Construct: sgRNA BRDN0001146970
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CAAAAGGCCGGATATTTACC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- AAK1 (22848)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77935
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
69527278 |
- |
AAK1 |
NNNAAGGCCGGATATTTACC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000013.11 |
13 |
113653411 |
- |
ATP4B |
NNNAAGGCCGGATGTTTACG |
NGG |
2 |
0.0275 |
Tier I |
| 3 |
NC_000013.11 |
13 |
113653411 |
- |
GRK1 |
NNNAAGGCCGGATGTTTACG |
NGG |
2 |
0.0275 |
Tier II |
| 4 |
NC_000009.12 |
9 |
109704201 |
+ |
PALM2AKAP2 |
NNNCAGGCCGGATATTTACC |
NGC |
2 |
0.0078 |
Tier II |
| 5 |
NC_000002.12 |
2 |
131757220 |
+ |
LINC03124 |
NNNAAGGCCTGATATCTACC |
NGG |
2 |
0.5417 |
Tier III |
| 6 |
NC_000020.11 |
20 |
25773370 |
- |
FAM182B |
NNNAAGGCCTGATGTTTACC |
NGG |
2 |
0.3792 |
Tier III |
| 7 |
NC_000020.11 |
20 |
26083144 |
+ |
FAM182A |
NNNAAGGCCTGATGTTTACC |
NGG |
2 |
0.3792 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
86949584 |
+ |
Aak1 |
NNNAAGGCCGGATATTTACC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000077.6 |
11 |
62031869 |
+ |
Specc1 |
NNNAAGGCCGGATATCTATC |
NGG |
2 |
0.3077 |
Tier II |
Other clones with same target sequence:
(none)
