Construct: sgRNA BRDN0001147010

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACAGTCAGGAATTAATTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP3K5 (4217)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76653

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000006.12 6 136669346 - MAP3K5 NNNGTCAGGAATTAATTATG NGG 0 1.0 Tier I
2 NC_000006.12 6 39387199 + KIF6 NNNGTAAGGAATTAATTATG NGG 1 0.9286 Tier II
3 NC_000014.9 14 22922576 + PRMT5 NNNGTCAGGAAATAATTATG NGG 1 0.8 Tier II
4 NC_000020.11 20 15245424 + MACROD2 NNNCTCAGGAACTAATTATG NGG 2 0.5015 Tier II
5 NC_000018.10 18 37493129 - CELF4 NNNGTCAGAAATTTATTATG NGG 2 0.3429 Tier II
6 NC_000006.12 6 17477691 - CAP2 NNNGTCAGGACATAATTATG NGG 2 0.32 Tier II
7 NC_000001.11 1 27291888 + WDTC1 NNNGTTAGGAATTAATTAAG NGG 2 0.2653 Tier II
8 NC_000006.12 6 163118064 + PACRG NNNGTCAGTAATTCATTATG NGG 2 0.1143 Tier II
9 NC_000007.14 7 36892671 - ELMO1 NNNGACAGGAATTCATTATG NGG 2 0.1071 Tier II
10 NC_000002.12 2 26255864 - HADHB NNNGACAGGAATTATTTATG NGG 2 0.1 Tier II
11 NC_000002.12 2 143583211 + ARHGAP15 NNNGTCAGGAATTATTTAAG NGG 2 0.0571 Tier II
12 NC_000014.9 14 67059924 + GPHN NNNGTCAGGAATTAACTATG NGA 2 0.0463 Tier II
13 NC_000023.11 X 18757929 + PPEF1 NNNGGCAGGAATTAATTATG NGA 2 0.0444 Tier II
14 NC_000005.10 5 65014769 + CWC27 NNNGTCAGGAATTTATTATG NTG 2 0.0208 Tier II
15 NC_000010.11 10 100007814 - DNMBP NNNGTCAGGAGTTAATTATG NGT 2 0.0105 Tier II
16 NC_000002.12 2 177797471 - PDE11A NNNGTCAGGCATTAATTATG NGT 2 0.0054 Tier II
17 NC_000006.12 6 39387199 + LOC107986594 NNNGTAAGGAATTAATTATG NGG 1 0.9286 Tier III
18 NC_000010.11 10 65695821 + LINC01515 NNNGTCAGGAACTAATTATA NGG 2 0.8882 Tier III
19 NC_000014.9 14 22922576 + PRMT5-AS1 NNNGTCAGGAAATAATTATG NGG 1 0.8 Tier III
20 NC_000021.9 21 22033389 - LINC01687 NNNGGCAGGAATTAATTATC NGG 2 0.2743 Tier III
21 NC_000002.12 2 58701115 + LINC01122 NNNGTCAGGAATTAATTCTG NGG 1 0.1905 Tier III
22 NC_000007.14 7 36892671 - LOC105375235 NNNGACAGGAATTCATTATG NGG 2 0.1071 Tier III
23 NC_000014.9 14 22037002 + TRA NNNGTCAGGGATTAATGATG NGG 2 0.0654 Tier III
24 NC_000001.11 1 158878817 - PYHIN5P NNNGTAAGGAATTAATTATG NTG 2 0.0362 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000076.6 10 20046422 + Map3k5 NNNGTCAGGAATTAATTATG NAG 1 0.2593 Tier I
2 NC_000068.7 2 137010706 - Slx4ip NNNGTCAAGAATTAATTATG NGG 1 1.0 Tier II
3 NC_000080.6 14 34412720 - Bmpr1a NNNGTCAGGAGTTAATTATA NGG 2 0.6094 Tier II
4 NC_000073.6 7 132562792 + Oat NNNGTTAGAAATTAATTATG NGG 2 0.5969 Tier II
5 NC_000086.7 X 160640199 - Ppef1 NNNATCAGAAATTAATTATG NGG 2 0.5786 Tier II
6 NC_000086.7 X 72450354 - Gabra3 NNNGTCAGGAAATAATCATG NGG 2 0.5647 Tier II
7 NC_000082.6 16 18271851 + Dgcr8 NNNGTCTGGAACTAATTATG NGG 2 0.4145 Tier II
8 NC_000067.6 1 180689155 - Lin9 NNNGTCAGGAAATAATTTTG NGG 2 0.4 Tier II
9 NC_000078.6 12 117703428 - Rapgef5 NNNGTCAGGAATTTATTATC NGG 2 0.2286 Tier II
10 NC_000079.6 13 19119496 + Amph NNNGTCAGGAATCAATTATG NAG 2 0.2047 Tier II
11 NC_000078.6 12 31962456 + Prkar2b NNNGTAAGGAATTAATTCTG NGG 2 0.1769 Tier II
12 NC_000069.6 3 152639803 - Ak5 NNNCTCAGGCATTAATTATG NGG 2 0.1765 Tier II
13 NC_000085.6 19 28434251 + Glis3 NNNGTCAGGAAGTAAGTATG NGG 2 0.1731 Tier II
14 NC_000068.7 2 170008308 - Tshz2 NNNGTCAGGAATAAATTAGG NGG 2 0.1731 Tier II
15 NC_000084.6 18 57928394 - Slc12a2 NNNGTCAGGAATTTATTAAG NGG 2 0.1524 Tier II
16 NC_000077.6 11 80454854 + Psmd11 NNNGTCTGGAATTAATTATG NAG 2 0.1134 Tier II
17 NC_000076.6 10 88362948 - Dram1 NNNGACAGGAATTCATTATG NGG 2 0.1071 Tier II
18 NC_000072.6 6 39716460 - Braf NNNGTCAGGAATTCATTGTG NGG 2 0.0857 Tier II
19 NC_000086.7 X 145121961 - Rtl4 NNNTTCAGGAATTCATTATG NGG 2 0.0779 Tier II
20 NC_000086.7 X 160676446 - Ppef1 NNNGGCAGGAATTAATTATG NGA 2 0.0444 Tier II
21 NC_000068.7 2 141111676 + Macrod2 NNNGTCAGGAATTAATTATG NTG 1 0.039 Tier II
22 NC_000084.6 18 43661412 + Jakmip2 NNNGTCAGGAATTAATAATG NGT 2 0.0086 Tier II
23 NC_000079.6 13 93306310 + Homer1 NNNCTCAGGAATTAATTATG NGT 2 0.0085 Tier II
24 NC_000075.6 9 61039470 - Gm33699 NNNGTCAGGAACTAATCATG NGG 2 0.6687 Tier III
25 NC_000067.6 1 107939243 - D830032E09Rik NNNGTCAGGAATTAATTATT NAG 2 0.1815 Tier III
26 NC_000077.6 11 83668267 - Gm51868 NNNGACAGGAATTAATTATG NAG 2 0.1296 Tier III
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Other clones with same target sequence:

(none)