Construct: sgRNA BRDN0001146971

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGGAGAATATCTCTGAGTGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRPS1 (5631)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76201

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000023.11 X 107642444 + PRPS1 NNNAGAATATCTCTGAGTGG NGG 0 1.0 Tier I
2 NC_000007.14 7 18027300 - PRPS1L1 NNNAGAATATCCCTGAGTGG NAG 2 0.2456 Tier I
3 NC_000007.14 7 95530836 + ASB4 NNNAGAATAACTCTGAGTGG NGG 1 0.8571 Tier II
4 NC_000002.12 2 124477044 - CNTNAP5 NNNAAAATATCTCTGAATGG NGG 2 0.8089 Tier II
5 NC_000020.11 20 14949008 - MACROD2 NNNAGAATATCTCAGAATGG NGG 2 0.5778 Tier II
6 NC_000011.10 11 126621227 + KIRREL3 NNNAGAACAGCTCTGAGTGG NGG 2 0.5 Tier II
7 NC_000006.12 6 100567185 - ASCC3 NNNAGATCATCTCTGAGTGG NGG 2 0.4375 Tier II
8 NC_000018.10 18 37350953 - CELF4 NNNAGAAGCTCTCTGAGTGG NGG 2 0.419 Tier II
9 NC_000004.12 4 54665450 + KIT NNNAGATTATCTCTGAGTGA NGG 2 0.4102 Tier II
10 NC_000002.12 2 23986550 - UBXN2A NNNTGATTATCTCTGAGTGG NGG 2 0.2784 Tier II
11 NC_000012.12 12 26576287 - ITPR2 NNNTGATTATCTCTGAGTGG NGG 2 0.2784 Tier II
12 NC_000023.11 X 50461447 + DGKK NNNTGATTATCTCTGAGTGG NGG 2 0.2784 Tier II
13 NC_000023.11 X 136318782 + ADGRG4 NNNTGATTATCTCTGAGTGG NGG 2 0.2784 Tier II
14 NC_000007.14 7 20626928 + ABCB5 NNNAGGATATTTCTGAGTGG NGG 2 0.2198 Tier II
15 NC_000002.12 2 97779092 - TMEM131 NNNAGAATCTCTCTGAGGGG NGG 2 0.1905 Tier II
16 NC_000002.12 2 206025164 - INO80D NNNAGAATATCTCTAGGTGG NGG 2 0.181 Tier II
17 NC_000005.10 5 19965097 + CDH18 NNNAGATTATCTATGAGTGG NGG 2 0.1683 Tier II
18 NC_000014.9 14 29886160 - PRKD1 NNNAGAATGTGTCTGAGTGG NGG 2 0.1667 Tier II
19 NC_000005.10 5 128186362 - SLC12A2 NNNAGAATATTTCTGAGTGC NGG 2 0.1319 Tier II
20 NC_000023.11 X 129794957 + SASH3 NNNAGAGTATCTGTGAGTGG NGG 2 0.0963 Tier II
21 NC_000002.12 2 214181557 - SPAG16 NNNAGAATATCTGTGAGTGG NGC 2 0.003 Tier II
22 NC_000010.11 10 26239716 + GAD2 NNNAAAATATCTCTGTGTGG NGG 2 0.0 Tier II
23 NC_000009.12 9 115199946 + DELEC1 NNNAGAATATCTTTGAGTGG NGG 1 0.7 Tier III
24 NC_000004.12 4 184993876 + LINC02437 NNNAAAATATCTCTGAGAGG NGG 2 0.5778 Tier III
25 NC_000006.12 6 18536409 - MIR548A1HG NNNAGAATATCTCAGAGGGG NGG 2 0.2063 Tier III
26 NC_000016.10 16 52365262 - LOC107984901 NNNAGATTATCTCTGAGTGG NAG 2 0.1134 Tier III
27 NC_000007.14 7 72866944 + SPDYE7P NNNAGAATAGCTCGGAGTGG NGG 2 0.0 Tier III
28 NC_000003.12 3 161150607 + LOC124906300 NNNAGAATATCTTGGAGTGG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000086.7 X 169982032 + Mid1 NNNAGAATATCACTGAGAGG NGG 2 0.5333 Tier I
2 NC_000078.6 12 34985371 + Prps1l1 NNNAGAATATTTCTGACTGG NGG 2 0.0724 Tier I
3 NC_000078.6 12 57238909 + Prps1l3 NNNAGAATATATCTGAGTGG NGA 2 0.0521 Tier I
4 NC_000086.7 X 140468913 + Prps1 NNNAGAATATATCTGAGTGG NGA 2 0.0521 Tier I
5 NC_000081.6 15 95481117 + Nell2 NNNAGAAAATCTCTAAGTGG NGG 2 0.7529 Tier II
6 NC_000068.7 2 105928321 + Immp1l NNNAGAACATCTCTGAGTTG NGG 2 0.6667 Tier II
7 NC_000086.7 X 139222738 - Pwwp3b NNNAGAGTATCTCTGAGTGA NGG 2 0.6618 Tier II
8 NC_000078.6 12 119998341 - Tmem196 NNNAGAATAGCTCTGAATGG NGG 2 0.4667 Tier II
9 NC_000067.6 1 192433169 + Kcnh1 NNNAGAATGTCTCTGAGAGG NGG 2 0.4444 Tier II
10 NC_000083.6 17 24292733 - Abca17 NNNAAATTATCTCTGAGTGG NGG 2 0.3792 Tier II
11 NC_000084.6 18 59356546 + Chsy3 NNNAGATTATCTCTGAGTGT NGG 2 0.3062 Tier II
12 NC_000074.6 8 47409875 + Stox2 NNNAGAATATTTCTAAGTGG NGG 2 0.2896 Tier II
13 NC_000070.6 4 19550026 - Cpne3 NNNAGAATATCTCTGAGTTC NGG 2 0.2857 Tier II
14 NC_000069.6 3 14050648 + Ralyl NNNAGAATATCTCTTAGTGT NGG 2 0.1 Tier II
15 NC_000078.6 12 57238909 + Mipol1 NNNAGAATATATCTGAGTGG NGA 2 0.0521 Tier II
16 NC_000080.6 14 59509359 - Cab39l NNNAGAATATGTCTGAGTGG NCG 2 0.0268 Tier II
17 NC_000080.6 14 79903726 + Gm6999 NNNAGAACATCCCTGAGTGG NGG 2 0.9474 Tier III
18 NC_000087.7 Y 90757448 + Mid1-ps1 NNNAGAATATCACTGAGAGG NGG 2 0.5333 Tier III
19 NC_000087.7 Y 90841804 + LOC108168645 NNNAGAATATCACTGAGAGG NGG 2 0.5333 Tier III
20 NC_000071.6 5 65753041 + Gm46945 NNNAGAGGATCTCTGAGTGG NGG 2 0.5176 Tier III
21 NC_000072.6 6 140821938 + Gm30215 NNNAGAAGATCTCTGAGAGG NGG 2 0.4889 Tier III
22 NC_000079.6 13 48158431 - A330033J07Rik NNNAGAAGATCTCTGAGTGC NGG 2 0.3143 Tier III
23 NC_000067.6 1 134530976 + Gm46159 NNNAGCATTTCTCTGAGTGG NGG 2 0.2727 Tier III
24 NC_000075.6 9 41489064 + Mir100hg NNNAGTATATGTCTGAGTGG NGG 2 0.1786 Tier III
25 NC_000069.6 3 94966942 - B230398E01Rik NNNAGAATATCGCTGAGTGG NAG 2 0.1296 Tier III
26 NC_000078.6 12 34985371 + Gm33864 NNNAGAATATTTCTGACTGG NGG 2 0.0724 Tier III
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Other clones with same target sequence:

(none)