Construct: sgRNA BRDN0001146958
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGTGCTCGAACATAAAACTT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GALK2 (2585)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76768
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
49201214 |
- |
GALK2 |
NNNGCTCGAACATAAAACTT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000014.9 |
14 |
92451631 |
- |
SLC24A4 |
NNNGCTGGAAGATAAAACTT |
NGG |
2 |
0.1176 |
Tier II |
| 3 |
NC_000008.11 |
8 |
37865859 |
+ |
RAB11FIP1 |
NNNGCACGAACATAAATCTT |
NGG |
2 |
0.1156 |
Tier II |
| 4 |
NC_000004.12 |
4 |
184696447 |
- |
CENPU |
NNNGCTTGAACATAATACTT |
NGG |
2 |
0.0 |
Tier II |
| 5 |
NC_000001.11 |
1 |
99107272 |
+ |
PLPPR5-AS1 |
NNNGCTGGAAGATAAAACTT |
NGG |
2 |
0.1176 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
125878330 |
- |
Galk2 |
NNNGCTCGAACATAAAACTT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000071.6 |
5 |
3475414 |
- |
Cdk6 |
NNNGCTTGAAAATAAAACTT |
NGG |
2 |
0.6094 |
Tier II |
| 3 |
NC_000075.6 |
9 |
69347349 |
+ |
Rora |
NNNGCTAGGACATAAAACTT |
NGG |
2 |
0.5 |
Tier II |
| 4 |
NC_000068.7 |
2 |
140686102 |
- |
Macrod2 |
NNNGTTCTAACATAAAACTT |
NGG |
2 |
0.3977 |
Tier II |
| 5 |
NC_000078.6 |
12 |
37504525 |
- |
Agmo |
NNNGCACGAACATAAAACAT |
NGG |
2 |
0.2476 |
Tier II |
| 6 |
NC_000079.6 |
13 |
100100158 |
+ |
Bdp1 |
NNNGCTCTACCATAAAACTT |
NGG |
2 |
0.2083 |
Tier II |
Other clones with same target sequence:
(none)
