Construct: sgRNA BRDN0001146919
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCTAGCAGTACATACCACCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- AXL (558)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76702
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
41242921 |
- |
AXL |
NNNAGCAGTACATACCACCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
93098415 |
- |
MTF2 |
NNNTACAGTACATACCACCA |
NGG |
2 |
0.5515 |
Tier II |
| 3 |
NC_000020.11 |
20 |
49165694 |
- |
STAU1 |
NNNAGCAGTAAATACCACCT |
NGG |
2 |
0.45 |
Tier II |
| 4 |
NC_000017.11 |
17 |
43175363 |
- |
NBR1 |
NNNAGCAGGACATACCAACA |
NGG |
2 |
0.3333 |
Tier II |
| 5 |
NC_000013.11 |
13 |
93806493 |
+ |
GPC6 |
NNNAGGTGTACATACCACCA |
NGG |
2 |
0.2188 |
Tier II |
| 6 |
NC_000020.11 |
20 |
57251040 |
+ |
BMP7 |
NNNAGCAGCACATCCCACCA |
NGG |
2 |
0.1978 |
Tier II |
| 7 |
NC_000016.10 |
16 |
78407541 |
- |
WWOX |
NNNAGCAGTAGACACCACCA |
NGG |
2 |
0.1974 |
Tier II |
| 8 |
NC_000009.12 |
9 |
112131711 |
+ |
SUSD1 |
NNNAGCAGTTCATACCCCCA |
NGG |
2 |
0.1557 |
Tier II |
| 9 |
NC_000005.10 |
5 |
151138924 |
- |
ANXA6 |
NNNAACAGTACATACCTCCA |
NGG |
2 |
0.1156 |
Tier II |
| 10 |
NC_000007.14 |
7 |
95911419 |
- |
DYNC1I1 |
NNNAGAAGTACATACCACCA |
NTG |
2 |
0.0362 |
Tier II |
| 11 |
NC_000003.12 |
3 |
51139088 |
+ |
DOCK3 |
NNNATCAGTACATAGCACCA |
NGG |
2 |
0.015 |
Tier II |
| 12 |
NC_000016.10 |
16 |
265979 |
- |
FAM234A |
NNNAGCAGTACAGAGCACCA |
NGG |
2 |
0.013 |
Tier II |
| 13 |
NC_000008.11 |
8 |
24367264 |
+ |
ADAM7-AS1 |
NNNAGCTGTACATACCATCA |
NGG |
2 |
0.2812 |
Tier III |
| 14 |
NC_000015.10 |
15 |
97086451 |
+ |
LOC105371004 |
NNNAGCAGTAAATAACACCA |
NGG |
2 |
0.1667 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
25770728 |
+ |
Axl |
NNNAGCAGTACATACCACCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000068.7 |
2 |
154504793 |
- |
Cbfa2t2 |
NNNAGCTGTACTTACCACCA |
NGG |
2 |
0.1458 |
Tier I |
| 3 |
NC_000067.6 |
1 |
166276328 |
- |
Ildr2 |
NNNACCAGAACATACCACCA |
NGG |
2 |
0.7296 |
Tier II |
| 4 |
NC_000080.6 |
14 |
33365588 |
- |
Arhgap22 |
NNNAGCAGCAAATACCACCA |
NGG |
2 |
0.6923 |
Tier II |
| 5 |
NC_000070.6 |
4 |
128683516 |
- |
Phc2 |
NNNAGCAGTAAACACCACCA |
NGG |
2 |
0.5921 |
Tier II |
| 6 |
NC_000076.6 |
10 |
122703768 |
- |
Ppm1h |
NNNAGAAGTACATTCCACCA |
NGG |
2 |
0.4952 |
Tier II |
| 7 |
NC_000068.7 |
2 |
84599676 |
+ |
Ctnnd1 |
NNNAGCAGTACATACCACTA |
NGG |
1 |
0.4615 |
Tier II |
| 8 |
NC_000068.7 |
2 |
61634885 |
+ |
Tank |
NNNAGCAGTACATACCAACT |
NGG |
2 |
0.3231 |
Tier II |
| 9 |
NC_000075.6 |
9 |
75202843 |
- |
Myo5a |
NNNAGCAGTACATCCCATCA |
NGG |
2 |
0.1378 |
Tier II |
| 10 |
NC_000084.6 |
18 |
71324593 |
- |
Dcc |
NNNAGTAGTACATAGCACCA |
NGG |
2 |
0.0464 |
Tier II |
| 11 |
NC_000074.6 |
8 |
79340796 |
- |
Smad1 |
NNNAGCAGTACATGCCACCA |
NTG |
2 |
0.0182 |
Tier II |
| 12 |
NC_000069.6 |
3 |
152586694 |
+ |
Ak5 |
NNNAGCAGTACCTATCACCA |
NGG |
2 |
0.0175 |
Tier II |
| 13 |
NC_000069.6 |
3 |
147970496 |
+ |
Gm40175 |
NNNAGCAGTTCATGCCACCA |
NGG |
2 |
0.4118 |
Tier III |
| 14 |
NC_000082.6 |
16 |
86834854 |
+ |
Gm32624 |
NNNACCAGTCCATACCACCA |
NGG |
2 |
0.2619 |
Tier III |
| 15 |
NC_000068.7 |
2 |
67384176 |
+ |
Gm13599 |
NNNAGCAGAACAGACCACCA |
NGG |
2 |
0.2422 |
Tier III |
| 16 |
NC_000074.6 |
8 |
24386632 |
- |
Gm30633 |
NNNAGCAGTAGAAACCACCA |
NGG |
2 |
0.1731 |
Tier III |
| 17 |
NC_000070.6 |
4 |
48820434 |
+ |
Gm52718 |
NNNAGCAGCACATACCACCA |
NGC |
2 |
0.0205 |
Tier III |
Other clones with same target sequence:
(none)
