Construct: sgRNA BRDN0001146939
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAAGGATATTACAGCCGAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PANK1 (53354)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76556
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
89611971 |
- |
PANK1 |
NNNGGATATTACAGCCGAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
139471916 |
+ |
KLRG2 |
NNNGGATATGACAGCCAAAG |
NGG |
2 |
0.4667 |
Tier II |
| 3 |
NC_000007.14 |
7 |
28773470 |
+ |
CREB5 |
NNNGTATATTACAGCAGAAG |
NGG |
2 |
0.3 |
Tier II |
| 4 |
NC_000022.11 |
22 |
44016670 |
+ |
PARVB |
NNNAGATATTACAGCTGAAG |
NGG |
2 |
0.2769 |
Tier II |
| 5 |
NC_000004.12 |
4 |
26822561 |
+ |
TBC1D19 |
NNNGGTTATTACAGCCCAAG |
NGG |
2 |
0.1681 |
Tier II |
| 6 |
NC_000005.10 |
5 |
43544801 |
- |
PAIP1 |
NNNGTATATTACAGCTGAAG |
NGG |
2 |
0.0923 |
Tier II |
| 7 |
NC_000009.12 |
9 |
19054231 |
+ |
HAUS6 |
NNNGGAGATTACAGCGGAAG |
NGG |
2 |
0.0905 |
Tier II |
| 8 |
NC_000007.14 |
7 |
53865785 |
- |
HAUS6P3 |
NNNGGAGATTACAGCGGAAG |
NGG |
2 |
0.0905 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
132528915 |
+ |
Cntn2 |
NNNGGATCTCACAGCCGAAG |
NGG |
2 |
0.2286 |
Tier I |
| 2 |
NC_000071.6 |
5 |
26963035 |
+ |
Dpp6 |
NNNGGATATTAGAGCCAAAG |
NGG |
2 |
0.4148 |
Tier II |
| 3 |
NC_000086.7 |
X |
94123483 |
- |
Zfx |
NNNGGATTTTACAGCCGAGG |
NGG |
2 |
0.1607 |
Tier II |
| 4 |
NC_000067.6 |
1 |
163263383 |
- |
Prrx1 |
NNNTGATATTACAGCTGAAG |
NGG |
2 |
0.1119 |
Tier II |
| 5 |
NC_000078.6 |
12 |
64994460 |
- |
Togaram1 |
NNNGGATATTACAGCCCAAG |
NTG |
2 |
0.0092 |
Tier II |
| 6 |
NC_000080.6 |
14 |
90134149 |
+ |
4930474H20Rik |
NNNGGATATTCCAGCAGAAG |
NGG |
2 |
0.4 |
Tier III |
Other clones with same target sequence:
(none)
