Construct: sgRNA BRDN0001146991
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCAGGCGGATGTTGTCATCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EEF2K (29904)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77855
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000016.10 |
16 |
22256873 |
- |
EEF2K |
NNNGGCGGATGTTGTCATCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
61889893 |
+ |
INTS2 |
NNNGGCGGATGTTGTCACCC |
NGG |
2 |
0.1837 |
Tier I |
| 3 |
NC_000001.11 |
1 |
15943153 |
+ |
ZBTB17 |
NNNGGCGGATGTTGTCGTGG |
NGG |
2 |
0.0221 |
Tier I |
| 4 |
NC_000008.11 |
8 |
143065115 |
+ |
LY6S |
NNNGATGGATGTTGTCATCG |
NGG |
2 |
0.8048 |
Tier II |
| 5 |
NC_000005.10 |
5 |
160269659 |
+ |
CCNJL |
NNNGGCTGATGTTTTCATCG |
NGG |
2 |
0.1524 |
Tier II |
| 6 |
NC_000011.10 |
11 |
884370 |
+ |
CHID1 |
NNNGGGGGATGTGGTCATCG |
NGG |
2 |
0.1304 |
Tier II |
| 7 |
NC_000002.12 |
2 |
159893703 |
- |
LY75 |
NNNGGCGGATGGTGTCATGG |
NGG |
2 |
0.0625 |
Tier II |
| 8 |
NC_000002.12 |
2 |
159893703 |
- |
LY75-CD302 |
NNNGGCGGATGGTGTCATGG |
NGG |
2 |
0.0625 |
Tier II |
| 9 |
NC_000005.10 |
5 |
160269659 |
+ |
LOC105377691 |
NNNGGCTGATGTTTTCATCG |
NGG |
2 |
0.1524 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
23977570 |
- |
Bcl6 |
NNNGACGGACGTTGTCATCG |
NGG |
2 |
0.4622 |
Tier I |
| 2 |
NC_000073.6 |
7 |
120884783 |
- |
Eef2k |
NNNGTCGGATGTTGTCATCA |
NGG |
2 |
0.2813 |
Tier I |
| 3 |
NC_000081.6 |
15 |
85978877 |
+ |
Celsr1 |
NNNGGCAGATGTTGTCATCG |
NAG |
2 |
0.2593 |
Tier I |
| 4 |
NC_000067.6 |
1 |
89488760 |
+ |
Agap1 |
NNNAGAGGATGTTGTCATCG |
NGG |
2 |
0.8357 |
Tier II |
| 5 |
NC_000078.6 |
12 |
100051692 |
- |
Kcnk13 |
NNNGGCAGATGTTGTCATTG |
NGG |
2 |
0.4615 |
Tier II |
| 6 |
NC_000073.6 |
7 |
140102425 |
+ |
Fuom |
NNNGGCGGGTGTTGTCATCC |
NGG |
2 |
0.2857 |
Tier II |
| 7 |
NC_000068.7 |
2 |
168266934 |
- |
Kcng1 |
NNNGGCGGATATGGTCATCG |
NGG |
2 |
0.2609 |
Tier II |
| 8 |
NC_000078.6 |
12 |
100051692 |
- |
Gm51957 |
NNNGGCAGATGTTGTCATTG |
NGG |
2 |
0.4615 |
Tier III |
Other clones with same target sequence:
(none)
