Construct: sgRNA BRDN0001146965
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAACCAGGCGAATGTGACCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TNNI3K (51086)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77897
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
74354005 |
- |
TNNI3K |
NNNCCAGGCGAATGTGACCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
74354005 |
- |
FPGT-TNNI3K |
NNNCCAGGCGAATGTGACCG |
NGG |
0 |
1.0 |
Tier I |
| 3 |
NC_000017.11 |
17 |
6095189 |
+ |
WSCD1 |
NNNACAGGCCAATGTGACCG |
NGG |
2 |
0.3368 |
Tier I |
| 4 |
NC_000004.12 |
4 |
121037397 |
+ |
NDNF |
NNNCCAGGCCAAAGTGACCG |
NGG |
2 |
0.2769 |
Tier I |
| 5 |
NC_000016.10 |
16 |
2108419 |
- |
PKD1 |
NNNCCAGGCCAATGTGACGG |
NGG |
2 |
0.05 |
Tier I |
| 6 |
NC_000010.11 |
10 |
77077893 |
+ |
KCNMA1 |
NNNACAGGCGAATGTGACCT |
NGG |
2 |
0.5895 |
Tier II |
| 7 |
NC_000007.14 |
7 |
630242 |
+ |
PRKAR1B |
NNNCCAGGCGAAGGTGACCG |
NGG |
1 |
0.2609 |
Tier II |
| 8 |
NC_000010.11 |
10 |
84139582 |
+ |
GHITM |
NNNCGAGGCGAAGGTGACCG |
NGG |
2 |
0.1565 |
Tier II |
| 9 |
NC_000016.10 |
16 |
15135816 |
- |
PDXDC1 |
NNNCCAGGCCAATGTGACGG |
NGG |
2 |
0.05 |
Tier II |
| 10 |
NC_000016.10 |
16 |
15135816 |
- |
PKD1P6 |
NNNCCAGGCCAATGTGACGG |
NGG |
2 |
0.05 |
Tier III |
| 11 |
NC_000016.10 |
16 |
16367202 |
+ |
PKD1P2 |
NNNCCAGGCCAATGTGACGG |
NGG |
2 |
0.05 |
Tier III |
| 12 |
NC_000016.10 |
16 |
18384980 |
- |
PKD1P5 |
NNNCCAGGCCAATGTGACGG |
NGG |
2 |
0.05 |
Tier III |
| 13 |
NC_000016.10 |
16 |
18384980 |
- |
PKD1P5-LOC105376752 |
NNNCCAGGCCAATGTGACGG |
NGG |
2 |
0.05 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
24576077 |
+ |
Pkd1 |
NNNCCAGGCCAATGTGACAG |
NGG |
2 |
0.1714 |
Tier I |
| 2 |
NC_000077.6 |
11 |
6598067 |
- |
Nacad |
NNNCCAGGCCAATGTGACCC |
NGG |
2 |
0.1714 |
Tier I |
| 3 |
NC_000067.6 |
1 |
59580686 |
+ |
Gm973 |
NNNCCAGGCGAGTGTGAGCG |
NGG |
2 |
0.0963 |
Tier II |
| 4 |
NC_000071.6 |
5 |
67282009 |
+ |
Tmem33 |
NNNCCAGGCGAAGGTCACCG |
NGG |
2 |
0.0 |
Tier II |
| 5 |
NC_000078.6 |
12 |
32112962 |
+ |
5430401H09Rik |
NNNCCAGGCGAGTGTGACCC |
NGG |
2 |
0.3095 |
Tier III |
| 6 |
NC_000078.6 |
12 |
32112962 |
+ |
Gm51991 |
NNNCCAGGCGAGTGTGACCC |
NGG |
2 |
0.3095 |
Tier III |
Other clones with same target sequence:
(none)
