Construct: sgRNA BRDN0001146985
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAGAGGCTGTCAAGTCGTCT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- HSPB8 (26353)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75641
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
119179438 |
- |
HSPB8 |
NNNAGGCTGTCAAGTCGTCT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
112815581 |
+ |
APC |
NNNAGGCTGACAAGTCATCT |
NGG |
2 |
0.8 |
Tier I |
| 3 |
NC_000012.12 |
12 |
91057867 |
+ |
KERA |
NNNAAGCTGTCAAGTCGTCT |
NTG |
2 |
0.0338 |
Tier II |
| 4 |
NC_000010.11 |
10 |
43318981 |
+ |
LOC105378271 |
NNNAGGCTGTCAGGTCATCT |
NGG |
2 |
0.6087 |
Tier III |
| 5 |
NC_000005.10 |
5 |
68818350 |
- |
LOC105379013 |
NNNAGTCTGCCAAGTCGTCT |
NGG |
2 |
0.3556 |
Tier III |
| 6 |
NC_000002.12 |
2 |
51519257 |
- |
NRXN1-DT |
NNNAGGCTGGCAAGTCTTCT |
NGG |
2 |
0.125 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
33411612 |
+ |
Zbtb34 |
NNNAGGCTGTGAAGCCGTCT |
NGG |
2 |
0.0682 |
Tier I |
| 2 |
NC_000071.6 |
5 |
116422355 |
+ |
Hspb8 |
NNNGGGCTGTCAAGTCGTCT |
NGA |
2 |
0.0434 |
Tier I |
| 3 |
NC_000077.6 |
11 |
89717991 |
- |
Ankfn1 |
NNNAGTTTGTCAAGTCGTCT |
NGG |
2 |
0.5417 |
Tier II |
| 4 |
NC_000071.6 |
5 |
48129259 |
+ |
Slit2 |
NNNAGGCTGTCAGGTCCTCT |
NGG |
2 |
0.1535 |
Tier II |
| 5 |
NC_000075.6 |
9 |
53606769 |
+ |
Acat1 |
NNNGGGCTGTCAAGTCCTCT |
NGG |
2 |
0.1471 |
Tier II |
| 6 |
NC_000076.6 |
10 |
127035682 |
- |
Eef1akmt3 |
NNNAGGGTGTCATGTCGTCT |
NGG |
2 |
0.1412 |
Tier II |
| 7 |
NC_000072.6 |
6 |
55273962 |
- |
Mindy4 |
NNNAGGCTGTCAAGAGGTCT |
NGG |
2 |
0.0891 |
Tier II |
| 8 |
NC_000077.6 |
11 |
35876246 |
- |
Wwc1 |
NNNAGGCTGTCAAGTGGGCT |
NGG |
2 |
0.0513 |
Tier II |
| 9 |
NC_000067.6 |
1 |
134338532 |
+ |
Ppfia4 |
NNNAGGCTGTCAAGTCTTCG |
NGG |
2 |
0.0441 |
Tier II |
| 10 |
NC_000070.6 |
4 |
131700269 |
- |
Gm42321 |
NNNAGGCTGTCAAGTCTTCT |
NGG |
1 |
0.25 |
Tier III |
| 11 |
NC_000072.6 |
6 |
70581046 |
- |
Igk |
NNNTGGCTGTCTAGTCGTCT |
NGG |
2 |
0.2121 |
Tier III |
Other clones with same target sequence:
(none)
