Construct: sgRNA BRDN0001147023
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGGAGGAAACGCCCTCGAGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DGUOK (1716)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76602
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
73926979 |
- |
DGUOK |
NNNAGGAAACGCCCTCGAGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000018.10 |
18 |
53684587 |
- |
LOC124904304 |
NNNAGGAAAAGCCCTCAAGT |
NGG |
2 |
0.8089 |
Tier II |
| 3 |
NC_000015.10 |
15 |
71426128 |
- |
THSD4 |
NNNAGGAAACGCCCTACAGT |
NGG |
2 |
0.2353 |
Tier II |
| 4 |
NC_000002.12 |
2 |
201276717 |
+ |
CASP8 |
NNNAGGAAACGACCCCGAGT |
NGG |
2 |
0.1948 |
Tier II |
| 5 |
NC_000007.14 |
7 |
2982221 |
+ |
CARD11 |
NNNAGTAAACGCCCTGGAGT |
NGG |
2 |
0.1026 |
Tier II |
| 6 |
NC_000002.12 |
2 |
206202879 |
- |
CMKLR2 |
NNNAGGAAACGCGCTGGAGT |
NGG |
2 |
0.021 |
Tier II |
| 7 |
NC_000021.9 |
21 |
46668019 |
+ |
LOC124905058 |
NNNAGGACCCGCCCTCGAGT |
NGG |
2 |
0.2449 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
108846800 |
- |
Celsr3 |
NNNTGGAAACGCCCTCGAGT |
NCG |
2 |
0.0682 |
Tier I |
| 2 |
NC_000068.7 |
2 |
111217448 |
+ |
A26c3 |
NNNAGGAAATGCCCTAGAGT |
NGG |
2 |
0.9412 |
Tier II |
| 3 |
NC_000074.6 |
8 |
110593150 |
- |
Hydin |
NNNAGGACACGCCCTCAAGT |
NGG |
2 |
0.4 |
Tier II |
| 4 |
NC_000078.6 |
12 |
104438955 |
+ |
Gm40562 |
NNNAGGAAATGCCCTCGAGT |
NGG |
1 |
0.9412 |
Tier III |
Other clones with same target sequence:
(none)
