Construct: sgRNA BRDN0001146934
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACATTGTTGGTGCAACGAGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAGI1 (9223)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 78069
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
65429525 |
- |
MAGI1 |
NNNTTGTTGGTGCAACGAGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
47107542 |
- |
CYP4Z1 |
NNNTTGTTGGAGCAACTAGG |
NGG |
2 |
0.1875 |
Tier II |
| 3 |
NC_000002.12 |
2 |
72146405 |
- |
CYP26B1 |
NNNTTGTTCGTGCAATGAGG |
NGG |
2 |
0.1657 |
Tier II |
| 4 |
NC_000001.11 |
1 |
24452235 |
+ |
NIPAL3 |
NNNTTGTTGATGCAAGGAGG |
NGG |
2 |
0.1436 |
Tier II |
| 5 |
NC_000020.11 |
20 |
42095196 |
- |
PTPRT |
NNNTTGTTGGTGCAAGGATG |
NGG |
2 |
0.1026 |
Tier II |
| 6 |
NC_000002.12 |
2 |
3544558 |
+ |
RNASEH1 |
NNNTTGTTGGGGCAAGGAGG |
NGG |
2 |
0.0615 |
Tier II |
| 7 |
NC_000005.10 |
5 |
135101388 |
+ |
PITX1-AS1 |
NNNTTGTTGATGCAATGAGG |
NGG |
2 |
0.2872 |
Tier III |
| 8 |
NC_000020.11 |
20 |
42095196 |
- |
LOC101927182 |
NNNTTGTTGGTGCAAGGATG |
NGG |
2 |
0.1026 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
93745439 |
- |
Magi1 |
NNNCTGTTGGTGCAGCGAGG |
NGG |
2 |
0.4206 |
Tier I |
| 2 |
NC_000075.6 |
9 |
43773878 |
- |
Nectin1 |
NNNTTGTGGGTGCAAAGAGG |
NGG |
2 |
0.7333 |
Tier II |
| 3 |
NC_000067.6 |
1 |
57871509 |
+ |
Spats2l |
NNNTTGGTGGTGCAAAGAGG |
NGG |
2 |
0.5882 |
Tier II |
| 4 |
NC_000078.6 |
12 |
53851761 |
- |
Npas3 |
NNNTTTTTGGTTCAACGAGG |
NGG |
2 |
0.2564 |
Tier II |
| 5 |
NC_000075.6 |
9 |
43773878 |
- |
Gm46108 |
NNNTTGTGGGTGCAAAGAGG |
NGG |
2 |
0.7333 |
Tier III |
| 6 |
NC_000069.6 |
3 |
70802008 |
+ |
Gm6634 |
NNNTTGTTGGTGCAAAGAGG |
NAG |
2 |
0.2593 |
Tier III |
| 7 |
NC_000078.6 |
12 |
53851761 |
- |
Gm35614 |
NNNTTTTTGGTTCAACGAGG |
NGG |
2 |
0.2564 |
Tier III |
Other clones with same target sequence:
(none)
