Construct: sgRNA BRDN0001146920

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCTTCATAAAACTTCACCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GRK3 (157)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77944

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000022.11 22 25644648 - GRK3 NNNTCATAAAACTTCACCTG NGG 0 1.0 Tier I
2 NC_000008.11 8 6467512 - MCPH1 NNNTGTTAAAACTTCACCTG NGG 2 0.4286 Tier II
3 NC_000005.10 5 150040062 - HMGXB3 NNNTCACACAACTTCACCTG NGG 2 0.3929 Tier II
4 NC_000017.11 17 64060902 - ERN1 NNNTCATCATACTTCACCTG NGG 2 0.3782 Tier II
5 NC_000005.10 5 118932927 + DTWD2 NNNTGATAAAACTACACCTG NGG 2 0.3714 Tier II
6 NC_000002.12 2 164557914 - GRB14 NNNTCATAAAACATCATCTG NGG 2 0.3231 Tier II
7 NC_000002.12 2 29428367 + ALK NNNTCATAAATCTTCACCTA NGG 2 0.2885 Tier II
8 NC_000012.12 12 98813170 + ANKS1B NNNTCATAAAATTTCATCTG NGG 2 0.2513 Tier II
9 NC_000006.12 6 31879840 + EHMT2 NNNTCATCAAACTTCAACTG NGG 2 0.2 Tier II
10 NC_000013.11 13 45526539 - COG3 NNNTCATCAAAGTTCACCTG NGG 2 0.1905 Tier II
11 NC_000002.12 2 73235903 + CCT7 NNNTGATAAAACTTCACCAG NGG 2 0.1714 Tier II
12 NC_000001.11 1 237282653 - RYR2 NNNTCATAAAACTTCACCAG NAG 2 0.0741 Tier II
13 NC_000003.12 3 58514401 - ACOX2 NNNTCATAAAACCTCAGCTG NGG 2 0.0464 Tier II
14 NC_000014.9 14 75943650 - TTLL5 NNNTCACAAAACTTTACCTG NGG 2 0.0458 Tier II
15 NC_000005.10 5 31499782 - DROSHA NNNTCATAAAGCTTCACCTG NGA 2 0.0451 Tier II
16 NC_000003.12 3 146535789 + PLSCR1 NNNTCATAAAACTTCACATG NGA 2 0.0374 Tier II
17 NC_000007.14 7 122539944 + CADPS2 NNNTCATAAAACTTCAACTG NTG 2 0.0182 Tier II
18 NC_000020.11 20 42687398 - PTPRT NNNTCATAAAACTTCATCTG NTG 2 0.0182 Tier II
19 NC_000002.12 2 201771774 + ALS2 NNNTCATAAAACTTCCTCTG NGG 2 0.0 Tier II
20 NC_000002.12 2 105362600 + FHL2 NNNTCATAAAACTTCTCCTG NGT 2 0.0 Tier II
21 NC_000002.12 2 105362600 + C2orf49 NNNTCATAAAACTTCTCCTG NGT 2 0.0 Tier II
22 NC_000005.10 5 178774299 - AACSP1 NNNTCATAAAACCTCACATG NGG 2 0.4251 Tier III
23 NC_000006.12 6 14427414 - LOC105374942 NNNCCATAAAACTTCACTTG NGG 2 0.416 Tier III
24 NC_000009.12 9 83724675 + LOC124902191 NNNTAATAAAACTTCACTTG NGG 2 0.3673 Tier III
25 NC_000013.11 13 76721771 - LOC105370265 NNNTCCTAAAGCTTCACCTG NGG 2 0.2955 Tier III
26 NC_000006.12 6 31879840 + EHMT2-AS1 NNNTCATCAAACTTCAACTG NGG 2 0.2 Tier III
27 NC_000003.12 3 193842052 - LINC02038 NNNTCATACAACTTCACCTG NGA 2 0.0397 Tier III
28 NC_000007.14 7 122539944 + LOC105375481 NNNTCATAAAACTTCAACTG NTG 2 0.0182 Tier III
29 NC_000020.11 20 42687398 - LOC101927159 NNNTCATAAAACTTCATCTG NTG 2 0.0182 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 112973525 + Grk3 NNNTCATAAAATTTCACCTG NGG 1 0.5385 Tier I
2 NC_000071.6 5 8652024 - Abcb1a NNNTCACAAAACTTCACCTG NGG 1 0.6875 Tier II
3 NC_000085.6 19 22180632 - Trpm3 NNNTGGTAAAACTTCACCTG NGG 2 0.4286 Tier II
4 NC_000069.6 3 79684053 - Rxfp1 NNNTCCTAAAACTTCACTTG NGG 2 0.2922 Tier II
5 NC_000076.6 10 119662401 - Grip1 NNNTCATACAACTTCATCTG NGG 2 0.2667 Tier II
6 NC_000074.6 8 54852602 - Gpm6a NNNACATAAAACTTCAACTG NGG 2 0.2222 Tier II
7 NC_000074.6 8 45088508 + Mtnr1a NNNTCAAAAAACTTAACCTG NGG 2 0.1944 Tier II
8 NC_000078.6 12 37910592 + Dgkb NNNTCATAAAGCTTCACCTG NAG 2 0.1685 Tier II
9 NC_000073.6 7 75709747 + Akap13 NNNACATAAAACTTCACCTG NAG 2 0.1235 Tier II
10 NC_000084.6 18 78106333 - Slc14a1 NNNTCATTAAACTTCACCTG NAG 2 0.1111 Tier II
11 NC_000083.6 17 15557547 + Prdm9 NNNTTATAAAACTTCACCTG NGA 2 0.0442 Tier II
12 NC_000075.6 9 78421839 - Ddx43 NNNCCATAAAACTTTACCTG NGG 2 0.0431 Tier II
13 NC_000071.6 5 28328957 + Rbm33 NNNTCATAAGACTTTACCTG NGG 2 0.037 Tier II
14 NC_000079.6 13 105265784 - Rnf180 NNNACATAAAACTTCACCTG NGA 2 0.0331 Tier II
15 NC_000067.6 1 124794233 + Dpp10 NNNGCATAAAACTTCACCTG NTG 2 0.0244 Tier II
16 NC_000072.6 6 18253790 - Cftr NNNTCATAAAACTCTACCTG NGG 2 0.019 Tier II
17 NC_000068.7 2 4493696 - Frmd4a NNNTCATAAAACTTCAGGTG NGG 2 0.0078 Tier II
18 NC_000079.6 13 11895694 - Ryr2 NNNTCATAAAACTTCCCCTG NAG 2 0.0 Tier II
19 NC_000076.6 10 108159234 + Gm40754 NNNGCATAAACCTTCACCTG NGG 2 0.25 Tier III
20 NC_000073.6 7 94620822 + Gm32647 NNNTCCTAAAACTTCACCAG NGG 2 0.1299 Tier III
21 NC_000080.6 14 47822800 - Gm35360 NNNTCATAAACCTCCACCTG NGG 2 0.1143 Tier III
22 NC_000079.6 13 105265784 - Gm52064 NNNACATAAAACTTCACCTG NGA 2 0.0331 Tier III
23 NC_000068.7 2 4493696 - Gm39751 NNNTCATAAAACTTCAGGTG NGG 2 0.0078 Tier III
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Other clones with same target sequence:

(none)