Construct: sgRNA BRDN0001146967
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGGAAAAGGCGTGATACACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- RIPK1 (8737)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76534
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
3081064 |
+ |
RIPK1 |
NNNAAAAGGCGTGATACACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000020.11 |
20 |
6778855 |
- |
BMP2 |
NNNAAAAGGCGTGATACCCC |
NGG |
2 |
0.0433 |
Tier I |
| 3 |
NC_000015.10 |
15 |
29767345 |
- |
TJP1 |
NNNACAAGGAGTGATACACA |
NGG |
2 |
0.4333 |
Tier II |
| 4 |
NC_000023.11 |
X |
32879106 |
- |
DMD |
NNNAAAAGGCTTGATACACA |
NGG |
1 |
0.3846 |
Tier II |
| 5 |
NC_000007.14 |
7 |
28634582 |
- |
CREB5 |
NNNATAAGGCATGATACACA |
NGG |
2 |
0.3636 |
Tier II |
| 6 |
NC_000009.12 |
9 |
117326584 |
+ |
ASTN2 |
NNNAAAAGGCTTAATACACA |
NGG |
2 |
0.355 |
Tier II |
| 7 |
NC_000008.11 |
8 |
120631122 |
- |
SNTB1 |
NNNAAAAGGCCTGATACTCA |
NGG |
2 |
0.2143 |
Tier II |
| 8 |
NC_000007.14 |
7 |
142166355 |
- |
MGAM2 |
NNNAAAAGGCGTGTTGCACA |
NGG |
2 |
0.1026 |
Tier II |
| 9 |
NC_000001.11 |
1 |
65442046 |
+ |
LEPR |
NNNAAGAGGCGTGAGACACA |
NGG |
2 |
0.0357 |
Tier II |
| 10 |
NC_000001.11 |
1 |
72771754 |
- |
LINC02796 |
NNNAAAAGGCGGGATACACA |
NTG |
2 |
0.0195 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000079.6 |
13 |
34013335 |
+ |
Ripk1 |
NNNCAAAGGTGTGATACACA |
NGG |
2 |
0.3322 |
Tier I |
| 2 |
NC_000080.6 |
14 |
7894730 |
- |
Flnb |
NNNAAAAGGAGAGATACACA |
NGG |
2 |
0.6933 |
Tier II |
| 3 |
NC_000081.6 |
15 |
53240455 |
- |
Ext1 |
NNNAAAATGCTTGATACACA |
NGG |
2 |
0.2404 |
Tier II |
| 4 |
NC_000080.6 |
14 |
105110001 |
- |
Rbm26 |
NNNAAAAGGCATGATACAGA |
NGG |
2 |
0.125 |
Tier II |
| 5 |
NC_000070.6 |
4 |
145262368 |
+ |
Gm36233 |
NNNAATAGGCTTGATACACA |
NGG |
2 |
0.2747 |
Tier III |
Other clones with same target sequence:
(none)
