Construct: sgRNA BRDN0001147014

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTTTCAAGAAGCCCCCATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDK17 (5128)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76787

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000012.12 12 96324024 - CDK17 NNNTCAAGAAGCCCCCATTG NGG 0 1.0 Tier I
2 NC_000001.11 1 167125923 - STYXL2 NNNTCAGGAAGCCCTCATTG NGG 2 0.0471 Tier I
3 NC_000002.12 2 178597667 - TTN NNNTCAAGAAGCCACCATTG NGA 2 0.0243 Tier I
4 NC_000005.10 5 52899899 - ITGA1 NNNTCAAGAAGCCTCAATTG NGG 2 0.7333 Tier II
5 NC_000009.12 9 84918341 - NTRK2 NNNCAAAGAAGCCCCCATTG NGG 2 0.3697 Tier II
6 NC_000006.12 6 25577873 - CARMIL1 NNNTCAATAAGTCCCCATTG NGG 2 0.3365 Tier II
7 NC_000011.10 11 118081001 - TMPRSS4 NNNTCAAGAAGCTCCCATTC NGG 2 0.3 Tier II
8 NC_000011.10 11 118081001 - SMIM35 NNNTCAAGAAGCTCCCATTC NGG 2 0.3 Tier II
9 NC_000002.12 2 25129796 - EFR3B NNNTAAAGAAGCCCCCACTG NGG 2 0.2449 Tier II
10 NC_000012.12 12 11838970 + ETV6 NNNTCGAGAAGCCCCCAGTG NGG 2 0.2381 Tier II
11 NC_000002.12 2 31202764 + CAPN14 NNNTCAAGAAGGACCCATTG NGG 2 0.1709 Tier II
12 NC_000019.10 19 21084487 - ZNF714 NNNTCAAGAAGCACCCATTC NGG 2 0.1648 Tier II
13 NC_000008.11 8 27512464 + EPHX2 NNNTCAAGGAGCCCACATTG NGG 2 0.1481 Tier II
14 NC_000004.12 4 76512494 - SHROOM3 NNNTGAAGAAGCCCACATTG NGG 2 0.1333 Tier II
15 NC_000004.12 4 113114166 - ANK2 NNNTCAAAAAGCCCTCATTG NGG 2 0.0667 Tier II
16 NC_000010.11 10 67035022 + CTNNA3 NNNTCAAGAAGCCCTTATTG NGG 2 0.0205 Tier II
17 NC_000010.11 10 67035022 + LRRTM3 NNNTCAAGAAGCCCTTATTG NGG 2 0.0205 Tier II
18 NC_000003.12 3 128745926 - RAB7A NNNTCAAGAAGCCCACATTG NTG 2 0.0087 Tier II
19 NC_000002.12 2 43691960 + PLEKHH2 NNNTCAAGAAGCCCTCATTG NGT 2 0.0011 Tier II
20 NC_000001.11 1 81680937 - ADGRL2 NNNTCAAGAAGCTGCCATTG NGG 2 0.0 Tier II
21 NC_000008.11 8 12390886 - FAM66A NNNTCATGAGGCCCCCATTG NGG 2 0.2431 Tier III
22 NC_000022.11 22 24423231 - SPECC1L-ADORA2A NNNTTAAGAAGCCCCCATTG NCG 2 0.0682 Tier III
23 NC_000002.12 2 178597667 - TTN-AS1 NNNTCAAGAAGCCACCATTG NGA 2 0.0243 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000076.6 10 93212000 + Cdk17 NNNTCAAGAAGCCCCCGTTG NGG 1 0.1765 Tier I
2 NC_000074.6 8 31096325 - Dusp26 NNNTCAAGAAGCCCCGGTTG NGG 2 0.0271 Tier I
3 NC_000078.6 12 75927396 - Syne2 NNNTCAAGAAGCCCCCTTTG NGT 2 0.0022 Tier I
4 NC_000086.7 X 99067352 - Stard8 NNNTCAAGAAGCTCCCATGG NGG 2 0.175 Tier II
5 NC_000067.6 1 191180134 + Atf3 NNNTTAAGAAGCCCCCATGG NGG 2 0.1591 Tier II
6 NC_000083.6 17 84219478 - Thada NNNACAAGAAGCCCCCTTTG NGG 2 0.0635 Tier II
7 NC_000068.7 2 14273720 + Mrc1 NNNACAAGAAGCCCTCATTG NGG 2 0.0317 Tier II
8 NC_000075.6 9 18533709 + Muc16 NNNTCAATAAGCCCCCATTG NTG 2 0.0244 Tier II
9 NC_000068.7 2 3220727 - Fam171a1 NNNTCAAGAAGCCCCCAGTG NTG 2 0.013 Tier II
10 NC_000073.6 7 118406669 + Syt17 NNNTCAAGAAGCCCCCAGTG NTG 2 0.013 Tier II
11 NC_000080.6 14 53875906 - Tcra NNNTCAAGAAGCCTCCTTTG NGG 2 0.0978 Tier III
12 NC_000082.6 16 29834262 + Gm32679 NNNTGAAGAAGCCCCCTTTG NGG 2 0.08 Tier III
13 NC_000084.6 18 62865396 + Gm41752 NNNTCAAGAAGCCAACATTG NGG 2 0.0778 Tier III
14 NC_000069.6 3 50014487 - Gm40042 NNNTCATGAAGCCCCCATTG NTG 2 0.017 Tier III
15 NC_000077.6 11 34032085 + 4930469K13Rik NNNCCAAGAAGCCCCCATTG NGC 2 0.0144 Tier III
16 NC_000067.6 1 76016608 + Gm32622 NNNTCAAGAAGCCCCCATTT NGT 2 0.0113 Tier III
17 NC_000067.6 1 76016608 + Gm29065 NNNTCAAGAAGCCCCCATTT NGT 2 0.0113 Tier III
Download CSV

Other clones with same target sequence:

(none)