Construct: sgRNA BRDN0001146956

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTGCCCTTGCCCGAGCCGGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AK4 (205)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77695

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 65148450 - AK4 NNNCCCTTGCCCGAGCCGGG NGG 0 1.0 Tier I
2 NC_000001.11 1 41361921 + FOXO6 NNNTCCTCGCCCGAGCCGGG NGG 2 0.8 Tier I
3 NC_000012.12 12 53973752 - HOXC11 NNNCCCTTGCCGGAGCAGGG NGG 2 0.2074 Tier I
4 NC_000001.11 1 11926248 - KIAA2013 NNNCCCTTGCCCGCGCCTGG NGG 2 0.1429 Tier I
5 NC_000009.12 9 4741040 + AK3 NNNCCCTTGCCCGAGCCCGG NGC 2 0.0106 Tier I
6 NC_000017.11 17 31345561 - NF1 NNNCCCTTGCCCGAGCCGGG NGG 0 1.0 Tier II
7 NC_000013.11 13 19864048 + ZMYM2 NNNCCCTCGCCCGAGCCCGG NGG 2 0.4762 Tier II
8 NC_000014.9 14 63853464 - SYNE2 NNNCGCCTGCCCGAGCCGGG NGG 2 0.4125 Tier II
9 NC_000011.10 11 133082312 - OPCML NNNCCTTTGCCCCAGCCGGG NGG 2 0.391 Tier II
10 NC_000020.11 20 57710121 + PMEPA1 NNNCCCCTCCCCGAGCCGGG NGG 2 0.3702 Tier II
11 NC_000009.12 9 133955123 - VAV2 NNNCCCTTGGCCGAGCCTGG NGG 2 0.2593 Tier II
12 NC_000022.11 22 29306935 - GAS2L1 NNNCCCTTCCCCGAGCCGGC NGG 2 0.2308 Tier II
13 NC_000020.11 20 15134246 + MACROD2 NNNACCTTGCCCGAGCCGGG NAG 2 0.2183 Tier II
14 NC_000015.10 15 26867006 - GABRA5 NNNCCCTTGGCCGAGCTGGG NGG 2 0.1815 Tier II
15 NC_000016.10 16 14990171 - PDXDC1 NNNCCCTTGCCCGCGCCTGG NGG 2 0.1429 Tier II
16 NC_000022.11 22 46071199 + LOC124905135 NNNCCCTTGCCCGGGGCGGG NGG 2 0.0718 Tier II
17 NC_000012.12 12 128945159 + GLT1D1 NNNCCCTTGCCCGAGCCGGG NGC 1 0.0222 Tier II
18 NC_000006.12 6 34392452 - NUDT3 NNNCCCTTGGCCGAGCCGGG NGC 2 0.0086 Tier II
19 NC_000006.12 6 34392452 - RPS10-NUDT3 NNNCCCTTGGCCGAGCCGGG NGC 2 0.0086 Tier II
20 NC_000012.12 12 31616398 + AK4P3 NNNCCCTTGCCCGAGCCGGG NGG 0 1.0 Tier III
21 NC_000017.11 17 31345561 - AK4P1 NNNCCCTTGCCCGAGCCGGG NGG 0 1.0 Tier III
22 NC_000010.11 10 131785298 + LINC01164 NNNCCCTGGCCCGAGCCGAG NGG 2 0.5238 Tier III
23 NC_000005.10 5 55552513 + AK4P2 NNNCCCTTGCCCGAGCAGGG NGG 1 0.4667 Tier III
24 NC_000012.12 12 53973752 - HOTAIR NNNCCCTTGCCGGAGCAGGG NGG 2 0.2074 Tier III
25 NC_000016.10 16 14990171 - LOC728138 NNNCCCTTGCCCGCGCCTGG NGG 2 0.1429 Tier III
26 NC_000012.12 12 128945159 + AK3P6 NNNCCCTTGCCCGAGCCGGG NGC 1 0.0222 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000070.6 4 101419863 - Ak4 NNNCCCTTGCCAGAGCCGGG NGG 1 0.7143 Tier I
2 NC_000070.6 4 120287263 - Foxo6 NNNGCCTCGCCCGAGCCGGG NGG 2 0.5 Tier I
3 NC_000081.6 15 102955036 - Hoxc11 NNNCCCTTGCCGGAGCAGGG NGG 2 0.2074 Tier I
4 NC_000075.6 9 119542970 - Scn5a NNNCCTTTGCCAGAGCCGGG NGG 2 0.6633 Tier II
5 NC_000085.6 19 6850213 - Ccdc88b NNNCCCTTGCCCCAGCCTGG NGG 2 0.2807 Tier II
6 NC_000085.6 19 57046497 + Ablim1 NNNGCCTTGCCTGAGCCGGG NGG 2 0.2692 Tier II
7 NC_000067.6 1 75479669 + Tmem198 NNNCCCTTCCCCGAGCCGGG NGC 2 0.012 Tier II
8 NC_000068.7 2 72633978 - Ak3l2-ps NNNCCCTTGCCAGAGCCGGG NGG 1 0.7143 Tier III
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Other clones with same target sequence:

(none)