Construct: sgRNA BRDN0001147016

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AACCTCTGTAATGACGACGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ABL2 (27)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77177

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 179109075 - ABL2 NNNCTCTGTAATGACGACGG NGG 0 1.0 Tier I
2 NC_000007.14 7 40511105 - SUGCT NNNCTCTGTAATGACGATGA NGG 2 0.6027 Tier II
3 NC_000001.11 1 30709601 - LOC124903888 NNNCTCTGTAATTACGATGG NGG 2 0.1929 Tier III
4 NC_000008.11 8 117301461 + LOC105375716 NNNCTCTGTAATGATGACTG NGG 2 0.0444 Tier III
5 NC_000008.11 8 117301461 + LOC105375717 NNNCTCTGTAATGATGACTG NGG 2 0.0444 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000086.7 X 20925506 - Cfp NNNTTCTGTAATGACGAAGG NGG 2 0.4308 Tier II
2 NC_000071.6 5 38486060 - Slc2a9 NNNCTCTGTAATGGCGGCGG NGG 2 0.0824 Tier II
3 NC_000071.6 5 39233855 + Gm40293 NNNCTCTGTAATGAGGAAGG NGG 2 0.0269 Tier III
Download CSV

Other clones with same target sequence:

(none)