Construct: sgRNA BRDN0001146941
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACACCACCTGAAGTGACTCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CHEK1 (1111)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76641
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
125643809 |
- |
CHEK1 |
NNNCCACCTGAAGTGACTCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000018.10 |
18 |
6037029 |
- |
L3MBTL4 |
NNNCCAACTGAAGTGACTCA |
NGG |
2 |
0.7031 |
Tier II |
| 3 |
NC_000019.10 |
19 |
19321556 |
+ |
MAU2 |
NNNCCACCTAAAGTGACTTG |
NGG |
2 |
0.4308 |
Tier II |
| 4 |
NC_000011.10 |
11 |
17353031 |
- |
NCR3LG1 |
NNNCCACCTGCAGTGACTCG |
NGG |
1 |
0.4 |
Tier II |
| 5 |
NC_000002.12 |
2 |
205510463 |
- |
PARD3B |
NNNCCACCTGAAGTGACCCT |
NGG |
2 |
0.3 |
Tier II |
| 6 |
NC_000003.12 |
3 |
136105260 |
- |
PPP2R3A |
NNNCCACCTGGAGTGACTTG |
NGG |
2 |
0.3 |
Tier II |
| 7 |
NC_000022.11 |
22 |
29830392 |
+ |
ASCC2 |
NNNCCACCTCAAGTGACTTG |
NGG |
2 |
0.1846 |
Tier II |
| 8 |
NC_000017.11 |
17 |
17099985 |
+ |
MPRIP |
NNNCCACCTGATGTCACTCG |
NGG |
2 |
0.0909 |
Tier II |
| 9 |
NC_000001.11 |
1 |
248499629 |
- |
LOC105373277 |
NNNCCACCTGCACTGACTCG |
NGG |
2 |
0.1684 |
Tier III |
| 10 |
NC_000001.11 |
1 |
166237522 |
+ |
LOC112268276 |
NNNCCAACTGAAGTGGCTCG |
NGG |
2 |
0.1442 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
41703849 |
+ |
Kel |
NNNCCTCCTGAAGTGACTAG |
NGG |
2 |
0.3061 |
Tier II |
| 2 |
NC_000082.6 |
16 |
55973792 |
- |
Zbtb11 |
NNNCCACCGGAAGTGACGCG |
NGG |
2 |
0.2063 |
Tier II |
| 3 |
NC_000075.6 |
9 |
105883254 |
- |
Col6a5 |
NNNCCACCTAAAGTGACTGG |
NGG |
2 |
0.1167 |
Tier II |
| 4 |
NC_000082.6 |
16 |
55973792 |
- |
Zbtb11os1 |
NNNCCACCGGAAGTGACGCG |
NGG |
2 |
0.2063 |
Tier III |
| 5 |
NC_000083.6 |
17 |
15884278 |
+ |
Gm41533 |
NNNCCACCTGAATTGACTCC |
NGG |
2 |
0.1286 |
Tier III |
Other clones with same target sequence:
(none)
