Construct: sgRNA BRDN0001147012

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TTGTCCTTTGGAAACCCAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FYN (2534)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77242

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000006.12 6 111704040 - FYN NNNTCCTTTGGAAACCCAAG NGG 0 1.0 Tier I
2 NC_000022.11 22 39599258 - CACNA1I NNNTCCTATAGAAACCCAAG NGG 2 0.7467 Tier II
3 NC_000015.10 15 98746723 - IGF1R NNNTTCTTTTGAAACCCAAG NGG 2 0.517 Tier II
4 NC_000019.10 19 3507878 + FZR1 NNNTCCTTCGGAATCCCAAG NGG 2 0.4923 Tier II
5 NC_000017.11 17 76048673 - SRP68 NNNTCCTTTAGAAACCCTAG NGG 2 0.4667 Tier II
6 NC_000008.11 8 144069887 - EXOSC4 NNNTTCTTTGGAAACCCAAT NGG 2 0.4455 Tier II
7 NC_000011.10 11 45187737 + PRDM11 NNNACCTTTGGGAACCCAAG NGG 2 0.3439 Tier II
8 NC_000002.12 2 134173122 + MGAT5 NNNTCCTTTGGAAACTCAAA NGG 2 0.2885 Tier II
9 NC_000002.12 2 130189092 - MZT2B NNNTCCTTTGGAATCCCAAC NGG 2 0.2286 Tier II
10 NC_000002.12 2 131485599 + MZT2A NNNTCCTTTGGAATCCCAAC NGG 2 0.2286 Tier II
11 NC_000008.11 8 76850233 - ZFHX4 NNNACCTTTGGAAACCAAAG NGG 2 0.2222 Tier II
12 NC_000016.10 16 23665093 + DCTN5 NNNTCCTCTGGAAACCCCAG NGG 2 0.1905 Tier II
13 NC_000005.10 5 132716070 - KIF3A NNNTCCTTTGGAAACTCATG NGG 2 0.1657 Tier II
14 NC_000011.10 11 131513354 - NTM NNNTCCTATGGAAACCCCAG NGG 2 0.1524 Tier II
15 NC_000009.12 9 116279446 + PAPPA NNNTCCTTTGGAAACCCGGG NGG 2 0.15 Tier II
16 NC_000004.12 4 94772584 - BMPR1B NNNTCCTTTGGAAAATCAAG NGG 2 0.0684 Tier II
17 NC_000020.11 20 47246991 - ZMYND8 NNNCCCTTTGGAAACCCAAG NGA 2 0.0449 Tier II
18 NC_000016.10 16 10947126 + CLEC16A NNNTTCTTTGGAAACCCAAG NTG 2 0.0248 Tier II
19 NC_000001.11 1 180250515 + LHX4 NNNACCTTTGGAAAGCCAAG NGG 2 0.0238 Tier II
20 NC_000020.11 20 49858669 + SLC9A8 NNNTGCTTTGGAAACCCAAG NTG 2 0.0234 Tier II
21 NC_000005.10 5 122312554 - SNCAIP NNNTCCTTTGGAAACCGAGG NGG 2 0.0221 Tier II
22 NC_000001.11 1 208105334 - PLXNA2 NNNTCCTTTGGAAAGCCAGG NGG 2 0.0188 Tier II
23 NC_000007.14 7 96510140 - SEM1 NNNTCCTGTGGAAACCCAAG NGC 2 0.0163 Tier II
24 NC_000008.11 8 38285990 + NSD3 NNNTCCTTTCGAAACCCAAG NGT 2 0.0065 Tier II
25 NC_000008.11 8 125545100 - LINC02964 NNNTCCTCTAGAAACCCAAG NGG 2 0.9333 Tier III
26 NC_000002.12 2 217283886 + DIRC3-AS1 NNNTCATTTGGAATCCCAAG NGG 2 0.4952 Tier III
27 NC_000001.11 1 25045851 - LINC02793 NNNGGCTTTGGAAACCCAAG NGG 2 0.375 Tier III
28 NC_000009.12 9 752806 + LOC124902109 NNNTCCTCTGGAAACCCAGG NGG 2 0.375 Tier III
29 NC_000001.11 1 166778125 + FMO11P NNNCCCTTTGGAAAACCAAG NGG 2 0.1438 Tier III
30 NC_000016.10 16 58439999 + LINC02137 NNNTCCTTGGGAACCCCAAG NGG 2 0.1327 Tier III
31 NC_000004.12 4 66057315 + LOC105377261 NNNTCCTTTTGAAACCGAAG NGG 2 0.0478 Tier III
32 NC_000004.12 4 155080152 + LOC105377501 NNNTACTTTGGAAACCCAAG NGA 2 0.0397 Tier III
33 NC_000007.14 7 96510140 - LOC105375412 NNNTCCTGTGGAAACCCAAG NGC 2 0.0163 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000076.6 10 39525464 + Fyn NNNTCCTTTGGAAACCCAAG NGG 0 1.0 Tier I
2 NC_000072.6 6 129703331 + Klri1 NNNTCCTTTGGACACCGAAG NGG 2 0.0124 Tier I
3 NC_000080.6 14 55898482 - Khnyn NNNTCCTGTGGAAACCCAAA NGG 2 0.6875 Tier II
4 NC_000080.6 14 55898482 - Sdr39u1 NNNTCCTGTGGAAACCCAAA NGG 2 0.6875 Tier II
5 NC_000076.6 10 27030584 + Lama2 NNNTCCTTTGAAAACCCATG NGG 2 0.5385 Tier II
6 NC_000081.6 15 71715528 + Fam135b NNNCCCTTGGGAAACCCAAG NGG 2 0.4006 Tier II
7 NC_000083.6 17 24832948 + Meiob NNNACCTGTGGAAACCCAAG NGG 2 0.3492 Tier II
8 NC_000070.6 4 41689571 + Cntfr NNNACCCTTGGAAACCCAAG NGG 2 0.3274 Tier II
9 NC_000070.6 4 42179102 - Ccl21b NNNACCCTTGGAAACCCAAG NGG 2 0.3274 Tier II
10 NT_187054.1 4 24070 - LOC108168680 NNNACCCTTGGAAACCCAAG NGG 2 0.3274 Tier II
11 NC_000072.6 6 97147101 + Eogt NNNTCCCTTGTAAACCCAAG NGG 2 0.2644 Tier II
12 NC_000073.6 7 127209440 - Mylpf NNNTCTTTTGGAAACCCAAG NAG 2 0.2407 Tier II
13 NC_000079.6 13 38165618 + Dsp NNNTCCTTTGGAATCCCAGG NGG 2 0.2 Tier II
14 NC_000077.6 11 79675591 - Rab11fip4 NNNTCGTTTGGAAACCCAGG NGG 2 0.1875 Tier II
15 NC_000067.6 1 166382544 - Tada1 NNNTCCTTTGTAAACCTAAG NGG 2 0.1795 Tier II
16 NC_000080.6 14 70024533 + Pebp4 NNNTCCTGTGGAAACCCACG NGG 2 0.1517 Tier II
17 NC_000071.6 5 71776978 - Gabrb1 NNNTCCTTTGGAAAACAAAG NGG 2 0.1037 Tier II
18 NC_000082.6 16 40251574 - Lsamp NNNTCCTTTGGCAACTCAAG NGG 2 0.081 Tier II
19 NC_000084.6 18 58040552 + Fbn2 NNNTCCTTTGGAAACCCAAC NCG 2 0.0459 Tier II
20 NC_000074.6 8 45559122 + Sorbs2 NNNTCCTTTGAAAACCCAAG NTG 2 0.039 Tier II
21 NC_000073.6 7 12794524 - Zscan18 NNNTTCTTTGGAAACCGAAG NGG 2 0.0374 Tier II
22 NC_000079.6 13 97180298 - Hexb NNNACCTTTGGAAACCCAAG NGA 2 0.0331 Tier II
23 NC_000079.6 13 97180298 - Gfm2 NNNACCTTTGGAAACCCAAG NGA 2 0.0331 Tier II
24 NC_000071.6 5 114307957 + Myo1h NNNTCCTTAGGAAACCCAAG NGC 2 0.0206 Tier II
25 NC_000081.6 15 48220879 - Csmd3 NNNTCCTTTGGAAAGCCAGG NGG 2 0.0188 Tier II
26 NC_000072.6 6 130049787 + Klra4 NNNTCCTATGGAAACCCAAG NGT 2 0.0129 Tier II
27 NC_000072.6 6 138397515 - Lmo3 NNNCCCTTTGGAAACCCAAG NGT 2 0.0104 Tier II
28 NC_000074.6 8 21473397 + Defa-ps17 NNNTCCTTTGGAAACCCAGA NGG 2 0.3516 Tier III
29 NC_000070.6 4 42676261 - 4930578G10Rik NNNACCCTTGGAAACCCAAG NGG 2 0.3274 Tier III
30 NC_000080.6 14 70024533 + Gm33524 NNNTCCTGTGGAAACCCACG NGG 2 0.1517 Tier III
31 NC_000085.6 19 35837731 + A830019P07Rik NNNTCCTTAGGAAATCCAAG NGG 2 0.0619 Tier III
32 NC_000081.6 15 9429401 - Gm5219 NNNTCCTTTGGAAACCTAAG NCG 2 0.05 Tier III
33 NC_000073.6 7 12794524 - Gm4247 NNNTTCTTTGGAAACCGAAG NGG 2 0.0374 Tier III
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Other clones with same target sequence:

(none)