Construct: sgRNA BRDN0001146931

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ATCCTGAGACTTGTTACCAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TK2 (7084)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77298

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000016.10 16 66517184 - TK2 NNNCTGAGACTTGTTACCAG NGG 0 1.0 Tier I
2 NC_000023.11 X 107062434 - RBM41 NNNCTGATACATGTTACCAG NGG 2 0.4688 Tier II
3 NC_000009.12 9 13131625 - MPDZ NNNCTGAAACTTGTTATCAG NGG 2 0.4667 Tier II
4 NC_000012.12 12 125285097 - TMEM132B NNNCTGAGACTTGTTACTAT NGG 2 0.45 Tier II
5 NC_000011.10 11 89057637 - GRM5 NNNCTGAGACTAGTTAACAG NGG 2 0.3733 Tier II
6 NC_000003.12 3 49003157 - P4HTM NNNCTGAGAGTTATTACCAG NGG 2 0.359 Tier II
7 NC_000011.10 11 2736430 + KCNQ1 NNNCTGAGACTTGTGACCAG NGG 1 0.05 Tier II
8 NC_000001.11 1 201611070 - NAV1 NNNCTGGGACTTGTTAGCAG NGG 2 0.0415 Tier II
9 NC_000002.12 2 109135434 + RANBP2 NNNCTGAGACTTGTTATCAG NGA 2 0.0324 Tier II
10 NC_000002.12 2 109135434 + SH3RF3 NNNCTGAGACTTGTTATCAG NGA 2 0.0324 Tier II
11 NC_000007.14 7 150102204 + ACTR3C NNNCTGAGCCTTGGTACCAG NGG 2 0.0 Tier II
12 NC_000012.12 12 125285097 - LOC107984445 NNNCTGAGACTTGTTACTAT NGG 2 0.45 Tier III
13 NC_000002.12 2 184150335 - LOC105373777 NNNCAGAGAATTGTTACCAG NGG 2 0.4333 Tier III
14 NC_000011.10 11 49566859 + GRM5P1 NNNCTGAGACTAGTTAACAG NGG 2 0.3733 Tier III
15 NC_000021.9 21 26442431 + CYYR1-AS1 NNNGTGAGAGTTGTTACCAG NGG 2 0.1944 Tier III
16 NC_000003.12 3 122891703 + LINC02035 NNNCTGAGACTTGATGCCAG NGG 2 0.119 Tier III
17 NC_000001.11 1 201611070 - LOC124904482 NNNCTGGGACTTGTTAGCAG NGG 2 0.0415 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 96982390 - Lrrc4c NNNCTGATACTTGTTACCAA NGG 2 0.5859 Tier II
2 NC_000082.6 16 59977400 - Epha6 NNNCTGAGACTTGTTAACAG NGG 1 0.4667 Tier II
3 NC_000075.6 9 63141467 - Skor1 NNNCTGGGACTTGTAACCAG NGG 2 0.4087 Tier II
4 NC_000078.6 12 108831389 - Slc25a29 NNNCTGACTCTTGTTACCAG NGG 2 0.3692 Tier II
5 NC_000076.6 10 95291581 - Cradd NNNGTCAGACTTGTTACCAG NGG 2 0.3409 Tier II
6 NC_000078.6 12 116208544 - Wdr60 NNNCTGAGACTTGTTACTGG NGG 2 0.2411 Tier II
7 NC_000070.6 4 148896775 - Casz1 NNNCAGAGACTTGTTAACAG NGG 2 0.2333 Tier II
8 NC_000084.6 18 61344320 - Ppargc1b NNNCTGGGACTTGTCACCAG NGG 2 0.1925 Tier II
9 NC_000071.6 5 124853731 - Ccdc92 NNNCTGTGACTTCTTACCAG NGG 2 0.1842 Tier II
10 NC_000076.6 10 122865301 + Ppm1h NNNCTCAGACTTGTGACCAG NGG 2 0.0341 Tier II
11 NC_000085.6 19 55388207 + Vti1a NNNCTGAGACTTGTGAACAG NGG 2 0.0233 Tier II
12 NC_000073.6 7 115628982 - Sox6 NNNCTGTGACTTGTGACCAG NGG 2 0.0219 Tier II
13 NC_000073.6 7 78568369 - Ntrk3 NNNCTGTGACTTGTTACCAG NGC 2 0.0097 Tier II
14 NC_000071.6 5 27050665 + Dpp6 NNNCTGAGACTTGTTCCCAG NGA 2 0.0 Tier II
15 NC_000072.6 6 145049390 + Bcat1 NNNCTGAGACTTGTTTCCAA NGG 2 0.0 Tier II
16 NC_000076.6 10 90753755 - Anks1b NNNCTGAGACTTGTTTGCAG NGG 2 0.0 Tier II
17 NC_000086.7 X 92520241 - Mageb18 NNNCTGAGACTTGTTTTCAG NGG 2 0.0 Tier II
18 NC_000075.6 9 41513079 + Mir100hg NNNCTGAGACTTGTTACCTA NGG 2 0.5048 Tier III
19 NC_000081.6 15 79196797 + Gm10863 NNNCTGAGGCTTGTTACCAG NGA 2 0.0463 Tier III
20 NC_000083.6 17 78460605 + Gm32464 NNNCTGAGACTTGTTACCTG NTG 2 0.021 Tier III
21 NC_000073.6 7 78568369 - Gm39038 NNNCTGTGACTTGTTACCAG NGC 2 0.0097 Tier III
22 NC_000072.6 6 145049390 + Gm26666 NNNCTGAGACTTGTTTCCAA NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)