Construct: sgRNA BRDN0001146992
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCGAGTAGGTGACGTCCGAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EPHA10 (284656)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76096
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
37753127 |
+ |
EPHA10 |
NNNAGTAGGTGACGTCCGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
120794216 |
+ |
GLI2 |
NNNAGGAGGTGACGTCTGAG |
NGG |
2 |
0.2667 |
Tier II |
| 3 |
NC_000022.11 |
22 |
33223675 |
- |
LARGE1 |
NNNAGTAGGTGACCTCTGAG |
NGG |
2 |
0.2 |
Tier II |
| 4 |
NC_000001.11 |
1 |
25299631 |
+ |
RHD |
NNNAGGAGGTGACGCCCGAG |
NGG |
2 |
0.1558 |
Tier II |
| 5 |
NC_000001.11 |
1 |
25299631 |
+ |
RSRP1 |
NNNAGGAGGTGACGCCCGAG |
NGG |
2 |
0.1558 |
Tier II |
| 6 |
NC_000001.11 |
1 |
25393758 |
- |
RHCE |
NNNAGGAGGTGACGCCCGAG |
NGG |
2 |
0.1558 |
Tier II |
| 7 |
NC_000007.14 |
7 |
34018240 |
+ |
BMPER |
NNNAGTAGGTGACTTCAGAG |
NGG |
2 |
0.1244 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000074.6 |
8 |
105889929 |
- |
Edc4 |
NNNCTTAGGTGACGTCCGAG |
NGG |
2 |
0.1059 |
Tier I |
| 2 |
NC_000070.6 |
4 |
124894804 |
- |
Epha10 |
NNNAGTAGGTGACGTCGGAG |
NGG |
1 |
0.0588 |
Tier I |
| 3 |
NC_000084.6 |
18 |
15716943 |
+ |
Chst9 |
NNNAGTAGGTGATGTCCAAG |
NGG |
2 |
0.4846 |
Tier II |
| 4 |
NC_000082.6 |
16 |
93972987 |
- |
Cldn14 |
NNNAGTTGGAGACGTCCGAG |
NGG |
2 |
0.375 |
Tier II |
| 5 |
NC_000078.6 |
12 |
119408156 |
- |
Macc1 |
NNNAGTTGGTGACTTCCGAG |
NGG |
2 |
0.1167 |
Tier II |
| 6 |
NC_000075.6 |
9 |
55511994 |
- |
Etfa |
NNNAGCAGGTGACGTCCGAG |
NCG |
2 |
0.0974 |
Tier II |
| 7 |
NC_000072.6 |
6 |
134387322 |
- |
4933406J09Rik |
NNNAGTAGGTGACATCCTAG |
NGG |
2 |
0.5 |
Tier III |
Other clones with same target sequence:
(none)
