYT16 |
NNNTAAGGAATGCATTTCAT |
NGG |
1 |
0.0667 |
Tier II |
| 12 |
NC_000011.10 |
11 |
110692946 |
- |
ARHGAP20 |
NNNTAAGGAATCCATTTCAT |
NGG |
2 |
0.0353 |
Tier II |
| 13 |
NC_000005.10 |
5 |
16839138 |
+ |
MYO10 |
NNNTAAGGAATGCATTTCAT |
NAG |
2 |
0.0173 |
Tier II |
| 14 |
NC_000006.12 |
6 |
65524681 |
- |
EYS |
NNNTAAGGAATTCACTTCAT |
NGC |
2 |
0.0085 |
Tier II |
| 15 |
NC_000013.11 |
13 |
95704534 |
- |
DNAJC3 |
NNNTAAGGAATTCACTTCAT |
NGC |
2 |
0.0085 |
Tier II |
| 16 |
NC_000001.11 |
1 |
172555692 |
- |
SUCO |
NNNTAAGGAATGCATTTCAT |
NTG |
2 |
0.0026 |
Tier II |
| 17 |
NC_000014.9 |
14 |
19127591 |
- |
LNCRNA-ATB |
NNNTAAGGACTGCACCTCAT |
NGG |
2 |
0.2222 |
Tier III |
| 18 |
NC_000014.9 |
14 |
19272404 |
+ |
LINC01297-DUXAP10-NBEAP6 |
NNNTAAGGACTGCACCTCAT |
NGG |
2 |
0.2222 |
Tier III |
| 19 |
NC_000007.14 |
7 |
10597733 |
- |
MGC4859 |
NNNTAAGGAATGCAATACAT |
NGG |
2 |
0.1185 |
Tier III |
| 20 |
NC_000005.10 |
5 |
89378570 |
+ |
MEF2C-AS1 |
NNNTAAGGAATGGACTTGAT |
NGG |
2 |
0.0182 |
Tier III |
| 21 |
NC_000012.12 |
12 |
20204708 |
- |
LOC105369686 |
NNNTAAGAAATGCACTTCAT |
NGT |
2 |
0.0161 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000084.6 |
18 |
34569856 |
- |
Nme5 |
NNNTCAGGAACGCACTTCAT |
NGG |
2 |
0.3333 |
Tier I |
| 2 |
NC_000068.7 |
2 |
49176254 |
+ |
Mbd5 |
NNNTAAGGAAAGCACTTAAT |
NGG |
2 |
0.4038 |
Tier II |
| 3 |
NC_000076.6 |
10 |
36694391 |
+ |
Hs3st5 |
NNNTAAGGAAAGCACTTCTT |
NGG |
2 |
0.4038 |
Tier II |
| 4 |
NC_000076.6 |
10 |
88412785 |
- |
Gnptab |
NNNTAACGAGTGCACTTCAT |
NGG |
2 |
0.3819 |
Tier II |
| 5 |
NC_000082.6 |
16 |
23254814 |
- |
St6gal1 |
NNNTAGGGAATGCTCTTCAT |
NGG |
2 |
0.381 |
Tier II |
| 6 |
NC_000079.6 |
13 |
75989657 |
+ |
Spata9 |
NNNAAAGGAAAGCACTTCAT |
NGG |
2 |
0.3571 |
Tier II |
| 7 |
NC_000083.6 |
17 |
90937355 |
+ |
Nrxn1 |
NNNAAAGGAAAGCACTTCAT |
NGG |
2 |
0.3571 |
Tier II |
| 8 |
NC_000072.6 |
6 |
18515176 |
- |
Cttnbp2 |
NNNTAAGGAAGGCACCTCAT |
NGG |
2 |
0.2667 |
Tier II |
| 9 |
NC_000074.6 |
8 |
47850759 |
- |
Wwc2 |
NNNCAAGGAATGCACTTCGT |
NGG |
2 |
0.2426 |
Tier II |
| 10 |
NC_000085.6 |
19 |
43163633 |
+ |
Hpse2 |
NNNTAAGGAAAGCACTTCAT |
NAG |
2 |
0.1944 |
Tier II |
| 11 |
NC_000072.6 |
6 |
137483037 |
+ |
Eps8 |
NNNTAATGACTGCACTTCAT |
NGG |
2 |
0.1905 |
Tier II |
| 12 |
NC_000072.6 |
6 |
97243334 |
+ |
Lmod3 |
NNNTAAGGAATGCACTTTCT |
NGG |
2 |
0.133 |
Tier II |
| 13 |
NC_000071.6 |
5 |
121617881 |
- |
Acad12 |
NNNTGAGGAATGCACTTCAG |
NGG |
2 |
0.1271 |
Tier II |
| 14 |
NC_000084.6 |
18 |
65152543 |
- |
Nedd4l |
NNNTCAGGAATGCACTTGAT |
NGG |
2 |
0.0667 |
Tier II |
| 15 |
NC_000075.6 |
9 |
110591557 |
+ |
Setd2 |
NNNTAAGGAATGGACTTCGT |
NGG |
2 |
0.0511 |
Tier II |
| 16 |
NC_000071.6 |
5 |
23929080 |
+ |
Fam126a |
NNNCAAGGAATGCATTTCAT |
NGG |
2 |
0.0431 |
Tier II |
| 17 |
NC_000070.6 |
4 |
45517036 |
+ |
Shb |
NNNTAATGAATGCACTTCAT |
NGC |
2 |
0.0127 |
Tier II |
| 18 |
NC_000076.6 |
10 |
118550237 |
+ |
Ifngas1 |
NNNGAAGGAAAGCACTTCAT |
NGG |
2 |
0.4688 |
Tier III |
| 19 |
NC_000069.6 |
3 |
82662258 |
- |
Gm29999 |
NNNTAAGGAAGGAACTTCAT |
NGG |
2 |
0.1538 |
Tier III |
| 20 |
NC_000075.6 |
9 |
70157002 |
+ |
Gm39476 |
NNNTAAGGAATGCACTGCAT |
NGT |
2 |
0.0019 |
Tier III |
Other clones with same target sequence:
(none)
