Construct: sgRNA BRDN0001146966
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTACCACCCATTACCTATTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EIF2AK3 (9451)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77168
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
88579521 |
+ |
EIF2AK3 |
NNNCCACCCATTACCTATTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000023.11 |
X |
49324830 |
- |
GAGE12J |
NNNACACCCATTACCTATTG |
NGG |
1 |
0.8421 |
Tier II |
| 3 |
NC_000023.11 |
X |
49334376 |
- |
GAGE13 |
NNNACACCCATTACCTATTG |
NGG |
1 |
0.8421 |
Tier II |
| 4 |
NC_000023.11 |
X |
49534954 |
- |
GAGE12C |
NNNACACCCATTACCTATTG |
NGG |
1 |
0.8421 |
Tier II |
| 5 |
NC_000023.11 |
X |
49544510 |
- |
GAGE12D |
NNNACACCCATTACCTATTG |
NGG |
1 |
0.8421 |
Tier II |
| 6 |
NC_000023.11 |
X |
49554066 |
- |
GAGE12E |
NNNACACCCATTACCTATTG |
NGG |
1 |
0.8421 |
Tier II |
| 7 |
NC_000023.11 |
X |
49563625 |
- |
GAGE12F |
NNNACACCCATTACCTATTG |
NGG |
1 |
0.8421 |
Tier II |
| 8 |
NC_000023.11 |
X |
49573177 |
- |
GAGE12G |
NNNACACCCATTACCTATTG |
NGG |
1 |
0.8421 |
Tier II |
| 9 |
NC_000023.11 |
X |
49582726 |
- |
GAGE12H |
NNNACACCCATTACCTATTG |
NGG |
1 |
0.8421 |
Tier II |
| 10 |
NC_000023.11 |
X |
49592248 |
- |
GAGE2A |
NNNACACCCATTACCTATTG |
NGG |
1 |
0.8421 |
Tier II |
| 11 |
NC_000023.11 |
X |
49601803 |
- |
GAGE1 |
NNNACACCCATTACCTATTG |
NGG |
1 |
0.8421 |
Tier II |
| 12 |
NC_000023.11 |
X |
55261911 |
+ |
PAGE3 |
NNNACACCCATAACCTATTG |
NGG |
2 |
0.6737 |
Tier II |
| 13 |
NC_000017.11 |
17 |
80211978 |
- |
SGSH |
NNNACACCCATTACCTAATG |
NGG |
2 |
0.5614 |
Tier II |
| 14 |
NC_000001.11 |
1 |
210960665 |
+ |
KCNH1 |
NNNTTACCCATTACCTATTG |
NGG |
2 |
0.5091 |
Tier II |
| 15 |
NC_000001.11 |
1 |
244619017 |
- |
CATSPERE |
NNNCACCCCATTACCTATTG |
NGG |
2 |
0.2597 |
Tier II |
| 16 |
NC_000023.11 |
X |
49343926 |
- |
GAGE12B |
NNNACACCCATTACCTATCG |
NGG |
2 |
0.2323 |
Tier II |
| 17 |
NC_000007.14 |
7 |
154492559 |
+ |
DPP6 |
NNNCCACCCATTCCCTATTC |
NGG |
2 |
0.0902 |
Tier II |
| 18 |
NC_000010.11 |
10 |
127297898 |
+ |
DOCK1 |
NNNCCACCCATTACCTATTG |
NGA |
1 |
0.0694 |
Tier II |
| 19 |
NC_000023.11 |
X |
49306483 |
- |
GAGE10 |
NNNACACCCATTACTTATTG |
NGG |
2 |
0.0561 |
Tier II |
| 20 |
NC_000016.10 |
16 |
17162722 |
+ |
XYLT1 |
NNNCCACCCAATACCTATTG |
NGC |
2 |
0.0167 |
Tier II |
| 21 |
NC_000008.11 |
8 |
74201065 |
- |
LOC105375903 |
NNNCCAACCATTATCTATTG |
NGG |
2 |
0.55 |
Tier III |
| 22 |
NC_000008.11 |
8 |
10479464 |
- |
LINC03022 |
NNNCCACCCATTCCCTATTG |
NGA |
2 |
0.0146 |
Tier III |
| 23 |
NC_000008.11 |
8 |
23759886 |
- |
LOC107986930 |
NNNCCACCCATTCCTTATTG |
NGG |
2 |
0.014 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
125883127 |
+ |
Lypd1 |
NNNCCACCCATTACCCATTG |
NGG |
1 |
0.6667 |
Tier II |
| 2 |
NC_000080.6 |
14 |
10632574 |
- |
Fhit |
NNNCCACACATTACCTCTTG |
NGG |
2 |
0.1147 |
Tier II |
| 3 |
NC_000084.6 |
18 |
76045049 |
- |
Zbtb7c |
NNNCCACCCATTACATATAG |
NGG |
2 |
0.0635 |
Tier II |
Other clones with same target sequence:
(none)
