Construct: sgRNA BRDN0001146979

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGCGGATCATATCATCACCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRPS1L1 (221823)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75840

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000007.14 7 18027407 - PRPS1L1 NNNGGATCATATCATCACCA NGG 0 1.0 Tier I
2 NC_000023.11 X 107640972 + PRPS1 NNNAGATCATATTATCACCA NGG 2 0.63 Tier I
3 NC_000023.11 X 12809304 + PRPS2 NNNGGATCACATCATCACCA NGG 1 0.5333 Tier I
4 NC_000009.12 9 125151019 + PPP6C NNNAGATCATATTATCACCA NGG 2 0.63 Tier II
5 NC_000006.12 6 158144797 - SERAC1 NNNGCACCATATCATCACCA NGG 2 0.5402 Tier II
6 NC_000002.12 2 47572147 - MSH2 NNNCCATCATATCATCACCA NGG 2 0.416 Tier II
7 NC_000012.12 12 51512143 - SLC4A8 NNNGCATCATATCATCACTA NGG 2 0.3626 Tier II
8 NC_000016.10 16 71532445 - CHST4 NNNGGATCATCTCATCATCA NGG 2 0.2571 Tier II
9 NC_000016.10 16 47435096 - ITFG1 NNNGGATCATCTCATCACCT NGG 2 0.24 Tier II
10 NC_000005.10 5 10303649 + CMBL NNNGGATCATCTCTTCACCA NGG 2 0.2133 Tier II
11 NC_000003.12 3 167511613 + WDR49 NNNGGATCAGATCACCACCA NGG 2 0.1364 Tier II
12 NC_000003.12 3 39071459 + WDR48 NNNGGATCATATCATCTCCA NGG 1 0.1333 Tier II
13 NC_000023.11 X 70774439 - TEX11 NNNAGATCATATCATCAGCA NGG 2 0.12 Tier II
14 NC_000002.12 2 72399522 - EXOC6B NNNGAATCATATCATCAGCA NGG 2 0.1156 Tier II
15 NC_000014.9 14 61214372 + PRKCH NNNGGATCATATGATCATCA NGG 2 0.0877 Tier II
16 NC_000014.9 14 61209611 - PRKCH NNNGGATCATATGATCACTA NGG 2 0.0629 Tier II
17 NC_000011.10 11 85517504 - DLG2 NNNTGATCATATCATCACCA NGA 2 0.0253 Tier II
18 NC_000003.12 3 175347607 - NAALADL2 NNNGGATCATATCATCATCA NTG 2 0.025 Tier II
19 NC_000009.12 9 125151019 + PRPS1P2 NNNAGATCATATTATCACCA NGG 2 0.63 Tier III
20 NC_000006.12 6 113197446 + LOC105377953 NNNGGATCAGATCATCACAA NGG 2 0.2143 Tier III
21 NC_000016.10 16 72605502 - LINC01572 NNNGGATCATATCATCTCCA NGG 1 0.1333 Tier III
22 NC_000020.11 20 17187393 - LOC105372544 NNNGGATCATTTAATCACCA NGG 2 0.1183 Tier III
23 NC_000001.11 1 101156203 - LOC101928334 NNNGGGTCATATGATCACCA NGG 2 0.0974 Tier III
24 NC_000004.12 4 117337165 + LINC02262 NNNGGATCATCTCATCACCC NGG 2 0.0909 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000078.6 12 57238802 + Prps1l3 NNNGGATCATATTATCACCA NGG 1 0.7 Tier I
2 NC_000086.7 X 140467742 + Prps1 NNNAGATCATATTATCACCA NGG 2 0.63 Tier I
3 NC_000077.6 11 77467820 + Coro6 NNNGGGTCATATCTTCACCA NGG 2 0.381 Tier I
4 NC_000082.6 16 6695593 - Rbfox1 NNNGAAACATATCATCACCA NGG 2 0.7583 Tier II
5 NC_000078.6 12 57238802 + Mipol1 NNNGGATCATATTATCACCA NGG 1 0.7 Tier II
6 NC_000073.6 7 16870387 - Prkd2 NNNGGATTATATTATCACCA NGG 2 0.6125 Tier II
7 NC_000074.6 8 73183844 + Large1 NNNGAATGATATCATCACCA NGG 2 0.5571 Tier II
8 NC_000077.6 11 105749878 - Tanc2 NNNGGATTATCTCATCACCA NGG 2 0.35 Tier II
9 NC_000084.6 18 67422630 + Afg3l2 NNNGCATCATCTCATCACCA NGG 2 0.3143 Tier II
10 NC_000086.7 X 51201855 + Mbnl3 NNNGTATCATATCATAACCA NGG 2 0.3 Tier II
11 NC_000085.6 19 4561112 - Pcx NNNGTATAATATCATCACCA NGG 2 0.195 Tier II
12 NC_000071.6 5 30127307 + Hadha NNNGGATCATTTCATCGCCA NGG 2 0.0543 Tier II
13 NC_000073.6 7 65997332 + Pcsk6 NNNGGATCATATCATCTGCA NGG 2 0.0178 Tier II
14 NC_000078.6 12 46088644 - Gm33811 NNNGCATCATATCATCATCA NGG 2 0.5051 Tier III
15 NC_000068.7 2 180294339 + Gm29943 NNNGGATCCTAACATCACCA NGG 2 0.4571 Tier III
16 NC_000081.6 15 74346578 + Gm19859 NNNGGATCATATCATCACAT NGG 2 0.2571 Tier III
Download CSV

Other clones with same target sequence:

(none)