Construct: sgRNA BRDN0001146948
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CAGATATGTGTAGAACGATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PHKA2 (5256)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77844
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
18920164 |
+ |
PHKA2 |
NNNATATGTGTAGAACGATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
34538427 |
+ |
PARD3 |
NNNATAGGTGTAGAAAGATG |
NGG |
2 |
0.5882 |
Tier II |
| 3 |
NC_000008.11 |
8 |
109403708 |
- |
PKHD1L1 |
NNNATATGTGTAGAATGATA |
NGG |
2 |
0.2885 |
Tier II |
| 4 |
NC_000005.10 |
5 |
128125881 |
- |
SLC12A2 |
NNNATATGTGTACAAGGATG |
NGG |
2 |
0.0648 |
Tier II |
| 5 |
NC_000010.11 |
10 |
1759701 |
- |
LOC105376345 |
NNNCTATGTGTAGAACTATG |
NGG |
2 |
0.0882 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
5043411 |
- |
Rgs20 |
NNNATATATGTAGAACAATG |
NGG |
2 |
0.9333 |
Tier II |
| 2 |
NC_000082.6 |
16 |
35035825 |
+ |
Hacd2 |
NNNATATGTTTAGAACAATG |
NGG |
2 |
0.7583 |
Tier II |
| 3 |
NC_000080.6 |
14 |
34572491 |
- |
Ldb3 |
NNNATGTGTGTAGAAAGATG |
NGG |
2 |
0.7143 |
Tier II |
| 4 |
NC_000084.6 |
18 |
67496744 |
+ |
Spire1 |
NNNATATTTGTAGAAAGATG |
NGG |
2 |
0.625 |
Tier II |
| 5 |
NC_000082.6 |
16 |
72869783 |
+ |
Robo1 |
NNNATATGTTTAGAATGATG |
NGG |
2 |
0.25 |
Tier II |
| 6 |
NC_000075.6 |
9 |
56415228 |
- |
Peak1 |
NNNATATGTGTAGAATGATG |
NAG |
2 |
0.0798 |
Tier II |
| 7 |
NC_000076.6 |
10 |
112411852 |
+ |
Kcnc2 |
NNNATATTTGTAGAACGATG |
NTG |
2 |
0.0244 |
Tier II |
| 8 |
NC_000068.7 |
2 |
167791698 |
- |
Gm32513 |
NNNACATGTGTAGAAGGATG |
NGG |
2 |
0.1538 |
Tier III |
| 9 |
NC_000074.6 |
8 |
91823377 |
- |
Irx3os |
NNNACATGTGTAGAACGATG |
NCG |
2 |
0.1071 |
Tier III |
| 10 |
NC_000074.6 |
8 |
91823377 |
- |
Gm39223 |
NNNACATGTGTAGAACGATG |
NCG |
2 |
0.1071 |
Tier III |
| 11 |
NC_000068.7 |
2 |
151405024 |
+ |
LOC100125594 |
NNNATATGTGCAGAAGGATG |
NGG |
2 |
0.1026 |
Tier III |
Other clones with same target sequence:
(none)
