Construct: sgRNA BRDN0001147015
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CGTGATGATCCCTACAGACC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- RIOK3 (8780)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76540
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000018.10 |
18 |
23464309 |
+ |
RIOK3 |
NNNGATGATCCCTACAGACC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
206043979 |
+ |
INO80D |
NNNGAAGATCCCTTCAGACC |
NGG |
2 |
0.4622 |
Tier II |
| 3 |
NC_000023.11 |
X |
70358141 |
- |
KIF4A |
NNNGAGGATCACTACAGACC |
NGG |
2 |
0.4286 |
Tier II |
| 4 |
NC_000003.12 |
3 |
141271146 |
- |
PXYLP1 |
NNNGATGATACCTACAGATC |
NGG |
2 |
0.4 |
Tier II |
| 5 |
NC_000002.12 |
2 |
55339928 |
- |
CCDC88A |
NNNGAGGATCCCTTCAGACC |
NGG |
2 |
0.3048 |
Tier II |
| 6 |
NC_000003.12 |
3 |
22091494 |
- |
ZNF385D |
NNNGAAGATCTCTACAGACC |
NGG |
2 |
0.2667 |
Tier II |
| 7 |
NC_000020.11 |
20 |
18615102 |
+ |
DTD1 |
NNNGCTGATCCCTTCAGACC |
NGG |
2 |
0.2667 |
Tier II |
| 8 |
NC_000001.11 |
1 |
243452519 |
- |
SDCCAG8 |
NNNGAGGATCCCTACAGGCC |
NGG |
2 |
0.2286 |
Tier II |
| 9 |
NC_000009.12 |
9 |
95829238 |
- |
ERCC6L2-AS1 |
NNNGACGATCACTACAGACC |
NGG |
2 |
0.6818 |
Tier III |
| 10 |
NC_000016.10 |
16 |
13796971 |
+ |
LOC124903646 |
NNNGATGAGCCATACAGACC |
NGG |
2 |
0.4422 |
Tier III |
| 11 |
NC_000002.12 |
2 |
55339928 |
- |
LOC124907768 |
NNNGAGGATCCCTTCAGACC |
NGG |
2 |
0.3048 |
Tier III |
| 12 |
NC_000005.10 |
5 |
37859734 |
- |
GDNF-AS1 |
NNNGATGATCCCTACAGAAA |
NGG |
2 |
0.2143 |
Tier III |
| 13 |
NC_000008.11 |
8 |
144711438 |
+ |
LOC100996662 |
NNNGATGATCCCTACACTCC |
NGG |
2 |
0.1176 |
Tier III |
| 14 |
NC_000004.12 |
4 |
10742676 |
- |
LINC02498 |
NNNGATGATCACTATAGACC |
NGG |
2 |
0.05 |
Tier III |
| 15 |
NC_000005.10 |
5 |
36325224 |
+ |
LOC124900962 |
NNNGGTGATCCCTACAGACC |
NTG |
2 |
0.0281 |
Tier III |
| 16 |
NC_000002.12 |
2 |
34143696 |
- |
LINC01317 |
NNNGATGATCCCTTGAGACC |
NGG |
2 |
0.0267 |
Tier III |
| 17 |
NC_000016.10 |
16 |
80227810 |
+ |
DYNLRB2-AS1 |
NNNGATGATCCCTTGAGACC |
NGG |
2 |
0.0267 |
Tier III |
| 18 |
NC_000013.11 |
13 |
32902678 |
- |
LINC00423 |
NNNGATGAGCCCTACAGACC |
NGC |
2 |
0.0138 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000084.6 |
18 |
12137031 |
+ |
Riok3 |
NNNGACGATCCCTACAGACC |
NGG |
1 |
0.9091 |
Tier I |
| 2 |
NC_000068.7 |
2 |
31651864 |
+ |
Prdm12 |
NNNGATGATCCCTCCAGACC |
NGG |
1 |
0.2143 |
Tier I |
| 3 |
NC_000067.6 |
1 |
174431121 |
+ |
Olfr414 |
NNNGAGGATCCCTACAGCCC |
NGG |
2 |
0.1088 |
Tier I |
| 4 |
NC_000067.6 |
1 |
166667253 |
- |
Fmo9 |
NNNGATGTTCCCTCCAGACC |
NGG |
2 |
0.0918 |
Tier I |
| 5 |
NC_000069.6 |
3 |
135667763 |
- |
Nfkb1 |
NNNGATGATCCCTACGGAAC |
NGG |
2 |
0.0824 |
Tier I |
| 6 |
NC_000081.6 |
15 |
10347763 |
+ |
Prlr |
NNNGATGCTCCCTACAGCCC |
NGG |
2 |
0.0816 |
Tier II |
| 7 |
NC_000075.6 |
9 |
97715494 |
+ |
Clstn2 |
NNNGATGATCCCTACACAGC |
NGG |
2 |
0.0294 |
Tier II |
| 8 |
NC_000083.6 |
17 |
8973687 |
+ |
Pde10a |
NNNGATGATCCCGACAGACC |
NGA |
2 |
0.0181 |
Tier II |
| 9 |
NC_000071.6 |
5 |
107088640 |
+ |
Gm42146 |
NNNGATAATCCCTTCAGACC |
NGG |
2 |
0.5333 |
Tier III |
| 10 |
NC_000073.6 |
7 |
12182439 |
- |
Gm19201 |
NNNGATGATCCCCAAAGACC |
NGG |
2 |
0.1754 |
Tier III |
Other clones with same target sequence:
(none)
