Construct: sgRNA BRDN0001146930
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TACAACTCTGATGTTCTAGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- IRAK3 (11213)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77714
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000012.12 |
12 |
66244561 |
- |
IRAK3 |
NNNAACTCTGATGTTCTAGG |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000009.12 |
9 |
130302409 |
+ |
HMCN2 |
NNNAAAACTGATGTTCTAGG |
NGG |
2 |
0.8125 |
Tier II |
3 |
NC_000010.11 |
10 |
132310537 |
+ |
STK32C |
NNNAGCTCTGAAGTTCTAGG |
NGG |
2 |
0.576 |
Tier II |
4 |
NC_000004.12 |
4 |
112969116 |
+ |
ANK2 |
NNNAACACTGAGGTTCTAGG |
NGG |
2 |
0.4375 |
Tier II |
5 |
NC_000013.11 |
13 |
107795475 |
- |
NALF1 |
NNNAACTGTGAGGTTCTAGG |
NGG |
2 |
0.3214 |
Tier II |
6 |
NC_000010.11 |
10 |
1652125 |
+ |
ADARB2 |
NNNATCTTTGATGTTCTAGG |
NGG |
2 |
0.3182 |
Tier II |
7 |
NC_000023.11 |
X |
132889741 |
+ |
HS6ST2 |
NNNAGCTCTGATGTTCTGGG |
NGG |
2 |
0.288 |
Tier II |
8 |
NC_000002.12 |
2 |
100042808 |
- |
AFF3 |
NNNAACTCTGATGTTCTAGG |
NAG |
1 |
0.2593 |
Tier II |
9 |
NC_000010.11 |
10 |
119530286 |
+ |
RGS10 |
NNNATCTCTGATGTTCTATG |
NGG |
2 |
0.2424 |
Tier II |
10 |
NC_000002.12 |
2 |
25238061 |
- |
DNMT3A |
NNNAACTCTGAGCTTCTAGG |
NGG |
2 |
0.2105 |
Tier II |
11 |
NC_000003.12 |
3 |
121323397 |
+ |
STXBP5L |
NNNAACTCTGATTTTCTATG |
NGG |
2 |
0.2 |
Tier II |
12 |
NC_000010.11 |
10 |
80624774 |
+ |
SH2D4B |
NNNGACTCTGTTGTTCTAGG |
NGG |
2 |
0.1923 |
Tier II |
13 |
NC_000017.11 |
17 |
12726294 |
+ |
MYOCD |
NNNAACTCTGATGTTGTAGC |
NGG |
2 |
0.0659 |
Tier II |
14 |
NC_000007.14 |
7 |
11458101 |
+ |
THSD7A |
NNNAATTCTGATGTGCTAGG |
NGG |
2 |
0.0464 |
Tier II |
15 |
NC_000002.12 |
2 |
222905584 |
+ |
ACSL3 |
NNNAACTCTGATGTTCTAGG |
NGT |
1 |
0.0161 |
Tier II |
16 |
NC_000005.10 |
5 |
127017500 |
- |
MARCHF3 |
NNNAACTATGATGTTCTAGG |
NGT |
2 |
0.0105 |
Tier II |
17 |
NC_000018.10 |
18 |
46553435 |
- |
LOXHD1 |
NNNAACCCTGATGGTCTAGG |
NGG |
2 |
0.0 |
Tier II |
18 |
NC_000007.14 |
7 |
130930479 |
+ |
LINC-PINT |
NNNAACTCAGATGTTCTAGA |
NGG |
2 |
0.8705 |
Tier III |
19 |
NC_000013.11 |
13 |
107795475 |
- |
NALF1-IT1 |
NNNAACTGTGAGGTTCTAGG |
NGG |
2 |
0.3214 |
Tier III |
20 |
NC_000005.10 |
5 |
159749633 |
+ |
LOC105377688 |
NNNAACTCTGATGTTGGAGG |
NGG |
2 |
0.0181 |
Tier III |
21 |
NC_000001.11 |
1 |
38360609 |
- |
LOC105378657 |
NNNAACTCTGATGTTCTAGG |
NAT |
2 |
0.0 |
Tier III |
22 |
NC_000018.10 |
18 |
73194835 |
- |
LINC02864 |
NNNAACTCTGATGTTCTAGG |
NAA |
2 |
0.0 |
Tier III |
23 |
NC_000024.10 |
Y |
18981956 |
+ |
TTTY14 |
NNNAACTCTGATGTTCTAGG |
NCA |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000077.6 |
11 |
79683811 |
- |
Rab11fip4 |
NNNAACACTGAGGTTCTAGG |
NGG |
2 |
0.4375 |
Tier II |
2 |
NC_000067.6 |
1 |
171595625 |
+ |
Ly9 |
NNNAGCTCTGATGTTCAAGG |
NGG |
2 |
0.384 |
Tier II |
3 |
NC_000067.6 |
1 |
165246684 |
+ |
Tiprl |
NNNAACTCACATGTTCTAGG |
NGG |
2 |
0.3714 |
Tier II |
4 |
NC_000070.6 |
4 |
21889142 |
- |
Coq3 |
NNNAACTCTGAAGTTCTAGC |
NGG |
2 |
0.3429 |
Tier II |
5 |
NC_000067.6 |
1 |
135991427 |
+ |
Igfn1 |
NNNAACTGTGATGTTCAAGG |
NGG |
2 |
0.3429 |
Tier II |
6 |
NC_000084.6 |
18 |
47280898 |
+ |
Sema6a |
NNNAACTGTGATGTTCAAGG |
NGG |
2 |
0.3429 |
Tier II |
7 |
NC_000073.6 |
7 |
120433933 |
+ |
Abca16 |
NNNAACTCTAATTTTCTAGG |
NGG |
2 |
0.28 |
Tier II |
8 |
NC_000074.6 |
8 |
10584847 |
- |
Myo16 |
NNNAACTGTGATCTTCTAGG |
NGG |
2 |
0.2707 |
Tier II |
9 |
NC_000077.6 |
11 |
46973687 |
+ |
Sgcd |
NNNGACTCTGATGTCCTAGG |
NGG |
2 |
0.1705 |
Tier II |
10 |
NC_000083.6 |
17 |
34242194 |
- |
H2-Ob |
NNNAACTCTGAGGTTTTAGG |
NGG |
2 |
0.1538 |
Tier II |
11 |
NC_000067.6 |
1 |
153107803 |
- |
Nmnat2 |
NNNATCTCTGCTGTTCTAGG |
NGG |
2 |
0.1455 |
Tier II |
12 |
NC_000067.6 |
1 |
152577521 |
+ |
Rgl1 |
NNNCACTCTGATGTTTTAGG |
NGG |
2 |
0.1086 |
Tier II |
13 |
NC_000073.6 |
7 |
114782541 |
- |
Insc |
NNNAACTCTGATATTCTAGG |
NGC |
2 |
0.0205 |
Tier II |
14 |
NC_000069.6 |
3 |
116337922 |
- |
Cdc14a |
NNNAACTCTGATGTTCTAGG |
NCC |
2 |
0.0 |
Tier II |
15 |
NC_000076.6 |
10 |
66406963 |
- |
Gm40677 |
NNNAATTCTGATGTCCTAGG |
NGG |
2 |
0.2532 |
Tier III |
16 |
NC_000070.6 |
4 |
114848145 |
+ |
Gm12830 |
NNNAACTCTCATGGTCTAGG |
NGG |
2 |
0.0 |
Tier III |
17 |
NC_000070.6 |
4 |
114848145 |
+ |
Gm33655 |
NNNAACTCTCATGGTCTAGG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)