Construct: sgRNA BRDN0001146940
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTGGAGACCTCTTCAATGGC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SRPK2 (6733)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75601
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
105169228 |
- |
SRPK2 |
NNNGAGACCTCTTCAATGGC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000016.10 |
16 |
70160484 |
- |
PDPR |
NNNGAGATCTCTTCAATGTC |
NGG |
2 |
0.5833 |
Tier II |
| 3 |
NC_000008.11 |
8 |
62863821 |
- |
NKAIN3 |
NNNCAGACCTCTTCAATGGC |
NGG |
1 |
0.5294 |
Tier II |
| 4 |
NC_000015.10 |
15 |
57120485 |
- |
TCF12 |
NNNGAGAGCTCATCAATGGC |
NGG |
2 |
0.5143 |
Tier II |
| 5 |
NC_000007.14 |
7 |
45158817 |
- |
RAMP3 |
NNNAAGACCTCTTCAATGGA |
NGG |
2 |
0.45 |
Tier II |
| 6 |
NC_000016.10 |
16 |
78977243 |
+ |
WWOX |
NNNGAGAGCTCTTCAAAGGC |
NGG |
2 |
0.3429 |
Tier II |
| 7 |
NC_000023.11 |
X |
19501728 |
- |
MAP3K15 |
NNNGCGAACTCTTCAATGGC |
NGG |
2 |
0.325 |
Tier II |
| 8 |
NC_000023.11 |
X |
19704718 |
- |
SH3KBP1 |
NNNGAGAGCTTTTCAATGGC |
NGG |
2 |
0.1978 |
Tier II |
| 9 |
NC_000003.12 |
3 |
159595500 |
- |
SCHIP1 |
NNNGAGACCTCTTCAGAGGC |
NGG |
2 |
0.1026 |
Tier II |
| 10 |
NC_000003.12 |
3 |
159595500 |
- |
IQCJ-SCHIP1 |
NNNGAGACCTCTTCAGAGGC |
NGG |
2 |
0.1026 |
Tier II |
| 11 |
NC_000019.10 |
19 |
39136923 |
- |
PAK4 |
NNNGAGACCTCTGCAATGGC |
NGC |
2 |
0.0058 |
Tier II |
| 12 |
NC_000010.11 |
10 |
127373380 |
+ |
DOCK1 |
NNNGAGAACTCTTCACTGGC |
NGG |
2 |
0.0 |
Tier II |
| 13 |
NC_000001.11 |
1 |
17516791 |
- |
ARHGEF10L |
NNNGAGACCTCTTCGTTGGC |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000016.10 |
16 |
70160484 |
- |
LOC400541 |
NNNGAGATCTCTTCAATGTC |
NGG |
2 |
0.5833 |
Tier III |
| 15 |
NC_000008.11 |
8 |
62863821 |
- |
SRPK2P |
NNNCAGACCTCTTCAATGGC |
NGG |
1 |
0.5294 |
Tier III |
| 16 |
NC_000008.11 |
8 |
62863821 |
- |
LOC112268019 |
NNNCAGACCTCTTCAATGGC |
NGG |
1 |
0.5294 |
Tier III |
| 17 |
NC_000001.11 |
1 |
194081056 |
- |
LOC124904475 |
NNNCAGACCTCATCAATGGC |
NGG |
2 |
0.4235 |
Tier III |
| 18 |
NC_000015.10 |
15 |
82659081 |
- |
CPEB1-AS1 |
NNNAAGTCCTCTTCAATGGC |
NGG |
2 |
0.3938 |
Tier III |
| 19 |
NC_000002.12 |
2 |
47201015 |
- |
EPCAM-DT |
NNNGAGAGCTCTTCAAGGGC |
NGG |
2 |
0.0756 |
Tier III |
| 20 |
NC_000010.11 |
10 |
127373380 |
+ |
LOC105378551 |
NNNGAGAACTCTTCACTGGC |
NGG |
2 |
0.0 |
Tier III |
| 21 |
NC_000002.12 |
2 |
65792649 |
+ |
LINC02934 |
NNNGAGACCTCTTCATAGGC |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
130977454 |
- |
Htra1 |
NNNGAGACCTCTACAATGGC |
NGG |
1 |
0.6923 |
Tier II |
| 2 |
NC_000072.6 |
6 |
85903211 |
+ |
Nat8f4 |
NNNGAGACCTCTCCAAAGGC |
NGG |
2 |
0.4211 |
Tier II |
| 3 |
NC_000081.6 |
15 |
95639070 |
+ |
Dbx2 |
NNNGAGACCTCTTCAATAGA |
NGG |
2 |
0.3462 |
Tier II |
| 4 |
NC_000079.6 |
13 |
24993857 |
- |
Mrs2 |
NNNGAGCACTCTTCAATGGC |
NGG |
2 |
0.2844 |
Tier II |
| 5 |
NC_000075.6 |
9 |
119014168 |
- |
Ctdspl |
NNNGTGAGCTCTTCAATGGC |
NGG |
2 |
0.2338 |
Tier II |
| 6 |
NC_000071.6 |
5 |
96524177 |
+ |
Fras1 |
NNNGAGACCTCTTCCATGGT |
NGG |
2 |
0.0818 |
Tier II |
| 7 |
NC_000077.6 |
11 |
30238256 |
- |
Sptbn1 |
NNNGAGACCTGTTCAAGGGC |
NGG |
2 |
0.0294 |
Tier II |
| 8 |
NC_000080.6 |
14 |
86722865 |
+ |
Diaph3 |
NNNGAGACCTTTTCAATGGG |
NGG |
2 |
0.0181 |
Tier II |
| 9 |
NC_000085.6 |
19 |
40964185 |
- |
Blnk |
NNNGAGACCTCTTCAATGGC |
NTC |
2 |
0.0 |
Tier II |
| 10 |
NC_000067.6 |
1 |
118531844 |
+ |
Clasp1 |
NNNGAGACCTCTTCATGGGC |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000068.7 |
2 |
93414151 |
+ |
Gm13807 |
NNNGAGACCTCTTCAATGGC |
NTC |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
