Construct: sgRNA BRDN0001146954
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCTTCGATGATATTGGACAC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- AK5 (26289)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76555
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
77417673 |
+ |
AK5 |
NNNTCGATGATATTGGACAC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
2498738 |
- |
PLCH2 |
NNNTCGATGATCTGGGACAC |
NGG |
2 |
0.0 |
Tier I |
| 3 |
NC_000017.11 |
17 |
4302542 |
+ |
UBE2G1 |
NNNTGGATGATACTGGACAC |
NGG |
2 |
0.4737 |
Tier II |
| 4 |
NC_000013.11 |
13 |
93355965 |
+ |
GPC6 |
NNNTCTGTGATATTGGACAC |
NGG |
2 |
0.4706 |
Tier II |
| 5 |
NC_000009.12 |
9 |
9631723 |
+ |
PTPRD |
NNNTTGATGATATTGGACAC |
NGC |
2 |
0.0141 |
Tier II |
| 6 |
NC_000013.11 |
13 |
79547615 |
- |
NDFIP2 |
NNNTTGATGATATTGGACAC |
NGT |
2 |
0.0103 |
Tier II |
| 7 |
NC_000007.14 |
7 |
3672073 |
+ |
SDK1 |
NNNTCCATGATATGGGACAC |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000017.11 |
17 |
4302542 |
+ |
MFSD1P1 |
NNNTGGATGATACTGGACAC |
NGG |
2 |
0.4737 |
Tier III |
| 9 |
NC_000001.11 |
1 |
165510742 |
- |
LRRC52-AS1 |
NNNTGGGTGATATTGGACAC |
NGG |
2 |
0.4235 |
Tier III |
| 10 |
NC_000004.12 |
4 |
12941485 |
+ |
LOC105374493 |
NNNTGGATGATATTGGACAT |
NGG |
2 |
0.18 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000079.6 |
13 |
62837251 |
+ |
Fbp2 |
NNNTCGATGATATAGGCCAC |
NGG |
2 |
0.1092 |
Tier I |
| 2 |
NC_000073.6 |
7 |
115743271 |
- |
Sox6 |
NNNTCTAGGATATTGGACAC |
NGG |
2 |
0.4889 |
Tier II |
| 3 |
NC_000085.6 |
19 |
39404038 |
+ |
Cyp2c38 |
NNNTAGTTGATATTGGACAC |
NGG |
2 |
0.25 |
Tier II |
| 4 |
NC_000085.6 |
19 |
39404038 |
+ |
Cyp2c39 |
NNNTAGTTGATATTGGACAC |
NGG |
2 |
0.25 |
Tier II |
Other clones with same target sequence:
(none)
