Construct: sgRNA BRDN0001146977
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCACCCAAACATACTAGAGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- IRAK3 (11213)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77715
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
66226746 |
+ |
IRAK3 |
NNNCCCAAACATACTAGAGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
54603823 |
- |
CACNA2D3 |
NNNCACAAAAATACTAGAGT |
NGG |
2 |
0.4952 |
Tier II |
| 3 |
NC_000008.11 |
8 |
132069439 |
- |
HHLA1 |
NNNGCCAAAAATACTAGAGT |
NGG |
2 |
0.4333 |
Tier II |
| 4 |
NC_000011.10 |
11 |
44057871 |
+ |
ACCSL |
NNNCTCAAACATACTAGAGT |
NAG |
2 |
0.165 |
Tier II |
| 5 |
NC_000010.11 |
10 |
32522218 |
- |
CCDC7 |
NNNCCCAAAAATACTAGAGG |
NGG |
2 |
0.1529 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
20785985 |
+ |
Tmc1 |
NNNTCCAAAGATACTAGAGT |
NGG |
2 |
0.3111 |
Tier II |
| 2 |
NC_000068.7 |
2 |
25205040 |
+ |
Nelfb |
NNNCCCAAACATACCAGAAT |
NGG |
2 |
0.1948 |
Tier II |
| 3 |
NC_000068.7 |
2 |
80178326 |
- |
Pde1a |
NNNCCCAAACATTCCAGAGT |
NGG |
2 |
0.0818 |
Tier II |
| 4 |
NC_000068.7 |
2 |
127082809 |
- |
Blvra |
NNNCCCAGACATACGAGAGT |
NGG |
2 |
0.0367 |
Tier II |
| 5 |
NC_000073.6 |
7 |
68254845 |
- |
Pgpep1l |
NNNCCTAAACATAGTAGAGT |
NGG |
2 |
0.0 |
Tier II |
| 6 |
NC_000075.6 |
9 |
104199329 |
+ |
Dnajc13 |
NNNCTCAAACATACTCGAGT |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000075.6 |
9 |
104199329 |
+ |
Gm46130 |
NNNCTCAAACATACTCGAGT |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
