Construct: sgRNA BRDN0001147011
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGTTCATTCACACAAAGCTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAGI1 (9223)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 78070
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000083.6 |
17 |
31459901 |
- |
Pde9a |
NNNTCATGCACACAGAGCTG |
NGG |
2 |
0.4767 |
Tier I |
2 |
NC_000072.6 |
6 |
93747316 |
- |
Magi1 |
NNNTCATTCACACGAAGCTA |
NGG |
2 |
0.4375 |
Tier I |
3 |
NC_000071.6 |
5 |
20998263 |
- |
Ptpn12 |
NNNTCATTCACACAGAGCTG |
NTG |
2 |
0.0253 |
Tier I |
4 |
NC_000068.7 |
2 |
91478294 |
- |
Lrp4 |
NNNTCATCCACACAGAGCTG |
NGG |
2 |
0.65 |
Tier II |
5 |
NC_000083.6 |
17 |
43597371 |
+ |
Pla2g7 |
NNNTCTTTCTCACAAAGCTG |
NGG |
2 |
0.6303 |
Tier II |
6 |
NC_000082.6 |
16 |
6758481 |
- |
Rbfox1 |
NNNTCAGCCACACAAAGCTG |
NGG |
2 |
0.5882 |
Tier II |
7 |
NC_000073.6 |
7 |
132094970 |
- |
Cpxm2 |
NNNGCATTCTCACAAAGCTG |
NGG |
2 |
0.5515 |
Tier II |
8 |
NC_000071.6 |
5 |
130017076 |
+ |
Asl |
NNNTCATTCACACTAAGCTG |
NGG |
1 |
0.5333 |
Tier II |
9 |
NC_000076.6 |
10 |
36633508 |
- |
Hs3st5 |
NNNTGATTCTCACAAAGCTG |
NGG |
2 |
0.5294 |
Tier II |
10 |
NC_000081.6 |
15 |
75155560 |
- |
Ly6g |
NNNTCAGTCTCACAAAGCTG |
NGG |
2 |
0.519 |
Tier II |
11 |
NC_000076.6 |
10 |
105424214 |
+ |
Tmtc2 |
NNNTCATTCAGACAAAGCTG |
NGG |
1 |
0.25 |
Tier II |
12 |
NC_000072.6 |
6 |
32858000 |
- |
Chchd3 |
NNNTCATTTACACAAAGCAG |
NGG |
2 |
0.25 |
Tier II |
13 |
NC_000074.6 |
8 |
72751571 |
- |
Sin3b |
NNNTCATACATACAAAGCTG |
NGG |
2 |
0.2462 |
Tier II |
14 |
NC_000078.6 |
12 |
28499076 |
+ |
Dcdc2c |
NNNTCCTTCACACAAAGATG |
NGG |
2 |
0.2448 |
Tier II |
15 |
NC_000085.6 |
19 |
57925933 |
- |
Atrnl1 |
NNNTCAATCACACAAACCTG |
NGG |
2 |
0.2059 |
Tier II |
16 |
NC_000080.6 |
14 |
115394885 |
+ |
Gpc5 |
NNNTCATGCACACAAACCTG |
NGG |
2 |
0.1725 |
Tier II |
17 |
NC_000071.6 |
5 |
21506448 |
- |
Fbxl13 |
NNNTCATTCTCACAAGGCTG |
NGG |
2 |
0.1697 |
Tier II |
18 |
NC_000081.6 |
15 |
72677127 |
+ |
Trappc9 |
NNNACATTCACTCAAAGCTG |
NGG |
2 |
0.1587 |
Tier II |
19 |
NC_000074.6 |
8 |
124413182 |
+ |
Pgbd5 |
NNNTCATTCACACATAGATG |
NGG |
2 |
0.1077 |
Tier II |
20 |
NC_000085.6 |
19 |
30164140 |
+ |
Gldc |
NNNTGATTCACAGAAAGCTG |
NGG |
2 |
0.0818 |
Tier II |
21 |
NC_000082.6 |
16 |
10649015 |
- |
Clec16a |
NNNACATTCACAGAAAGCTG |
NGG |
2 |
0.0649 |
Tier II |
22 |
NC_000070.6 |
4 |
4774866 |
+ |
Bpnt2 |
NNNTCATTCATACATAGCTG |
NGG |
2 |
0.0615 |
Tier II |
23 |
NC_000068.7 |
2 |
102080325 |
- |
Ldlrad3 |
NNNTCATTCACACCCAGCTG |
NGG |
2 |
0.0584 |
Tier II |
24 |
NC_000082.6 |
16 |
34565212 |
+ |
Kalrn |
NNNTCATTCACACAAAGGAG |
NGG |
2 |
0.0381 |
Tier II |
25 |
NC_000085.6 |
19 |
42778872 |
+ |
Hps1 |
NNNTCATTCACACACAGGTG |
NGG |
2 |
0.0364 |
Tier II |
26 |
NC_000076.6 |
10 |
53944169 |
+ |
Man1a |
NNNTCATACACACAAAGCTG |
NGT |
2 |
0.0129 |
Tier II |
27 |
NC_000075.6 |
9 |
90211071 |
- |
Tbc1d2b |
NNNTCACTCACACAAAGCTG |
NGT |
2 |
0.0111 |
Tier II |
28 |
NC_000076.6 |
10 |
121860459 |
+ |
Srgap1 |
NNNTCATTCACACAAAGCTC |
NGT |
2 |
0.0069 |
Tier II |
29 |
NC_000074.6 |
8 |
10861224 |
- |
Gm32540 |
NNNTCAAACACACAAAGCTG |
NGG |
2 |
0.7 |
Tier III |
30 |
NC_000080.6 |
14 |
33916950 |
- |
Gm30031 |
NNNTCACTCACACAAAGCTA |
NGG |
2 |
0.6445 |
Tier III |
31 |
NC_000080.6 |
14 |
68727938 |
+ |
Gm46476 |
NNNTTATCCACACAAAGCTG |
NGG |
2 |
0.6364 |
Tier III |
32 |
NC_000067.6 |
1 |
26721438 |
- |
Gm31136 |
NNNTCCTTCACACAAAGCTT |
NGG |
2 |
0.3182 |
Tier III |
33 |
NC_000086.7 |
X |
81397314 |
- |
LOC115489112 |
NNNTCATTCACACTAAGATG |
NGG |
2 |
0.2872 |
Tier III |
34 |
NC_000085.6 |
19 |
57925933 |
- |
Gm52385 |
NNNTCAATCACACAAACCTG |
NGG |
2 |
0.2059 |
Tier III |
35 |
NC_000067.6 |
1 |
59403194 |
+ |
Gm33724 |
NNNTAATTCACACAAAGCTG |
NAG |
2 |
0.1481 |
Tier III |
36 |
NC_000070.6 |
4 |
23487640 |
+ |
Gm31085 |
NNNTCATTCACAAATAGCTG |
NGG |
2 |
0.0769 |
Tier III |
37 |
NC_000082.6 |
16 |
10649015 |
- |
Gm52264 |
NNNACATTCACAGAAAGCTG |
NGG |
2 |
0.0649 |
Tier III |
38 |
NC_000073.6 |
7 |
46979120 |
- |
Gm9999 |
NNNTCATTCAGACAAAGCTG |
NAG |
2 |
0.0648 |
Tier III |
39 |
NC_000076.6 |
10 |
109630017 |
+ |
Gm40757 |
NNNTCATTCAGACAAAGCTG |
NAG |
2 |
0.0648 |
Tier III |
40 |
NC_000074.6 |
8 |
37013459 |
- |
G630064G18Rik |
NNNTAATTCACACAAAGCTG |
NTG |
2 |
0.0223 |
Tier III |
41 |
NC_000072.6 |
6 |
92982600 |
- |
9530026P05Rik |
NNNTCATTCACACAAAGCGG |
NGC |
2 |
0.0056 |
Tier III |
Other clones with same target sequence:
(none)