Construct: sgRNA BRDN0001146924
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCTCAAAATCGACACCATTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EPHA8 (2046)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76528
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
22576500 |
+ |
EPHA8 |
NNNCAAAATCGACACCATTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
37761792 |
- |
EPHA10 |
NNNCAAAATCGACACGATCG |
NGG |
2 |
0.0424 |
Tier I |
| 3 |
NC_000002.12 |
2 |
221564112 |
- |
EPHA4 |
NNNCAAAATTGACACCATTG |
NTG |
2 |
0.0367 |
Tier I |
| 4 |
NC_000014.9 |
14 |
37637494 |
+ |
TTC6 |
NNNGAAAATGGACACCATTG |
NGG |
2 |
0.1944 |
Tier II |
| 5 |
NC_000004.12 |
4 |
44250004 |
- |
KCTD8 |
NNNCAAAATCGACACCATTT |
NTG |
2 |
0.0273 |
Tier II |
| 6 |
NC_000003.12 |
3 |
143485265 |
+ |
SLC9A9 |
NNNCAAAATCGCCACCATTG |
NTG |
2 |
0.0103 |
Tier II |
| 7 |
NC_000009.12 |
9 |
22718187 |
+ |
LINC01239 |
NNNTAAAATCTACACCATTG |
NGG |
2 |
0.3077 |
Tier III |
| 8 |
NC_000013.11 |
13 |
76805889 |
+ |
LOC105370265 |
NNNCAAAATGGACACCACTG |
NGG |
2 |
0.1667 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
112462966 |
- |
Sez6l |
NNNCAACATCGGCACCATTG |
NGG |
2 |
0.316 |
Tier I |
| 2 |
NC_000070.6 |
4 |
55016631 |
+ |
Zfp462 |
NNNCAAAATCAACACCACTG |
NGG |
2 |
0.4286 |
Tier II |
| 3 |
NC_000069.6 |
3 |
158388599 |
+ |
Lrrc7 |
NNNCAAAATAGACACTATTG |
NGG |
2 |
0.2667 |
Tier II |
| 4 |
NC_000069.6 |
3 |
102111117 |
- |
Casq2 |
NNNCTAAATGGACACCATTG |
NGG |
2 |
0.1414 |
Tier II |
Other clones with same target sequence:
(none)
