Construct: sgRNA BRDN0001146928
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCTGGAGAGACAATTCTACT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80252
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
116058048 |
+ |
LSAMP |
NNNGGAAAGAAAATTCTACT |
NGG |
2 |
0.75 |
Tier II |
| 2 |
NC_000006.12 |
6 |
30573434 |
+ |
ABCF1 |
NNNGGAGAGACAACTATACT |
NGG |
2 |
0.2857 |
Tier II |
| 3 |
NC_000005.10 |
5 |
60884683 |
+ |
ERCC8 |
NNNGGACAGACTATTCTACT |
NGG |
2 |
0.2292 |
Tier II |
| 4 |
NC_000012.12 |
12 |
14630729 |
+ |
GUCY2C |
NNNGGAGAGACGACTCTACT |
NGG |
2 |
0.2063 |
Tier II |
| 5 |
NC_000006.12 |
6 |
45884499 |
+ |
CLIC5 |
NNNGGAAAGACAATTCTCCT |
NGG |
2 |
0.1905 |
Tier II |
| 6 |
NC_000009.12 |
9 |
124396571 |
+ |
PSMB7 |
NNNGGAGAGAGAATTCTACC |
NGG |
2 |
0.0227 |
Tier II |
| 7 |
NC_000012.12 |
12 |
40393394 |
- |
MUC19 |
NNNGGAGAGACAAGTCTACA |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000003.12 |
3 |
116058048 |
+ |
LOC124906269 |
NNNGGAAAGAAAATTCTACT |
NGG |
2 |
0.75 |
Tier III |
| 9 |
NC_000013.11 |
13 |
78554785 |
- |
OBI1-AS1 |
NNNAGAGAGACAATTCTCCT |
NGG |
2 |
0.1714 |
Tier III |
| 10 |
NC_000009.12 |
9 |
135164695 |
- |
LOC102723948 |
NNNGGAGAGACAATTCCACC |
NGG |
2 |
0.0642 |
Tier III |
| 11 |
NC_000006.12 |
6 |
122994763 |
- |
LOC124901392 |
NNNGGAGAGACAATTCTCCC |
NGG |
2 |
0.0173 |
Tier III |
| 12 |
NC_000002.12 |
2 |
13851055 |
+ |
LOC107985854 |
NNNGGAGAGACTATTCTACT |
NGT |
2 |
0.0054 |
Tier III |
| 13 |
NC_000012.12 |
12 |
40393394 |
- |
LOC105369736 |
NNNGGAGAGACAAGTCTACA |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
71179779 |
+ |
Nlrp1b |
NNNGGAGAGACAATTCTATT |
NGG |
1 |
0.4615 |
Tier II |
| 2 |
NC_000078.6 |
12 |
108638623 |
+ |
Evl |
NNNGGAGAGACAGTTCTTCT |
NGG |
2 |
0.3261 |
Tier II |
| 3 |
NC_000074.6 |
8 |
81424857 |
+ |
Inpp4b |
NNNGGATAGACAATTCTACA |
NGG |
2 |
0.3214 |
Tier II |
| 4 |
NC_000071.6 |
5 |
13570843 |
- |
Sema3a |
NNNGGGGAGACAATTTTACT |
NGG |
2 |
0.2198 |
Tier II |
| 5 |
NC_000067.6 |
1 |
84647919 |
- |
Dner |
NNNGGAGAGGCAATTTTACT |
NGG |
2 |
0.1709 |
Tier II |
| 6 |
NC_000077.6 |
11 |
115055701 |
+ |
Cd300e |
NNNGGAGAGACATTTGTACT |
NGG |
2 |
0.0462 |
Tier II |
| 7 |
NC_000067.6 |
1 |
125800136 |
- |
Gpr39 |
NNNGGAGAGACACTTCTCCT |
NGG |
2 |
0.0401 |
Tier II |
| 8 |
NC_000074.6 |
8 |
66435758 |
- |
Marchf1 |
NNNGGAGAGACAATTCTACT |
NCA |
2 |
0.0 |
Tier II |
| 9 |
NC_000067.6 |
1 |
64715634 |
+ |
Ccnyl1 |
NNNTGAGAGACAAGTCTACT |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000081.6 |
15 |
35268719 |
- |
Gm33497 |
NNNGAAGAGAGAATTCTACT |
NGG |
2 |
0.2167 |
Tier III |
| 11 |
NC_000081.6 |
15 |
35268719 |
- |
Gm52178 |
NNNGAAGAGAGAATTCTACT |
NGG |
2 |
0.2167 |
Tier III |
| 12 |
NC_000086.7 |
X |
74007345 |
+ |
Gm8545 |
NNNGGAGAGAGACTTCTACT |
NGG |
2 |
0.0526 |
Tier III |
Other clones with same target sequence:
(none)
