Construct: sgRNA BRDN0001146930

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TACAACTCTGATGTTCTAGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IRAK3 (11213)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77714

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000012.12 12 66244561 - IRAK3 NNNAACTCTGATGTTCTAGG NGG 0 1.0 Tier I
2 NC_000009.12 9 130302409 + HMCN2 NNNAAAACTGATGTTCTAGG NGG 2 0.8125 Tier II
3 NC_000010.11 10 132310537 + STK32C NNNAGCTCTGAAGTTCTAGG NGG 2 0.576 Tier II
4 NC_000004.12 4 112969116 + ANK2 NNNAACACTGAGGTTCTAGG NGG 2 0.4375 Tier II
5 NC_000013.11 13 107795475 - NALF1 NNNAACTGTGAGGTTCTAGG NGG 2 0.3214 Tier II
6 NC_000010.11 10 1652125 + ADARB2 NNNATCTTTGATGTTCTAGG NGG 2 0.3182 Tier II
7 NC_000023.11 X 132889741 + HS6ST2 NNNAGCTCTGATGTTCTGGG NGG 2 0.288 Tier II
8 NC_000002.12 2 100042808 - AFF3 NNNAACTCTGATGTTCTAGG NAG 1 0.2593 Tier II
9 NC_000010.11 10 119530286 + RGS10 NNNATCTCTGATGTTCTATG NGG 2 0.2424 Tier II
10 NC_000002.12 2 25238061 - DNMT3A NNNAACTCTGAGCTTCTAGG NGG 2 0.2105 Tier II
11 NC_000003.12 3 121323397 + STXBP5L NNNAACTCTGATTTTCTATG NGG 2 0.2 Tier II
12 NC_000010.11 10 80624774 + SH2D4B NNNGACTCTGTTGTTCTAGG NGG 2 0.1923 Tier II
13 NC_000017.11 17 12726294 + MYOCD NNNAACTCTGATGTTGTAGC NGG 2 0.0659 Tier II
14 NC_000007.14 7 11458101 + THSD7A NNNAATTCTGATGTGCTAGG NGG 2 0.0464 Tier II
15 NC_000002.12 2 222905584 + ACSL3 NNNAACTCTGATGTTCTAGG NGT 1 0.0161 Tier II
16 NC_000005.10 5 127017500 - MARCHF3 NNNAACTATGATGTTCTAGG NGT 2 0.0105 Tier II
17 NC_000018.10 18 46553435 - LOXHD1 NNNAACCCTGATGGTCTAGG NGG 2 0.0 Tier II
18 NC_000007.14 7 130930479 + LINC-PINT NNNAACTCAGATGTTCTAGA NGG 2 0.8705 Tier III
19 NC_000013.11 13 107795475 - NALF1-IT1 NNNAACTGTGAGGTTCTAGG NGG 2 0.3214 Tier III
20 NC_000005.10 5 159749633 + LOC105377688 NNNAACTCTGATGTTGGAGG NGG 2 0.0181 Tier III
21 NC_000001.11 1 38360609 - LOC105378657 NNNAACTCTGATGTTCTAGG NAT 2 0.0 Tier III
22 NC_000018.10 18 73194835 - LINC02864 NNNAACTCTGATGTTCTAGG NAA 2 0.0 Tier III
23 NC_000024.10 Y 18981956 + TTTY14 NNNAACTCTGATGTTCTAGG NCA 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 79683811 - Rab11fip4 NNNAACACTGAGGTTCTAGG NGG 2 0.4375 Tier II
2 NC_000067.6 1 171595625 + Ly9 NNNAGCTCTGATGTTCAAGG NGG 2 0.384 Tier II
3 NC_000067.6 1 165246684 + Tiprl NNNAACTCACATGTTCTAGG NGG 2 0.3714 Tier II
4 NC_000070.6 4 21889142 - Coq3 NNNAACTCTGAAGTTCTAGC NGG 2 0.3429 Tier II
5 NC_000067.6 1 135991427 + Igfn1 NNNAACTGTGATGTTCAAGG NGG 2 0.3429 Tier II
6 NC_000084.6 18 47280898 + Sema6a NNNAACTGTGATGTTCAAGG NGG 2 0.3429 Tier II
7 NC_000073.6 7 120433933 + Abca16 NNNAACTCTAATTTTCTAGG NGG 2 0.28 Tier II
8 NC_000074.6 8 10584847 - Myo16 NNNAACTGTGATCTTCTAGG NGG 2 0.2707 Tier II
9 NC_000077.6 11 46973687 + Sgcd NNNGACTCTGATGTCCTAGG NGG 2 0.1705 Tier II
10 NC_000083.6 17 34242194 - H2-Ob NNNAACTCTGAGGTTTTAGG NGG 2 0.1538 Tier II
11 NC_000067.6 1 153107803 - Nmnat2 NNNATCTCTGCTGTTCTAGG NGG 2 0.1455 Tier II
12 NC_000067.6 1 152577521 + Rgl1 NNNCACTCTGATGTTTTAGG NGG 2 0.1086 Tier II
13 NC_000073.6 7 114782541 - Insc NNNAACTCTGATATTCTAGG NGC 2 0.0205 Tier II
14 NC_000069.6 3 116337922 - Cdc14a NNNAACTCTGATGTTCTAGG NCC 2 0.0 Tier II
15 NC_000076.6 10 66406963 - Gm40677 NNNAATTCTGATGTCCTAGG NGG 2 0.2532 Tier III
16 NC_000070.6 4 114848145 + Gm12830 NNNAACTCTCATGGTCTAGG NGG 2 0.0 Tier III
17 NC_000070.6 4 114848145 + Gm33655 NNNAACTCTCATGGTCTAGG NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)