Construct: sgRNA BRDN0001146945
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGGTAACACTCTCCCGTGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKD3 (23683)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75953
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
37316319 |
+ |
PRKD3 |
NNNTAACACTCTCCCGTGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
7215813 |
+ |
C1GALT1 |
NNNTAACATCCTCCCGTGTG |
NGG |
2 |
0.4667 |
Tier II |
| 3 |
NC_000015.10 |
15 |
101485113 |
+ |
PCSK6 |
NNNTAACACTCTTCCGTGTC |
NGG |
2 |
0.3 |
Tier II |
| 4 |
NC_000015.10 |
15 |
101485113 |
+ |
LOC124903566 |
NNNTAACACTCTTCCGTGTC |
NGG |
2 |
0.3 |
Tier II |
| 5 |
NC_000010.11 |
10 |
5916125 |
+ |
FBH1 |
NNNTAACACTCGCCTGTGTG |
NGG |
2 |
0.0333 |
Tier II |
| 6 |
NC_000012.12 |
12 |
38710649 |
+ |
CPNE8 |
NNNTAACACTCTCCTCTGTG |
NGG |
2 |
0.0 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
79012706 |
+ |
Prkd3 |
NNNTAACACTCTCTCGTGTG |
NGG |
1 |
0.7333 |
Tier I |
| 2 |
NC_000079.6 |
13 |
15713685 |
- |
Gli3 |
NNNTCACACTCGCCCGTGTG |
NGG |
2 |
0.25 |
Tier II |
| 3 |
NC_000068.7 |
2 |
7195563 |
- |
Celf2 |
NNNAAACACTCTCCAGTGTG |
NGG |
2 |
0.1058 |
Tier II |
| 4 |
NC_000070.6 |
4 |
49114453 |
- |
Plppr1 |
NNNTAACACACTCCTGTGTG |
NGG |
2 |
0.0571 |
Tier II |
| 5 |
NC_000074.6 |
8 |
104541461 |
- |
Car7 |
NNNAAACACTCTCCCGGGTG |
NGG |
2 |
0.056 |
Tier II |
| 6 |
NC_000067.6 |
1 |
64555195 |
- |
Creb1 |
NNNTAACATTCTCCCCTGTG |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000079.6 |
13 |
7387615 |
- |
Gm6437 |
NNNTAACACTCTCCCATCTG |
NGG |
2 |
0.4762 |
Tier III |
Other clones with same target sequence:
(none)
