Construct: sgRNA BRDN0001147006

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TATCTTGAAAAAAGGTACAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PGM2L1 (283209)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77147

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000011.10 11 74370938 + PGM2L1 NNNCTTGAAAAAAGGTACAC NGG 0 1.0 Tier I
2 NC_000009.12 9 83794727 + GKAP1 NNNTTTAAAAAAAGGTACAC NGG 2 0.8 Tier II
3 NC_000006.12 6 111783131 - FYN NNNCTTGACAAAAGATACAC NGG 2 0.5378 Tier II
4 NC_000003.12 3 185676226 + IGF2BP2 NNNCTTAAAAAAAGGTACAA NGG 2 0.5 Tier II
5 NC_000004.12 4 70918139 - MOB1B NNNCTAGAAAAAAGGTAAAC NGG 2 0.4667 Tier II
6 NC_000007.14 7 147819278 + CNTNAP2 NNNCTAGAAAAAAGGTACAA NGG 2 0.4333 Tier II
7 NC_000003.12 3 61001939 + FHIT NNNCTGGAAAAAGGGTACAC NGG 2 0.3727 Tier II
8 NC_000005.10 5 112235933 + EPB41L4A NNNCTTCTAAAAAGGTACAC NGG 2 0.2946 Tier II
9 NC_000003.12 3 63607680 + SYNPR NNNCATGAAAAAAGGTAAAC NGG 2 0.2692 Tier II
10 NC_000011.10 11 13717958 - FAR1 NNNCTTGAAAACAGATACAC NGG 2 0.2477 Tier II
11 NC_000002.12 2 80181496 - CTNNA2 NNNCCTGAAAAAAGGTGCAC NGG 2 0.1765 Tier II
12 NC_000006.12 6 7174456 - RREB1 NNNCTTAAAAAAAGGTCCAC NGG 2 0.1765 Tier II
13 NC_000002.12 2 54518616 - SPTBN1 NNNCTTGAAAAAGTGTACAC NGG 2 0.1739 Tier II
14 NC_000002.12 2 207087915 - KLF7 NNNCTTGAAAATAGGTACAA NGG 2 0.1667 Tier II
15 NC_000011.10 11 99875734 - CNTN5 NNNCTTTAAAAAAGGTACAC NAG 2 0.1481 Tier II
16 NC_000015.10 15 36679595 - CDIN1 NNNCTTGAAAAAAGGTAAAC NAG 2 0.1396 Tier II
17 NC_000003.12 3 170427870 + CLDN11 NNNCTTGAAAAAAGGTTCAC NGG 1 0.1333 Tier II
18 NC_000001.11 1 243326544 + SDCCAG8 NNNCTTGAAAAATGGTACAT NGG 2 0.09 Tier II
19 NC_000023.11 X 67558193 + AR NNNCTTGAAAAAAGTTACAT NGG 2 0.0429 Tier II
20 NC_000002.12 2 69664265 - ANXA4 NNNCTTGAAAAAAGGTACAT NGA 2 0.0208 Tier II
21 NC_000002.12 2 12728991 - TRIB2 NNNCTTGAAAAAATGTACAC NGC 2 0.0059 Tier II
22 NC_000007.14 7 146983401 + CNTNAP2 NNNCTTGAAAAAAGGTACAC NAT 2 0.0 Tier II
23 NC_000003.12 3 177651870 - LINC00578 NNNCTTGGAAAAAGGAACAC NGG 2 0.6667 Tier III
24 NC_000013.11 13 89441582 + LOC107984621 NNNCTGGAAAAAGGGTACAC NGG 2 0.3727 Tier III
25 NC_000004.12 4 136841221 - LINC02511 NNNCTTCAAAAAAGGTACTC NGG 2 0.3702 Tier III
26 NC_000005.10 5 105292853 + LOC105379110 NNNCTTGAAAAAAGGCACAA NGG 2 0.3333 Tier III
27 NC_000005.10 5 112235933 + LOC101927023 NNNCTTCTAAAAAGGTACAC NGG 2 0.2946 Tier III
28 NC_000002.12 2 54518616 - C9orf85P1 NNNCTTGAAAAAGTGTACAC NGG 2 0.1739 Tier III
29 NC_000015.10 15 36679595 - LOC124903466 NNNCTTGAAAAAAGGTAAAC NAG 2 0.1396 Tier III
30 NC_000006.12 6 76848875 - LOC105377862 NNNCTTGAAAAAAGGTTCAA NGG 2 0.0667 Tier III
31 NC_000002.12 2 187009945 + CALCRL-AS1 NNNCTTGAAAAAAGGTGCAT NGG 2 0.0529 Tier III
32 NC_000020.11 20 55715831 - LOC107984001 NNNCTTGAAAACAGGTCCAC NGG 2 0.0464 Tier III
33 NC_000012.12 12 20203420 + LOC105369686 NNNCTTGAAAAAAGGTTCAC NGT 2 0.0022 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 100253282 - Pgm2l1 NNNCTTGAGAAAAGGTAAAC NGG 2 0.359 Tier I
2 NC_000086.7 X 136506962 + Kir3dl2 NNNCTTGAAAAAAAATACAC NGG 2 0.7059 Tier II
3 NC_000070.6 4 19021346 + Cnbd1 NNNCTTGAAAAAAGGCACAC NGG 1 0.6667 Tier II
4 NC_000069.6 3 64991004 + Kcnab1 NNNCTTGAGTAAAGGTACAC NGG 2 0.5882 Tier II
5 NC_000077.6 11 75300441 + Rpa1 NNNCTTTAATAAAGGTACAC NGG 2 0.5042 Tier II
6 NC_000068.7 2 26844476 + Abo NNNCTTGAGAAAAGGCACAC NGG 2 0.4444 Tier II
7 NC_000067.6 1 78414224 - Sgpp2 NNNCTTGTAAAAAGGTACAC NGG 1 0.4286 Tier II
8 NC_000069.6 3 145319660 - Col24a1 NNNCTTGAAAGAAGGTATAC NGG 2 0.4179 Tier II
9 NC_000067.6 1 162704330 - Prrc2c NNNCTGGAAAGAAGGTACAC NGG 2 0.3714 Tier II
10 NC_000081.6 15 100723975 + Galnt6 NNNCTTGAAGGAAGGTACAC NGG 2 0.3611 Tier II
11 NC_000082.6 16 59614431 + Arl6 NNNCTTGCAAAAAGGCACAC NGG 2 0.2857 Tier II
12 NC_000069.6 3 125436851 - Ndst4 NNNCTTGAAAAGTGGTACAC NGG 2 0.2167 Tier II
13 NC_000080.6 14 70407880 - Piwil2 NNNCTTGAAGAAAGGTACAT NGG 2 0.1667 Tier II
14 NC_000079.6 13 42833558 + Phactr1 NNNGTTGAAAAAAGGTACAT NGG 2 0.15 Tier II
15 NC_000079.6 13 48844742 - Phf2 NNNCTTGAAATAACGTACAC NGG 2 0.1319 Tier II
16 NC_000077.6 11 29202218 - Ppp4r3b NNNCTTGAAACAATGTACAC NGG 2 0.1067 Tier II
17 NC_000067.6 1 192717612 - Hhat NNNCTTGGAAAAAGGTAGAC NGG 2 0.0978 Tier II
18 NC_000077.6 11 52969610 - Fstl4 NNNCTTGAAAAAAGGTGCAT NGG 2 0.0529 Tier II
19 NC_000074.6 8 25958564 - Hgsnat NNNCTTGAAAACAGGTACAC NTG 2 0.0103 Tier II
20 NC_000075.6 9 100173251 + 4930540E01Rik NNNCTTGAAAAGAGGCACAC NGG 2 0.4815 Tier III
21 NC_000067.6 1 78414224 - Gm19072 NNNCTTGTAAAAAGGTACAC NGG 1 0.4286 Tier III
22 NC_000070.6 4 137246982 + Gm31820 NNNCTTGAAGAAAGGTACAA NGG 2 0.2778 Tier III
23 NC_000068.7 2 16983236 + 4930515L03Rik NNNCTGGAAAAAATGTACAC NGG 2 0.1524 Tier III
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Other clones with same target sequence:

(none)