Construct: sgRNA BRDN0001147008
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGGCTGGCTGACCTTAACGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EXOSC10 (5394)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77987
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
11087453 |
+ |
EXOSC10 |
NNNCTGGCTGACCTTAACGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
8376688 |
- |
PRAG1 |
NNNCTGGCTGACCTTAGTGA |
NGG |
2 |
0.1134 |
Tier I |
| 3 |
NC_000005.10 |
5 |
11193895 |
+ |
CTNND2 |
NNNTTGGCTGACCTTAAAGA |
NGG |
2 |
0.4308 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
148564739 |
- |
Exosc10 |
NNNAGGGCTGACCTTAACGA |
NGG |
2 |
0.5389 |
Tier I |
| 2 |
NC_000082.6 |
16 |
57430342 |
- |
Cmss1 |
NNNCTGGCTTACCTTAATGA |
NGG |
2 |
0.5223 |
Tier II |
| 3 |
NC_000082.6 |
16 |
57430342 |
- |
Filip1l |
NNNCTGGCTTACCTTAATGA |
NGG |
2 |
0.5223 |
Tier II |
| 4 |
NC_000079.6 |
13 |
113607085 |
- |
Snx18 |
NNNCTGGCTGTCCTAAACGA |
NGG |
2 |
0.1781 |
Tier II |
| 5 |
NC_000078.6 |
12 |
102473804 |
+ |
Golga5 |
NNNCTGGCTGACCTTACCGG |
NGG |
2 |
0.1349 |
Tier II |
| 6 |
NC_000083.6 |
17 |
71472861 |
+ |
Smchd1 |
NNNCTGGCTGCCCTCAACGA |
NGG |
2 |
0.1091 |
Tier II |
| 7 |
NC_000084.6 |
18 |
4557292 |
- |
Gm41658 |
NNNCTGGCTGACCTTAAAGA |
NGG |
1 |
0.5385 |
Tier III |
Other clones with same target sequence:
(none)
