Construct: sgRNA BRDN0001146999

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCTGAGAGGGTGTAGAACTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RBKS (64080)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77042

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 27832728 + RBKS NNNGAGAGGGTGTAGAACTG NGG 0 1.0 Tier I
2 NC_000011.10 11 65582203 + EHBP1L1 NNNGAGAGGGTGCAGAAGTG NGG 2 0.1053 Tier I
3 NC_000011.10 11 78174159 + KCTD21 NNNGAGAGGCTGTAGATCTG NGG 2 0.0533 Tier I
4 NC_000008.11 8 11439171 - FAM167A NNNGAAAGGTTGTAGAACTG NGG 2 0.8125 Tier II
5 NC_000023.11 X 84417680 + HDX NNNGAGAAGGTGCAGAACTG NGG 2 0.7895 Tier II
6 NC_000001.11 1 17250197 + PADI3 NNNGGGAGGGTGCAGAACTG NGG 2 0.5684 Tier II
7 NC_000008.11 8 65722284 - PDE7A NNNAAGAGCGTGTAGAACTG NGG 2 0.4846 Tier II
8 NC_000008.11 8 65722284 - MTFR1 NNNAAGAGCGTGTAGAACTG NGG 2 0.4846 Tier II
9 NC_000007.14 7 28600013 + CREB5 NNNGAGAGGGTGAAGAACTC NGG 2 0.2967 Tier II
10 NC_000007.14 7 28349731 + CREB5 NNNGAGAGGATGTACAACTG NGG 2 0.2545 Tier II
11 NC_000003.12 3 121988992 + ILDR1 NNNGTGAGGGTGAAGAACTG NGG 2 0.2517 Tier II
12 NC_000006.12 6 127518753 - MTCL3 NNNGAGTGGGTGTGGAACTG NGG 2 0.2042 Tier II
13 NC_000003.12 3 165024655 - SI NNNGAGAGGGTGGAGAATTG NGG 2 0.1677 Tier II
14 NC_000019.10 19 58573193 - MZF1 NNNGAGAGGGTGTAGAAACG NGG 2 0.1485 Tier II
15 NC_000004.12 4 47355848 + GABRB1 NNNGAGAGGCTGTAGAACAG NGG 2 0.1143 Tier II
16 NC_000022.11 22 38831744 - NPTXR NNNGAGCGGGTGGAGAACTG NGG 2 0.1141 Tier II
17 NC_000002.12 2 201300777 - FLACC1 NNNGAGAGGCTGTAGAACTG NAG 2 0.1037 Tier II
18 NC_000015.10 15 87971825 - NTRK3 NNNGAGAGGGTGTGGAGCTG NGG 2 0.0824 Tier II
19 NC_000007.14 7 18639274 + HDAC9 NNNGAGTGGGTGTAGAGCTG NGG 2 0.0772 Tier II
20 NC_000002.12 2 118983462 - MARCO NNNGAGAGGGTGTAAAACTG NGC 2 0.0209 Tier II
21 NC_000017.11 17 17715063 + RAI1 NNNGAGAGGGTGTAGAACTG NCC 2 0.0 Tier II
22 NC_000012.12 12 63320168 + LINC03056 NNNGAGAAGGTGTAGAAATG NGG 2 0.5385 Tier III
23 NC_000007.14 7 69291621 - LOC105375345 NNNGAGAGGGAGTAGAATTG NGG 2 0.4821 Tier III
24 NC_000022.11 22 24475028 - ADORA2A-AS1 NNNGAGAGAGTGTAGAACTC NGG 2 0.2755 Tier III
25 NC_000003.12 3 121988992 + LOC101927010 NNNGTGAGGGTGAAGAACTG NGG 2 0.2517 Tier III
26 NC_000006.12 6 119611804 + LOC105377975 NNNCAGAGGCTGTAGAACTG NGG 2 0.2118 Tier III
27 NC_000006.12 6 127518753 - SOGA3-KIAA0408 NNNGAGTGGGTGTGGAACTG NGG 2 0.2042 Tier III
28 NC_000019.10 19 58573193 - MZF1-AS1 NNNGAGAGGGTGTAGAAACG NGG 2 0.1485 Tier III
29 NC_000004.12 4 14751326 + LINC00504 NNNGAGAGTGTGTAGAACTG NAG 2 0.1383 Tier III
30 NC_000002.12 2 237053793 - LOC93463 NNNGAGAGGGTTGAGAACTG NGG 2 0.1003 Tier III
31 NC_000002.12 2 237053793 - LOC105373950 NNNGAGAGGGTTGAGAACTG NGG 2 0.1003 Tier III
32 NC_000002.12 2 237053793 - COPS8-DT NNNGAGAGGGTTGAGAACTG NGG 2 0.1003 Tier III
33 NC_000017.11 17 17715063 + LOC124903943 NNNGAGAGGGTGTAGAACTG NCC 2 0.0 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 31651851 + Rbks NNNGAGAGGGTATAGAACTG NGG 1 0.9333 Tier I
2 NC_000083.6 17 25845885 - Wdr90 NNNTAGAGGGTGTAGAACTG NGG 1 0.3636 Tier I
3 NC_000082.6 16 45825747 + Phldb2 NNNGAGAGGCTGCAGAACTG NGG 2 0.3158 Tier I
4 NC_000068.7 2 180177792 + Lama5 NNNGAGAGGGTGTAGGGCTG NGG 2 0.0339 Tier I
5 NC_000068.7 2 76935361 + Ttn NNNGAGTAGGTGTAGAACTG NGG 2 0.4375 Tier II
6 NC_000081.6 15 87568930 + Tafa5 NNNGGGAGGGTCTAGAACTG NGG 2 0.3812 Tier II
7 NC_000075.6 9 72706883 + Nedd4 NNNCAGAGAGTGTAGAACTG NGG 2 0.3403 Tier II
8 NC_000067.6 1 163988824 + BC055324 NNNGAGAGGGTGAAGAACTC NGG 2 0.2967 Tier II
9 NC_000081.6 15 96160294 + Gm38469 NNNGAGAAGGTGTACAACTG NGG 2 0.2727 Tier II
10 NC_000077.6 11 80077592 + Crlf3 NNNGAGAGGGTGTAGAACTG NAG 1 0.2593 Tier II
11 NC_000070.6 4 46680509 - Gabbr2 NNNGTGAGGGTGAAGAACTG NGG 2 0.2517 Tier II
12 NC_000077.6 11 35907543 - Wwc1 NNNGAGAGGGTGTGGAAATG NGG 2 0.2513 Tier II
13 NC_000068.7 2 168483652 + Nfatc2 NNNGTGAGTGTGTAGAACTG NGG 2 0.1939 Tier II
14 NC_000072.6 6 52177845 - Hoxa3 NNNGAGAGGCTGTGGAACTG NGG 2 0.1867 Tier II
15 NC_000085.6 19 5150445 - Pacs1 NNNGTGTGGGTGTAGAACTG NGG 2 0.1591 Tier II
16 NC_000068.7 2 121550136 - Frmd5 NNNGAGAGGGTGGAGAAATG NGG 2 0.1405 Tier II
17 NC_000072.6 6 90311738 + Chst13 NNNGAGAGGGTGAAGAGCTG NGG 2 0.1222 Tier II
18 NC_000070.6 4 43145313 - Unc13b NNNAAGAGGGTGTAGATCTG NGG 2 0.12 Tier II
19 NC_000075.6 9 107446439 - Cacna2d2 NNNGAGAGGCTGGAGAACTG NGG 2 0.1043 Tier II
20 NC_000085.6 19 55863463 - Tcf7l2 NNNGAGGGGGTGTAGAAGTG NGG 2 0.0941 Tier II
21 NC_000073.6 7 130695393 + Tacc2 NNNGAGAGGGTGAAGATCTG NGG 2 0.0923 Tier II
22 NC_000071.6 5 151212258 + Stard13 NNNGAGAGGGGGTAGGACTG NGG 2 0.0769 Tier II
23 NC_000075.6 9 109942131 + Map4 NNNGAGAGGGGGTAGATCTG NGG 2 0.0533 Tier II
24 NC_000082.6 16 12738452 - LOC108167340 NNNCAGAGGGTGTAGAACTG NGA 2 0.0368 Tier II
25 NC_000080.6 14 59678046 - Atp8a2 NNNGAGAGGGTGTAGAAGTG NCG 2 0.0143 Tier II
26 NC_000076.6 10 101762612 - Mgat4c NNNGAGAGGGAGTAGTACTG NGG 2 0.0 Tier II
27 NC_000071.6 5 31651851 + Gm38424 NNNGAGAGGGTATAGAACTG NGG 1 0.9333 Tier III
28 NC_000082.6 16 31528235 - Gm34032 NNNGAGATGGTGTTGAACTG NGG 2 0.3333 Tier III
29 NC_000072.6 6 52177845 - 5730596B20Rik NNNGAGAGGCTGTGGAACTG NGG 2 0.1867 Tier III
30 NC_000072.6 6 52177845 - LOC115490409 NNNGAGAGGCTGTGGAACTG NGG 2 0.1867 Tier III
31 NC_000073.6 7 101549145 - Gm19765 NNNGAGAGGATGTAGATCTG NGG 2 0.1244 Tier III
32 NC_000070.6 4 111704408 + Gm33192 NNNAAGAGGGTGTAGATCTG NGG 2 0.12 Tier III
Download CSV

Other clones with same target sequence:

(none)