Construct: sgRNA BRDN0001146925
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACAAAATCTCGAATGCCAGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PCK2 (5106)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 78083
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000014.9 |
14 |
24096989 |
- |
PCK2 |
NNNAAATCTCGAATGCCAGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000014.9 |
14 |
24096989 |
- |
NRL |
NNNAAATCTCGAATGCCAGT |
NGG |
0 |
1.0 |
Tier II |
| 3 |
NC_000005.10 |
5 |
58794569 |
+ |
RAB3C |
NNNAAACCTCAAATGCCAGT |
NGG |
2 |
0.6875 |
Tier II |
| 4 |
NC_000011.10 |
11 |
115179634 |
- |
CADM1 |
NNNAAATCTCAAATGCCATT |
NGG |
2 |
0.6667 |
Tier II |
| 5 |
NC_000007.14 |
7 |
158881078 |
+ |
DYNC2I1 |
NNNAAAACGCGAATGCCAGT |
NGG |
2 |
0.5417 |
Tier II |
| 6 |
NC_000004.12 |
4 |
43007220 |
- |
GRXCR1 |
NNNAAATCTCCAATGCCAGA |
NGG |
2 |
0.2411 |
Tier II |
| 7 |
NC_000007.14 |
7 |
28744868 |
+ |
CREB5 |
NNNAAATCTCGAATTCCAGT |
NGG |
1 |
0.1429 |
Tier II |
| 8 |
NC_000003.12 |
3 |
188589569 |
+ |
LPP |
NNNAAAACTCGAATGCCAGT |
NTG |
2 |
0.0341 |
Tier II |
| 9 |
NC_000004.12 |
4 |
183075477 |
+ |
LOC105377578 |
NNNAAATCACGAATGCCAGT |
NGG |
1 |
0.9286 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000080.6 |
14 |
98123549 |
+ |
Dach1 |
NNNAAATCCCGAATGCCATT |
NGG |
2 |
0.6154 |
Tier II |
| 2 |
NC_000071.6 |
5 |
25295221 |
+ |
Kmt2c |
NNNAAATCTCCAATGCCAAT |
NGG |
2 |
0.3061 |
Tier II |
| 3 |
NC_000076.6 |
10 |
110085437 |
- |
Nav3 |
NNNAAATCTCAAATGCCAGG |
NGG |
2 |
0.1765 |
Tier II |
| 4 |
NC_000086.7 |
X |
135865025 |
- |
Bhlhb9 |
NNNAAATCTCAAATGGCAGT |
NGG |
2 |
0.1538 |
Tier II |
| 5 |
NC_000082.6 |
16 |
72658042 |
- |
Robo1 |
NNNAAATCTCCAAGGCCAGT |
NGG |
2 |
0.0 |
Tier II |
| 6 |
NC_000069.6 |
3 |
87292435 |
- |
Gm31879 |
NNNAAATGTCTAATGCCAGT |
NGG |
2 |
0.2473 |
Tier III |
| 7 |
NC_000082.6 |
16 |
72658042 |
- |
Gm29908 |
NNNAAATCTCCAAGGCCAGT |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
