Construct: sgRNA BRDN0001147011

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGTTCATTCACACAAAGCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAGI1 (9223)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
78070

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 65430823 - MAGI1 NNNTCATTCACACAAAGCTG NGG 0 1.0 Tier I
2 NC_000015.10 15 29623392 + ENTREP2 NNNTCATCCTCACAAAGCTG NGG 2 0.8824 Tier II
3 NC_000020.11 20 43603174 - IFT52 NNNTCATTTTCACAAAGCTG NGG 2 0.7721 Tier II
4 NC_000014.9 14 92659718 - RIN3 NNNTCATTCACACAAAGTTA NGG 2 0.6027 Tier II
5 NC_000005.10 5 159052834 - EBF1 NNNTCATTGACACAAAGCTA NGG 2 0.5804 Tier II
6 NC_000016.10 16 77851191 + VAT1L NNNTAATTCACACAAAACTG NGG 2 0.5333 Tier II
7 NC_000006.12 6 46551275 - CYP39A1 NNNTCATACACACAAAGTTG NGG 2 0.5143 Tier II
8 NC_000004.12 4 70820304 + GRSF1 NNNTCATTTACACTAAGCTG NGG 2 0.4667 Tier II
9 NC_000010.11 10 126088950 - ADAM12 NNNTCATTCACATTAAGCTG NGG 2 0.3733 Tier II
10 NC_000017.11 17 37458981 - TADA2A NNNTCAATCACTCAAAGCTG NGG 2 0.2917 Tier II
11 NC_000011.10 11 90172333 + NAALAD2 NNNTCATTCATACAAAGCTA NGG 2 0.2885 Tier II
12 NC_000003.12 3 120059467 + GSK3B NNNGCCTTCACACAAAGCTG NGG 2 0.2841 Tier II
13 NC_000001.11 1 169970018 + KIFAP3 NNNTACTTCACACAAAGCTG NGG 2 0.2597 Tier II
14 NC_000017.11 17 33624064 + ASIC2 NNNTCATCCACACCAAGCTG NGG 2 0.2143 Tier II
15 NC_000020.11 20 4173298 - SMOX NNNTCATTCACACGAAGCTC NGG 2 0.2 Tier II
16 NC_000008.11 8 100017961 + RGS22 NNNTCACTCACACACAGCTG NGG 2 0.1875 Tier II
17 NC_000007.14 7 95976133 - DYNC1I1 NNNTTATTCACACAAACCTG NGG 2 0.1497 Tier II
18 NC_000001.11 1 57346736 + DAB1 NNNTCATTCACTAAAAGCTG NGG 2 0.1282 Tier II
19 NC_000006.12 6 56525955 - DST NNNTCATTTACAGAAAGCTG NGG 2 0.1193 Tier II
20 NC_000006.12 6 44294408 - TCTE1 NNNTCATTCACACTAAGGTG NGG 2 0.0711 Tier II
21 NC_000002.12 2 66514566 - MEIS1 NNNACATTCACAGAAAGCTG NGG 2 0.0649 Tier II
22 NC_000002.12 2 211935010 + ERBB4 NNNTCATTCACACATAGCAG NGG 2 0.0571 Tier II
23 NC_000014.9 14 67738849 - ZFYVE26 NNNTCATTCACAGAAAGCAG NGG 2 0.039 Tier II
24 NC_000017.11 17 57868694 + CUEDC1 NNNTCATTCACACATGGCTG NGG 2 0.0385 Tier II
25 NC_000007.14 7 86794986 + GRM3 NNNTCATACACACAAAGCTG NTG 2 0.0312 Tier II
26 NC_000007.14 7 134276477 + LRGUK NNNTCATTCACAGAAAGGTG NGG 2 0.0182 Tier II
27 NC_000007.14 7 134826924 + CALD1 NNNTCATTCCCACAAAGCTG NTG 2 0.013 Tier II
28 NC_000007.14 7 111438596 + IMMP2L NNNTTATTCACACAAAGCTG NGT 2 0.0103 Tier II
29 NC_000002.12 2 188549535 + GULP1 NNNTCATTCACACATAGCTG NTG 2 0.0078 Tier II
30 NC_000006.12 6 145536639 + EPM2A NNNTCATTCACACAAACCTG NGC 2 0.0052 Tier II
31 NC_000013.11 13 38533218 + LINC00437 NNNTGATTAACACAAAGCTG NGG 2 0.5143 Tier III
32 NC_000004.12 4 23373548 + LOC105374523 NNNTCCTTCACACAAAGCTG NGG 1 0.4545 Tier III
33 NC_000004.12 4 23373548 + LOC105374524 NNNTCCTTCACACAAAGCTG NGG 1 0.4545 Tier III
34 NC_000004.12 4 3960609 + LOC105374358 NNNTCAGTCACACAAAGCTC NGG 2 0.2521 Tier III
35 NC_000003.12 3 157231601 - LOC101928236 NNNTCACTCATACAAAGCTG NGG 2 0.2115 Tier III
36 NC_000007.14 7 86794986 + GRM3-AS1 NNNTCATACACACAAAGCTG NTG 2 0.0312 Tier III
37 NC_000007.14 7 134826924 + LOC124901750 NNNTCATTCCCACAAAGCTG NTG 2 0.013 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000083.6 17 31459901 - Pde9a NNNTCATGCACACAGAGCTG NGG 2 0.4767 Tier I
2 NC_000072.6 6 93747316 - Magi1 NNNTCATTCACACGAAGCTA NGG 2 0.4375 Tier I
3 NC_000071.6 5 20998263 - Ptpn12 NNNTCATTCACACAGAGCTG NTG 2 0.0253 Tier I
4 NC_000068.7 2 91478294 - Lrp4 NNNTCATCCACACAGAGCTG NGG 2 0.65 Tier II
5 NC_000083.6 17 43597371 + Pla2g7 NNNTCTTTCTCACAAAGCTG NGG 2 0.6303 Tier II
6 NC_000082.6 16 6758481 - Rbfox1 NNNTCAGCCACACAAAGCTG NGG 2 0.5882 Tier II
7 NC_000073.6 7 132094970 - Cpxm2 NNNGCATTCTCACAAAGCTG NGG 2 0.5515 Tier II
8 NC_000071.6 5 130017076 + Asl NNNTCATTCACACTAAGCTG NGG 1 0.5333 Tier II
9 NC_000076.6 10 36633508 - Hs3st5 NNNTGATTCTCACAAAGCTG NGG 2 0.5294 Tier II
10 NC_000081.6 15 75155560 - Ly6g NNNTCAGTCTCACAAAGCTG NGG 2 0.519 Tier II
11 NC_000076.6 10 105424214 + Tmtc2 NNNTCATTCAGACAAAGCTG NGG 1 0.25 Tier II
12 NC_000072.6 6 32858000 - Chchd3 NNNTCATTTACACAAAGCAG NGG 2 0.25 Tier II
13 NC_000074.6 8 72751571 - Sin3b NNNTCATACATACAAAGCTG NGG 2 0.2462 Tier II
14 NC_000078.6 12 28499076 + Dcdc2c NNNTCCTTCACACAAAGATG NGG 2 0.2448 Tier II
15 NC_000085.6 19 57925933 - Atrnl1 NNNTCAATCACACAAACCTG NGG 2 0.2059 Tier II
16 NC_000080.6 14 115394885 + Gpc5 NNNTCATGCACACAAACCTG NGG 2 0.1725 Tier II
17 NC_000071.6 5 21506448 - Fbxl13 NNNTCATTCTCACAAGGCTG NGG 2 0.1697 Tier II
18 NC_000081.6 15 72677127 + Trappc9 NNNACATTCACTCAAAGCTG NGG 2 0.1587 Tier II
19 NC_000074.6 8 124413182 + Pgbd5 NNNTCATTCACACATAGATG NGG 2 0.1077 Tier II
20 NC_000085.6 19 30164140 + Gldc NNNTGATTCACAGAAAGCTG NGG 2 0.0818 Tier II
21 NC_000082.6 16 10649015 - Clec16a NNNACATTCACAGAAAGCTG NGG 2 0.0649 Tier II
22 NC_000070.6 4 4774866 + Bpnt2 NNNTCATTCATACATAGCTG NGG 2 0.0615 Tier II
23 NC_000068.7 2 102080325 - Ldlrad3 NNNTCATTCACACCCAGCTG NGG 2 0.0584 Tier II
24 NC_000082.6 16 34565212 + Kalrn NNNTCATTCACACAAAGGAG NGG 2 0.0381 Tier II
25 NC_000085.6 19 42778872 + Hps1 NNNTCATTCACACACAGGTG NGG 2 0.0364 Tier II
26 NC_000076.6 10 53944169 + Man1a NNNTCATACACACAAAGCTG NGT 2 0.0129 Tier II
27 NC_000075.6 9 90211071 - Tbc1d2b NNNTCACTCACACAAAGCTG NGT 2 0.0111 Tier II
28 NC_000076.6 10 121860459 + Srgap1 NNNTCATTCACACAAAGCTC NGT 2 0.0069 Tier II
29 NC_000074.6 8 10861224 - Gm32540 NNNTCAAACACACAAAGCTG NGG 2 0.7 Tier III
30 NC_000080.6 14 33916950 - Gm30031 NNNTCACTCACACAAAGCTA NGG 2 0.6445 Tier III
31 NC_000080.6 14 68727938 + Gm46476 NNNTTATCCACACAAAGCTG NGG 2 0.6364 Tier III
32 NC_000067.6 1 26721438 - Gm31136 NNNTCCTTCACACAAAGCTT NGG 2 0.3182 Tier III
33 NC_000086.7 X 81397314 - LOC115489112 NNNTCATTCACACTAAGATG NGG 2 0.2872 Tier III
34 NC_000085.6 19 57925933 - Gm52385 NNNTCAATCACACAAACCTG NGG 2 0.2059 Tier III
35 NC_000067.6 1 59403194 + Gm33724 NNNTAATTCACACAAAGCTG NAG 2 0.1481 Tier III
36 NC_000070.6 4 23487640 + Gm31085 NNNTCATTCACAAATAGCTG NGG 2 0.0769 Tier III
37 NC_000082.6 16 10649015 - Gm52264 NNNACATTCACAGAAAGCTG NGG 2 0.0649 Tier III
38 NC_000073.6 7 46979120 - Gm9999 NNNTCATTCAGACAAAGCTG NAG 2 0.0648 Tier III
39 NC_000076.6 10 109630017 + Gm40757 NNNTCATTCAGACAAAGCTG NAG 2 0.0648 Tier III
40 NC_000074.6 8 37013459 - G630064G18Rik NNNTAATTCACACAAAGCTG NTG 2 0.0223 Tier III
41 NC_000072.6 6 92982600 - 9530026P05Rik NNNTCATTCACACAAAGCGG NGC 2 0.0056 Tier III
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Other clones with same target sequence:

(none)