Construct: sgRNA BRDN0001146981
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGAGTGCTTTCAAGCGACCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ALPK2 (115701)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77524
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000018.10 |
18 |
58537183 |
- |
ALPK2 |
NNNGTGCTTTCAAGCGACCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
160887420 |
- |
ATP10B |
NNNGTGTTTTCAAGCAACCA |
NGG |
2 |
0.8125 |
Tier II |
| 3 |
NC_000023.11 |
X |
57347769 |
+ |
FAAH2 |
NNNGTGCTTTCAGGAGACCA |
NGG |
2 |
0.1449 |
Tier II |
| 4 |
NC_000016.10 |
16 |
49512434 |
- |
ZNF423 |
NNNTTGCTTTCAAGAGACCA |
NGG |
2 |
0.0808 |
Tier II |
| 5 |
NC_000006.12 |
6 |
72689224 |
+ |
KCNQ5 |
NNNGTGCTTTCAAGCGTCAA |
NGG |
2 |
0.0571 |
Tier II |
| 6 |
NC_000004.12 |
4 |
13431702 |
- |
RAB28 |
NNNGTGCTTTCAGGGGACCA |
NGG |
2 |
0.0326 |
Tier II |
| 7 |
NC_000008.11 |
8 |
144848573 |
+ |
COMMD5 |
NNNCTGCTTTCAAGCCACCA |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000021.9 |
21 |
28193616 |
- |
LINC01695 |
NNNGTGCTTTCAGGGGACCA |
NGG |
2 |
0.0326 |
Tier III |
| 9 |
NC_000017.11 |
17 |
28220579 |
+ |
LOC105371710 |
NNNCTGCTTTCAAGCTACCA |
NGG |
2 |
0.0 |
Tier III |
| 10 |
NC_000014.9 |
14 |
34084181 |
+ |
LOC102724945 |
NNNGTGCTTTGAAGCCACCA |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
25987471 |
- |
P3h2 |
NNNGTGCTGTCAAGCGATCA |
NGG |
2 |
0.398 |
Tier II |
| 2 |
NC_000083.6 |
17 |
30531120 |
+ |
Btbd9 |
NNNGTACTTTCAAGAGACCA |
NGG |
2 |
0.2222 |
Tier II |
| 3 |
NC_000070.6 |
4 |
14714160 |
- |
Lrrc69 |
NNNGAGCTTTCAAGGGACCA |
NGG |
2 |
0.025 |
Tier II |
| 4 |
NC_000073.6 |
7 |
41094614 |
+ |
Gm9239 |
NNNGAGCTTTCAAGGGACCA |
NGG |
2 |
0.025 |
Tier II |
| 5 |
NC_000082.6 |
16 |
36068022 |
+ |
Fam162a |
NNNGTGCTTTCAAGTGACCA |
NAG |
2 |
0.0173 |
Tier II |
| 6 |
NC_000071.6 |
5 |
112991716 |
- |
Grk3 |
NNNGTGCTTTCTAGCCACCA |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000077.6 |
11 |
77246481 |
+ |
Ssh2 |
NNNGTGGTTTCAAGCCACCA |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000068.7 |
2 |
140365929 |
+ |
Sel1l2 |
NNNGTGGTTTCAAGCTACCA |
NGG |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
