Construct: sgRNA BRDN0001147001
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AATCAAGTTCGAGCACAACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP3K3 (4215)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76018
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
63646062 |
+ |
MAP3K3 |
NNNCAAGTTCGAGCACAACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
19545274 |
+ |
CILP2 |
NNNCAAGTACGAGTACAACG |
NGG |
2 |
0.681 |
Tier I |
| 3 |
NC_000018.10 |
18 |
34115131 |
+ |
NOL4 |
NNNCAAGTTCGAGCACAAAG |
NTG |
2 |
0.0167 |
Tier II |
| 4 |
NC_000011.10 |
11 |
28630910 |
+ |
LINC02758 |
NNNGAAGTTCGAGCACAACA |
NGG |
2 |
0.4688 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
32961825 |
+ |
Rpl12 |
NNNCAAGTTCGACCCCAACG |
NGG |
2 |
0.1148 |
Tier I |
| 2 |
NC_000067.6 |
1 |
159895448 |
+ |
Tnr |
NNNCAAATTCAAGCACAACG |
NGG |
2 |
1.0 |
Tier II |
| 3 |
NC_000072.6 |
6 |
140431767 |
- |
Plekha5 |
NNNCAAGTTAGAGCACCACG |
NGG |
2 |
0.1529 |
Tier II |
| 4 |
NC_000075.6 |
9 |
88392236 |
- |
Snx14 |
NNNCAAGTTCTAGAACAACG |
NGG |
2 |
0.1346 |
Tier II |
| 5 |
NC_000067.6 |
1 |
66849581 |
- |
Acadl |
NNNCAAGTTCGACCCCAACG |
NGG |
2 |
0.1148 |
Tier II |
| 6 |
NC_000079.6 |
13 |
10082233 |
- |
Chrm3 |
NNNCAAGTTCGAGCAGAAAG |
NGG |
2 |
0.0659 |
Tier II |
| 7 |
NC_000078.6 |
12 |
69830167 |
+ |
Map4k5 |
NNNCAAGTTCGAGGCCAACG |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000082.6 |
16 |
29628575 |
- |
Opa1 |
NNNCAAGTTCGAGGCCAACG |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000067.6 |
1 |
66849581 |
- |
Gm15793 |
NNNCAAGTTCGACCCCAACG |
NGG |
2 |
0.1148 |
Tier III |
| 10 |
NC_000069.6 |
3 |
130068503 |
+ |
Gm9396 |
NNNCAAGTTCGACCCCAACG |
NGG |
2 |
0.1148 |
Tier III |
| 11 |
NC_000076.6 |
10 |
29699269 |
- |
Gm10275 |
NNNCAAGTTCGACCCCAACG |
NGG |
2 |
0.1148 |
Tier III |
| 12 |
NC_000076.6 |
10 |
29699269 |
- |
Gm46219 |
NNNCAAGTTCGACCCCAACG |
NGG |
2 |
0.1148 |
Tier III |
| 13 |
NC_000077.6 |
11 |
83027976 |
- |
Gm11425 |
NNNCAAGTTCGACCCCAACG |
NGG |
2 |
0.1148 |
Tier III |
| 14 |
NC_000078.6 |
12 |
69830167 |
+ |
4930512B01Rik |
NNNCAAGTTCGAGGCCAACG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
