Construct: sgRNA BRDN0001147013
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CATGGTACGATGTGATACAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ROCK1 (6093)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77596
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000018.10 |
18 |
21042688 |
- |
ROCK1 |
NNNGGTACGATGTGATACAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
87562773 |
- |
RARS2 |
NNNGCTAAGATGTGATACAG |
NGG |
2 |
0.5107 |
Tier I |
| 3 |
NC_000005.10 |
5 |
112989160 |
+ |
DCP2 |
NNNGGTAGGATGTGATACAG |
NAG |
2 |
0.1667 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000084.6 |
18 |
10132251 |
- |
Rock1 |
NNNGGTACGATGTGACACAG |
NAG |
2 |
0.1728 |
Tier I |
| 2 |
NC_000073.6 |
7 |
134017133 |
+ |
Adam12 |
NNNGGTACCATGTGAAACAG |
NGG |
2 |
0.4895 |
Tier II |
| 3 |
NC_000071.6 |
5 |
120759714 |
- |
Oas3 |
NNNGGTACGGTGCGATACAG |
NGG |
2 |
0.4386 |
Tier II |
| 4 |
NC_000071.6 |
5 |
72630894 |
+ |
Cnga1 |
NNNGGTACCAAGTGATACAG |
NGG |
2 |
0.4038 |
Tier II |
| 5 |
NC_000079.6 |
13 |
95059673 |
- |
Pde8b |
NNNGGTACGATGTGTTACAG |
NGA |
2 |
0.0139 |
Tier II |
| 6 |
NC_000082.6 |
16 |
30795047 |
- |
Gm33502 |
NNNGGCAGGATGTGATACAG |
NGG |
2 |
0.5844 |
Tier III |
| 7 |
NC_000079.6 |
13 |
13275229 |
+ |
Gm5374 |
NNNGGTACGATGCGATACAG |
NAG |
2 |
0.2047 |
Tier III |
| 8 |
NC_000079.6 |
13 |
60489130 |
+ |
A530001N23Rik |
NNNTGTACCATGTGATACAG |
NGG |
2 |
0.1958 |
Tier III |
| 9 |
NC_000079.6 |
13 |
95059673 |
- |
Gm41025 |
NNNGGTACGATGTGTTACAG |
NGA |
2 |
0.0139 |
Tier III |
Other clones with same target sequence:
(none)
