Construct: sgRNA BRDN0001146949
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AACTTCCTGATTGGTCGACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CSNK1G1 (53944)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77440
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
64214048 |
- |
CSNK1G1 |
NNNTTCCTGATTGGTCGACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
20768704 |
- |
FOCAD |
NNNTTCATGATTGGTCAACA |
NGG |
2 |
0.7 |
Tier II |
| 3 |
NC_000011.10 |
11 |
62831809 |
+ |
STX5 |
NNNTTCCTGATTGGACGGCA |
NGG |
2 |
0.2316 |
Tier II |
| 4 |
NC_000001.11 |
1 |
64490123 |
- |
CACHD1 |
NNNTTCCTGATCGGTGGACA |
NGG |
2 |
0.1457 |
Tier II |
| 5 |
NC_000005.10 |
5 |
131574792 |
- |
RAPGEF6 |
NNNTGCCTGATTGGTCGCCA |
NGG |
2 |
0.1219 |
Tier II |
| 6 |
NC_000003.12 |
3 |
190532579 |
- |
IL1RAP |
NNNTTCCTTATTGGTGGACA |
NGG |
2 |
0.0821 |
Tier II |
| 7 |
NC_000013.11 |
13 |
35559226 |
+ |
NBEA |
NNNTTCCTGATTGGTGGACA |
NTG |
2 |
0.006 |
Tier II |
| 8 |
NC_000004.12 |
4 |
88452505 |
- |
LOC102723458 |
NNNTTCCTGATTGGTTGACA |
NAG |
2 |
0.0798 |
Tier III |
| 9 |
NC_000007.14 |
7 |
122932535 |
- |
LOC105375482 |
NNNTTCCTGCTTGGTGGACA |
NGG |
2 |
0.0513 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
66002124 |
+ |
Csnk1g1 |
NNNTTCTTGATTGGCCGACA |
NGG |
2 |
0.2216 |
Tier I |
| 2 |
NC_000079.6 |
13 |
74031295 |
- |
Tppp |
NNNTTCCTGATTGGTCGAAA |
NGC |
2 |
0.0095 |
Tier I |
| 3 |
NC_000069.6 |
3 |
55114092 |
- |
Spg20 |
NNNTTCCTGTTTGGTAGACA |
NGG |
2 |
0.8824 |
Tier II |
| 4 |
NC_000079.6 |
13 |
49111396 |
- |
Wnk2 |
NNNTTCCTGATTGATTGACA |
NGG |
2 |
0.2308 |
Tier II |
| 5 |
NC_000068.7 |
2 |
126854383 |
+ |
Trpm7 |
NNNTTCCTGATTGGACCACA |
NGG |
2 |
0.1362 |
Tier II |
| 6 |
NC_000076.6 |
10 |
127857523 |
+ |
Rdh19 |
NNNTTCCTGAGTGGTTGACA |
NGG |
2 |
0.1231 |
Tier II |
| 7 |
NC_000067.6 |
1 |
37804312 |
- |
Tsga10 |
NNNTTCCTGATTGTTTGACA |
NGG |
2 |
0.0821 |
Tier II |
| 8 |
NC_000076.6 |
10 |
95731719 |
+ |
Gm47708 |
NNNTTCCTGATTGGTCAACA |
NAG |
2 |
0.242 |
Tier III |
Other clones with same target sequence:
(none)
