Construct: sgRNA BRDN0001146944

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCTAAAGTCAGACTACTCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TEX14 (56155)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76831

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000017.11 17 58586015 + TEX14 NNNAAAGTCAGACTACTCTG NGG 0 1.0 Tier I
2 NC_000009.12 9 37537670 + FBXO10 NNNAAAGTCAGAGTCCTCTG NGG 2 0.0372 Tier I
3 NC_000007.14 7 102459750 + ALKBH4 NNNAAAGTCAGACTCCTCTG NGC 2 0.0061 Tier I
4 NC_000008.11 8 67199175 + ARFGEF1 NNNAGAGTCAAACTACTCTG NGG 2 0.72 Tier II
5 NC_000001.11 1 111602520 + RAP1A NNNAGAGTTAGACTACTCTG NGG 2 0.63 Tier II
6 NC_000001.11 1 13993811 - KAZN NNNGAAGTCAAACTACTCTG NGG 2 0.625 Tier II
7 NC_000001.11 1 239464806 + CHRM3 NNNAAAGTCTGACTACCCTG NGG 2 0.6228 Tier II
8 NC_000007.14 7 86725398 - GRM3 NNNAAAGTCTGACTACCCTG NGG 2 0.6228 Tier II
9 NC_000010.11 10 88201463 + RNLS NNNAAAGTCTGACTACCCTG NGG 2 0.6228 Tier II
10 NC_000017.11 17 17097826 - MPRIP NNNAAAGTCAGACAAATCTG NGG 2 0.619 Tier II
11 NC_000011.10 11 10145305 - SBF2 NNNGAAGTCTGACTACTCTG NGG 2 0.5515 Tier II
12 NC_000003.12 3 130463548 + COL6A5 NNNAAAGTCAGGCTACTCTC NGG 2 0.3095 Tier II
13 NC_000013.11 13 25912735 - ATP8A2 NNNAAAGTAAGACTACTCTG NAG 2 0.2222 Tier II
14 NC_000023.11 X 11210385 - ARHGAP6 NNNAAAGTCAGACTCCTCTT NGG 2 0.1909 Tier II
15 NC_000010.11 10 92208312 + CPEB3 NNNAAAGTCAGACTCCTTTG NGG 2 0.1753 Tier II
16 NC_000010.11 10 71485910 - CDH23 NNNTAAGTCAGACTTCTCTG NGG 2 0.1273 Tier II
17 NC_000012.12 12 9052350 - KLRG1 NNNAAAGTCAGACTATGCTG NGG 2 0.0362 Tier II
18 NC_000020.11 20 14838404 + MACROD2 NNNAAAGTCAGACTAATCTG NGT 2 0.0161 Tier II
19 NC_000003.12 3 152353118 + MBNL1 NNNAAAGTCAGAGTACTCTG NGA 2 0.0095 Tier II
20 NC_000005.10 5 122455507 - SNCAIP NNNAAAGTCATACTACTCTG NGC 2 0.0085 Tier II
21 NC_000011.10 11 132334683 - NTM NNNAAAGTCAGACTACTGTG NTG 2 0.0052 Tier II
22 NC_000001.11 1 111602520 + LINC01160 NNNAGAGTTAGACTACTCTG NGG 2 0.63 Tier III
23 NC_000010.11 10 88201463 + LOC101929727 NNNAAAGTCTGACTACCCTG NGG 2 0.6228 Tier III
24 NC_000007.14 7 140927574 - LOC105375536 NNNGAAGTCTGACTACTCTG NGG 2 0.5515 Tier III
25 NC_000022.11 22 34212125 - LINC01643 NNNGAAGTCAGACTGCTCTG NGG 2 0.4063 Tier III
26 NC_000020.11 20 24315429 - LOC105372577 NNNCAAGTGAGACTACTCTG NGG 2 0.2185 Tier III
27 NC_000005.10 5 122455507 - MGC32805 NNNAAAGTCATACTACTCTG NGC 2 0.0085 Tier III
28 NC_000005.10 5 122455507 - LOC107986446 NNNAAAGTCATACTACTCTG NGC 2 0.0085 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 29229797 + Cfap36 NNNAAAATCTGACTACTCTG NGG 2 0.8824 Tier II
2 NC_000073.6 7 110823603 + Rnf141 NNNAGGGTCAGACTACTCTG NGG 2 0.5143 Tier II
3 NC_000072.6 6 129658344 - Klrc2 NNNAGAGTCAGATTACTCTG NGG 2 0.504 Tier II
4 NC_000072.6 6 129676831 - Klrc1 NNNAGAGTCAGATTACTCTG NGG 2 0.504 Tier II
5 NC_000068.7 2 25235612 + Rnf224 NNNAGAGTCAGACTACTTTG NGG 2 0.4629 Tier II
6 NC_000081.6 15 21123468 - Cdh12 NNNAAACTCAGACTGCTCTG NGG 2 0.4469 Tier II
7 NC_000079.6 13 117807093 + Hcn1 NNNAAAGACAGACTACACTG NGG 2 0.4267 Tier II
8 NC_000082.6 16 40849443 - Lsamp NNNAAAGTCAAAATACTCTG NGG 2 0.3846 Tier II
9 NC_000068.7 2 54656310 - Galnt13 NNNAAAGTGAGACAACTCTG NGG 2 0.3832 Tier II
10 NC_000081.6 15 35861170 + Vps13b NNNAAAGTCACATTACTCTG NGG 2 0.3 Tier II
11 NC_000080.6 14 11143462 + Fhit NNNAAAGTCAGACAACTCTG NAG 2 0.1605 Tier II
12 NC_000071.6 5 36248515 + Sorcs2 NNNAAAGTCACACTACTCAG NGG 2 0.1224 Tier II
13 NC_000072.6 6 71615647 - Kdm3a NNNGAAGTCAGACTAGTCTG NGG 2 0.0962 Tier II
14 NC_000069.6 3 33120456 - Pex5l NNNAAAGTCAGCCTTCTCTG NGG 2 0.0526 Tier II
15 NC_000070.6 4 6815656 - Tox NNNAAAGTCAGAGTTCTCTG NGG 2 0.0273 Tier II
16 NC_000079.6 13 94142554 - Lhfpl2 NNNAAAATCAGACTACTCTG NGT 2 0.0161 Tier II
17 NC_000077.6 11 94697671 + Gm11541 NNNGAAGTCAGACTACTCTG NGT 2 0.0101 Tier II
18 NC_000075.6 9 7950970 - Yap1 NNNAAAGTCAGACTCCTCTG NGC 2 0.0061 Tier II
19 NC_000068.7 2 48346206 - Gm13481 NNNAAAGACAGACTACTCTT NGG 2 0.56 Tier III
20 NC_000067.6 1 190195116 + Prox1os NNNTAAGTCAGGCTACTCTG NGG 2 0.4596 Tier III
21 NC_000067.6 1 103340062 - Gm18728 NNNAAAGTCAGACTACTCAA NGG 2 0.2679 Tier III
22 NC_000069.6 3 7683601 + Gm16685 NNNAAAGTCAGAATACTTTG NGG 2 0.2473 Tier III
23 NC_000068.7 2 30670551 - Gm14486 NNNAGAGTCAGACCACTCTG NGG 2 0.2057 Tier III
Download CSV

Other clones with same target sequence:

(none)