Construct: sgRNA BRDN0001147018
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCATCGCTTGCAAACAGAGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- RPS6KA6 (27330)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75535
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
84106418 |
+ |
RPS6KA6 |
NNNTCGCTTGCAAACAGAGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000023.11 |
X |
20172799 |
+ |
RPS6KA3 |
NNNTCTCTTGCAAACAGAGT |
NGG |
1 |
0.6667 |
Tier I |
| 3 |
NC_000006.12 |
6 |
166448777 |
+ |
RPS6KA2 |
NNNTCGCTTGCACACTGAGT |
NGG |
2 |
0.0 |
Tier I |
| 4 |
NC_000017.11 |
17 |
68929462 |
+ |
ABCA8 |
NNNTCACATGCAAACAGAGT |
NGG |
2 |
0.8 |
Tier II |
| 5 |
NC_000010.11 |
10 |
86706402 |
- |
LDB3 |
NNNTGCCTTGCAAACAGAGT |
NGG |
2 |
0.4091 |
Tier II |
| 6 |
NC_000017.11 |
17 |
78683516 |
- |
CYTH1 |
NNNTAGCTTGCAGACAGAGT |
NGG |
2 |
0.3727 |
Tier II |
| 7 |
NC_000011.10 |
11 |
85209261 |
- |
DLG2 |
NNNTAGCTTGGAAACAGAGT |
NGG |
2 |
0.1429 |
Tier II |
| 8 |
NC_000018.10 |
18 |
79455266 |
- |
NFATC1 |
NNNTCGCTGGCAAACGGAGT |
NGG |
2 |
0.119 |
Tier II |
| 9 |
NC_000023.11 |
X |
8038542 |
- |
LOC107985675 |
NNNTCCCTTGCAAACAGAGT |
NGC |
2 |
0.0152 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
111409386 |
+ |
Rps6ka6 |
NNNTCGCTTGCAAACAGAAT |
NAG |
2 |
0.1852 |
Tier I |
| 2 |
NC_000077.6 |
11 |
54317139 |
+ |
Acsl6 |
NNNTCGCTTGCTAACATAGT |
NGG |
2 |
0.0833 |
Tier II |
| 3 |
NC_000086.7 |
X |
59556977 |
- |
Fgf13 |
NNNTCTCTTGCAAACAGAGC |
NGG |
2 |
0.0606 |
Tier II |
| 4 |
NC_000081.6 |
15 |
4041085 |
- |
Oxct1 |
NNNTCGCTTACAAACAGAGT |
NTG |
2 |
0.0364 |
Tier II |
| 5 |
NC_000071.6 |
5 |
115204028 |
+ |
Gm35515 |
NNNTGGCTTGCAAACAAAGT |
NGG |
2 |
0.56 |
Tier III |
Other clones with same target sequence:
(none)
